Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"N-Ethylmaleimide"”.
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N-ethylmaleimide sensitive factor (NSF) is a regulator of plasma membrane rupture and necrotic cell death
… membrane rupture andnecrosis. We targeted N-ethylmaleimide sensitive factor (NSF) due to its requirement in SNARE recycling, lack of gene homologs, and redox sensitivity. Deletion of Nsf protected against membrane rupture induced by various necrotic stimuli, yet did not influence apoptosis, …
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Alcohol alters the expression of Soluble N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptors (SNAREs) and spontaneous γ-Aminobutyric Acid (GABA) release...
… machinery proteins that form the soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) complex. The proteins in this complex reside on the vesicle membrane (synaptotagmin 1 and synaptobrevin/vesicle-associated membrane protein, which is also known as VAMP) and the …
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Unraveling the Functions of Synaptotagmin and Munc13 in Neurotransmitter Release
… machinery includes three SNAREs (soluble N-ethylmaleimide sensitive factor adaptor protein receptor) and Munc18-1 as core components. Munc13, synaptotagmin and complexin underlie the exquisitely tight regulation of synaptic exocytosis. The SNAREs form the SNARE complex that brings synaptic …
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An evolutionarily conserved regulatory mechanism for endosomal membrane trafficking
… facilitated by the formation of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes; a process that is regulated by Sec1p/Munc18 (SM) proteins. Membrane fusion has been conserved through evolution and hence a lot or our knowledge about the molecular mechanism …
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Studies on some enzymatic properties of mitochondrial propionyl carboxylase
… The carboxylase is inhibited by PCMB, N-ethylmaleimide, and iodoacetamide; and the inhibition by PCMB can be almost completely reversed by GSH. The K<sub>m</sub> values for acetyl-CoA, propionyl-CoA, butyryl-CoA, valeryh-CoA, propionyl pantetheine, ATP, and HCOj were determined. The …
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Investigation of early endosomal sorting and budding
… EEA1. Des Weiteren wird die Funktion des N-Ethylmaleimide-sensitiven Faktors (NSF), eines bekannten Fusions-Kofaktors, benötigt, während endosomale Fusion selbst nicht erforderlich ist. Daraus wird geschlussfolgert, dass Docking und Fusion, sowie Sortierung und Abschnürung von Vesikeln auf …
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Green chemistry : dense carbon dioxide and water as environmentally benign reaction media
… of 9-hydroxymethylanthracene with N-ethylmaleimide in scCO₂ was to demonstrate the ability of scCO₂ to dramatically accelerate the rate of this reaction when compared to conventional solvents. Using spectroscopy to track the disappearance of the 9-hydroxymethylanthracene peak, it was …
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The Development of a Bacterial Biosensor Designed to Detect Oxidative Chemicals in Water: Correlating Sensor Relevance to Mammalian Brain Cells and Assessing Bacterial Cell Immobilization Strategies
… to a model oxidative electrophilic chemical, N-ethylmaleimide, correspond well to each other. We also showed that both responses are accompanied by the depletion of intracellular glutathione, which precedes the GGKE response in P. aeruginosa as well as mitochondrial damage in rat brain cells. …
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Active Center Structure and Sequence Studies on Bacterial Luciferase Utilizing the Essential Cysteine, Protease-Labile Region, and Delta Fragment
… modified at this cysteine with ('3)H-N-ethylmaleimide, N-terminal sequence analysis of the resulting fragments, and specific cleavage of (alpha) at the essential thiol caused by modification with 2-nitro-5-thiocyanatobenzoic acid, are consistent with a model which places this residue on …
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Protein-Protein Interactions That Regulate Neurotransmitter Release From Retinal Ribbon Synapses
… do not contain the t-SNARE (target-soluble N-ethylmaleimide-sensitive factor attachment protein receptor) syntaxin 1A that is found in conventional synapses of the nervous system. In contrast, ribbon synapses of the mammalian retina contain the related isoform, syntaxin 3B. Given that SNARE …
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Studies of the coenzyme binding site and essential sulfhydryl group of years 6-phosphogluconate dehydrogenase
… the enzyme was protected from N-ethylmaleimide inactivation and this protection was enhanced by the addition of NADPH or AADP.
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Conformational changes in the (Ca²⁺, Mg²⁺)-ATPase of sarcoplasmic reticulum during energy transduction
… (DTNB) and stopped flow analysis or 1-¹⁴C-N-ethylmaleimide (NEM). Kinetic reactivity with DTNB revealed a total of 20 thiol groups/1.5 x 10⁵ g of SR protein (rontaining 1 mole of ATPase protein) in the presence of sodium dodecyl sulphate, which constitute four kinetic classes. In native …
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Effects of Cysteine Modification on Microtubule-Motor Protein Function and Tubulin Assembly
… probing functionally important amino acids. N-ethylmaleimide (NEM) reacts readily with exposed sulfhydryl groups, and has previously been shown to inhibit the activity of MT-motor proteins and tubulin assembly. This project seeks to investigate the mechanisms by which NEM affects motor function …
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Single-molecule fluorescence resonance energy transfer study of SNARE-mediated membrane fusion
… some form of fusion. Proteins called soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) play a central role in fusion processes which is also regulated by many accessory proteins, such as synaptotagmin, complexin and Munc18. By a new lipid mixing method at the …
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Genomic Analysis of Human and Mouse Guanine-7-Methyltransferase with Active Site Characterization
… Enzyme activity was completely eliminated when N-ethylmaleimide (NEM) was added to the assay mixture. When the product of the reaction, S-adenosyl-L-homocysteine (SAH), was added at a concentration of 40uM the mouse enzyme retained 60% activity while enzyme isolated from Human Osteosarcoma (HOS) …
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Molecular Sensing with Protein and Solid-State Nanopores
… packaging domain of genomic RNA. Additionally, N-ethylmaleimide, which is known to inhibit the binding of NCp7 to a high-affinity SL3 RNA aptamer, will be used to demonstrate that the inhibition of the binding can be monitored in real time.</p> <p>Following these applications of the solid-state …
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Cryo-electron Microscopy of Reconstituted Membrane Fusion Systems
Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins are responsible for driving the fusion of biological membranes and are present across many different cell trafficiking systems. Synaptic SNARE proteins allow the release of neurotransmitters across synaptic …
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The role of SNAREs in chlamydial infection: Syntaxin 4 and SNAP-23 disrupt lipid droplets homeostasis
… hospedeira como as proteínas SNARE (Soluble N-ethylmaleimide-sensitive Factor Attachment Protein Receptor). Aqui usámos um modelo de infeção in vitro com Chlamydia trachomatis LGV L2 para estudar a função das SNAREs na aquisição de nutrientes. Os nossos resultados mostram que a SNAP-23 …
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Regulation of SNARE-Mediated Synaptic Vesicle Release by Synaptotagmins and Complexins
… Ca2+ channels. The SNARE (Soluble N-ethylmaleimide-sensitive-factor Attachment Protein Receptors) complex is the core component of the fusion machinery in the presynaptic terminal, as it forms a physical bridge between the vesicular membrane and the presynaptic target membrane that …
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The Inactivation of Neurohypophyseal Hormones by the Toad Bladder
… fraction was decreased by treatment with N-ethylmaleimide, by heating to 50° C for 5 minutes, and by cold storage at -20° C. The decrease in activity due to cold storage, but not that due to either of the other two treatments, was reversed by the addition of 1 mM cysteine shortly before …
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