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Showing 1 to 15 of 15 for “"Myrosinase"”.
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Improving the health benefits of broccoli through myrosinase maintenance
… and a thioglucoside glucohydrolase (myrosinase EC 3.2.1.147). Glucoraphanin and myrosinase are stored separately in the plant cell. When tissue is crushed, such as in insect damage, glucoraphanin and myrosinase come in contact and form an intermediate that breaks down to form the …
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Biochemical and Molecular Approaches to Characterizing the Myrosinase Enzyme System in Horseradish (Armoracia Rusticana)
A full length cDNA (ArM1) encoding myrosinase was cloned from horseradish root by RACE-PCR. ArM1 has an open reading frame of 1,614 nucleotides with a deduced protein of 538 amino acid and molecular mass of 61.6 kDa. Heterologous expression of ArM1 in Spodoptera frugiperda insect cells resulted in …
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Variation and quantitative trait loci analysis of myrosinase activity and phenolic compound accumulation in Brassica oleracea var. italica
Made available in DSpace on 2016-03-02T20:24:12Z (GMT). No. of bitstreams: 2 GARDNER-THESIS-2015.pdf: 3191246 bytes, checksum: 282a4f72b0a6003e1ab58dc6ded758c2 (MD5) LICENSE.txt: 4211 bytes, checksum: 9b8349680069b41d9e16d0ea2a47d366 (MD5) Previous issue date: 2015-12-04
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The glucosinolate/myrosinase system: variation in glucosinolates, hydrolysis products, transcript abundance, and quinone reductase bioactivity in Brassica sp. crops
… data for genes associated with the glucosinolate/myrosinase system in an attempt to predict final phytochemical phenotypes in broccoli. It was found that models built using gene transcript abundance as predictors delivered satisfactory predictive ability for phytochemical phenotypes collected in …
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The bioavailability of sulforaphane from broccoli products in men and its epigenetic activity
… (SF). Sulforaphane is derived from the myrosinase-catalyzed hydrolysis of glucoraphanin. Both glucoraphanin and myrosinase are present in fresh or lightly cooked broccoli and broccoli sprouts. Like myrsoinase, gut microflora are capable of hydrolysis of glucoraphanin to SF, although to a …
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Improving sulforaphane bioavailability from cooked broccoli
… cooked broccoli (CB); unfortunately, the enzyme myrosinase is inactivated by heat, resulting in the failure of glucoraphanin (GRP) hydrolysis to sulforaphane (SF), the potent anti-cancer compound in broccoli. Certain unknown bacteria in the gut microbiota also have myrosinase-like activities that …
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Digestive metabolism of glucosinolates: a novel approach using urinary markers for estimating the release of glucosinolate breakdown products in the gastro-intestinal tract of mammals.
… of glucosinolates by plant or microbial myrosinase yields a range of metabolites including beneficial isothiocyanates. Little is known about the fate of glucosinolates after their ingestion. Using urinary end-products of metabolism as markers, measurement of the production of …
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The Metabolic Fate of Glucoraphanin in F344 Rats
… and are dependent on an endogenous plant enzyme, myrosinase. Most cooking methods inactivate myrosinase. Thus when humans consume cooked broccoli they are dependent on gut microbes to hydrolyze GP to reap the health beneficial effects of SF. Relatively little is known about the hydrolysis products …
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Retention of Nutrients and Phytochemicals in Fresh and Processed Broccoli
… 31.1%, respectively). The initial activity of myrosinase decreased significantly (p $<$ 0.01) during two weeks of storage from 19.2 to 13.3 $\mu$mole min$\sp{-1}$ glucose mg$\sp{-1}$ protein). Microwave cooking (8 min) significantly decreased (p $<$ 0.01) the initial activity of myrosinase to …
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The effect of raw and processed vegetables on colonocyte dna damage.
… is largely dependent on the plant enzyme myrosinase; in contrast, the profile of products formed depends on the parent GLS, conditions of hydrolysis and the presence of a cofactor - epithiospecifier protein (ESP). Thermal processing may deactivate myrosinase and ESP, thereby altering both …
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Microbial glucosinolate metabolism: Characterization of Enterococcus faecalis XL1 and functional analysis of a GH4 enzyme
… and anti-inflammatory properties. When plant myrosinases are inactivated by cooking, gut microbes become the primary contributors to ITC formation. However, the microbial enzymes and pathways responsible for GSL metabolism remain poorly understood. This dissertation investigates the bacterial …
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Improving the Health Benefit of Broccoli by Maximizing Sulforaphane Formation: A Role for Epithiospecifier Protein
… broccoli tissue must be crushed, allowing the myrosinase-catalyzed hydrolysis of the glucosinolate precursor, glucoraphanin. A common assumption has been that sulforaphane is the sole product of glucoraphanin hydrolysis, but several studies have demonstrated that a nitrite analog to …
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Enhancing human health promoting activity through the regulation of the methyl jasmonate mediated glucosinolate biosynthesis in Brassica oleracea
… genes BoCYP79B2, BoCYP83B1, as well as myrosinase, epithiospecifier protein modifier 1 (BoESM1), and epithiospecifier protein (BoESP) genes. Consequently, neoglucobrassicin and gluconasturtiin concentrations were significantly increased by MeJA treatment. In addition, increased …