Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 11 of 11 for “"N gene"”.
-
Controlling Tobacco Mosaic Virus in Tobacco through Resistance
… tabacum L.) and causes losses worldwide. The N gene is the most effective means of controlling TMV; however, this gene is associated with reduced yield and quality in flue-cured tobacco. The mode of inheritance of TMV resistance was determined in two tobacco introductions (TI) from N. tabacum …
-
The Inheritance of Resistance to Tobacco Mosaic Virus in Tobacco Introductions
… local lesion:1 mosaic ratio, indicating that the gene governing resistance in these three TI lines was a single, dominant trait. The F2 progeny of TIs 203, 407, 438, 450, 692, 1203, and 1467 failed to segregate, only mosaic plants were observed. This would indicate that the gene(s) controlling …
-
Studies on Transformation of Tomato(Solanum lycopersicum L.) and Arabidopsis thaliana using Chimerical constructs of varying Tospoviral Origin
… through transformation with conserved viral genes. Given the severity of Tospovirus diseases worldwide (particularly in tomato), PDR has the potential to garner large yield returns where pathogen populations have overcome the established resistance. Tomato breeding lines FLA7804, FLA8044, and …
-
The Utilization of the Hmg2 Inducible Promoter to Genetically Engineer Parasite Resistance in Tobacco
… nematode and Egyptian broomrape by expressing genes encoding parasite specific toxins under the control of parasite-responsive promoters using tobacco (Nicotiana tabacum L. cv. Xanthi). For nematode resistance, an anti-feeding strategy was employed utilizing the tomato proteinase inhibitor I …
-
Impatiens Necrotic Spot Virus Resistance in Transgenic Impatiens walleriana and Lycopersicon esculentum
… frame of INSV nucleocapsid protein (N). The N gene is expected to confer INSV resistance by RNA interference or gene silencing. The presence of transgenes was confirmed by PCR. Transgenic Impatiens was selfed for two generations. Transgenic Micro-Tom was selfed for 4 generations. Spinach was …
-
An evaluation of reverse transcription loop-mediated isothermal amplification (RTLAMP) for diagnosis of rabies.
… designed based on the most conserved region of N gene sequences covering various rabies virus isolates representative sequences from around Africa. Amplification products of the RTLAMP assay was monitored by visual colour change of fluorescent dye and by agarose gel electrophoresis. To evaluate …
-
An evaluation of reverse transcription loop-mediated isothermal amplification (RTLAMP) for diagnosis of rabies.
… designed based on the most conserved region of N gene sequences covering various rabies virus isolates representative sequences from around Africa. Amplification products of the RTLAMP assay was monitored by visual colour change of fluorescent dye and by agarose gel electrophoresis. To evaluate …
-
Laukinių ir naminių gyvūnų pasiutligės epidemiologija, diagnostika ir imunoprofilaktika Lietuvoje /
… (ORV) period between 2007 and 2011, the philogenetic analysis of RV isolates of domestic and wild animals in the sphere of N gene by means of the reverse transcription-polymerase chain reaction (RT-PCR) was carried out for the first time. In 2006-2011, the assessment of the efficacy of ORV in …
-
The Effects of Temperature On The Durability Of Resistance Of Soybean To Soybean Mosaic Virus
… similar to those employed in the research with N gene-tobacco mosaic virus studies. The soybean cultivars used were Lee 69, York, Kwanggyo, Ogden and PI96983, carrying the Rsv, Rsv1-y, Rsv1-k, Rsv1-t, and Rsv1 allles of Rsv1, respectively, and were selected to provide a range of reactions to …
-
Developing SINrep5 as a Viral Vector Platform to Test SARS-CoV-2 Vaccine Candidates
… Envelope-E, and Membrane-M. The N gene was subcloned into a mammalian SINrep5 plasmid (pmSINrep5) to test the vector’s protein expression in vitro. Vector transfections were optimized for protein expression and Virus-Like Particles (VLP) production. Techniques to purify, …
-
Engineering programmable nucleases for in vivo and in vitro applications
… by allowing precise and easy manipulation of genetic material. These reprogrammable nucleases hold immense potential not only for genome editing but also for nucleic acid detection, offering highly specific and sensitive diagnostic tools. The primary focus of my dissertation is to engineer …