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Showing 1 to 20 of 100 for “"Myotubes"”.
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Matrix-mediated formation & innervation of skeletal myotubes
The field of tissue engineering utilizes combinations of cells, biomaterials, and therapeutic molecules in order to generate therapies and better understand the emergent behaviors involved in tissue formation and diseases. One of the goals of tissue engineering is to be able to recapitulate the in …
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The Mechanism of Palmitate Decreased Glucose Transpoter 4 Expression in C2 Myotubes
"차 례 국문요약 ⅰ 차례 ⅳ 그림 차례 ⅶ 표 차례 ⅸ Ⅰ. 서론 1 A. 당뇨병 (Diabetes Melitus) 1 B. 제 2형 당뇨병 1 1. 인슐린 저항성 (Insulin Resistance) 4 2. 인슐린 저항성에서 유리지방산의 역할 6 C. 연구목적 9 Ⅱ. 재료 및 방법 10 A. 재료 10 1. 재료 10 B. 방법 14 1. 세포주 및 세포 배양 14 2. Differentiation 14 3. Western blotting 14 4. 2-deoxyglucose uptake 15 5. RT-PCR 15 6. …
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Purification of A Serum Factor That Triggers Cell Cycle Re-entry In Differentiated Newt Myotubes
… re-entry in differentiated multinucleated newt myotubes. The activity is present in serum of all mammalian species tested so far and, interestingly, thrombin proteolysis amplifies the activity from both serum and plasma. We think this serum factor provides a link between wounding and …
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THE EFFECT OF CONTRACTILE ACTIVITY AND SUBSTRATE CHALLENGES ON METABOLIC FLEXIBILITY IN HUMAN PRIMARY MYOTUBES
The skeletal muscle of severely obese individuals (BMI > 40 kg/m²) is characterized by a depressed ability to oxidize fatty acids and a failure to upregulate fatty acid oxidation (FAO) in response to increased lipid availability, both of which may contribute to a positive lipid balance and weight …
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Effects of resveratrol and contractile activity in human skeletal muscle myotubes from severely obese individuals
… investigate this, primary human skeletal muscle myotubes from severely obese subjects (BMI [greater-than or equal to] 40 kg/m2) were isolated from muscle biopsies, and both resveratrol and contractile activity were examined. Specifically, 24 hours of resveratrol treatment (1 [micro]M) improved …
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The Role of Leucine and Its Metabolite (KIC) in Insulin Signalling and Glucose Transport in L6 Myotubes
… other amino acids on glucose transport in L6 rat myotubes. Here we report that leucine significantly suppresses insulin-stimulated glucose uptake in skeletal muscle cells and particularly at 150 M, there is a 75% reduction in insulin-mediated glucose transport (p<0.01). This occurs in parallel …
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Over-expression of NRF-1 in C2C12 myotubes increases GLUT4 content via a transcriptional cascade involving MEF2A
… b) alters MEF2A-MEF2D dimer formation in C2C12 myotubes.
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An investigation into the novel effects of melatonin treatment on differentiated C2C12 myotubes in the absence and presence of pathology
… mitochondrial oxygen consumption and OXPHOS in myotubes by limiting oxygen wastage via an oxygen-sparing action. Moreover, in the pre and curative setting, MEL upregulated mitochondrial fusion. General Methods: For papers 1-3, we have employed an in vitro approach, where differentiated C2C12 …
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Roles of Growth Hormone, Insulin-Like Growth Factor I, and Sh3 and Cysteine Rich Domain 3 in Skeletal Muscle Growth
… proliferate as myoblasts or induced to fuse into myotubes in culture. GH increased protein synthesis without affecting protein degradation in myotubes; GH had no effect on proliferation of myoblasts; GH had no effect on IGF-I mRNA expression in either myoblasts or myotubes. These data suggest that …
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A high-content multiplexed screening platform for the evaluation and manipulation of force and fatigue of adult derived skeletal muscle myotubes in defined serum-free medium
… culture techniques to create functional in vitro myotubes derived from adult skeletal muscle satellite cells. The second was to manipulate the inherent muscle parameters such as force output and fatigue of these myotubes by employing exercise regimes or by small molecule application. The …
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Bmp proteins in urodele myotube cell cycle re-entry and in regeneration
… form these mononucleate cells the differentiated myotubes in vivo must re-enter and complete the cell cycle; they again proliferate and produce progeny. A key question is what factors induce the myotubes to re-enter the cell cycle and proliferate. Early events of cell cycle re-entry can be studied …
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Investigation of a role for the α7 integrin as a mechanotransducer of hypertrophic signaling in skeletal muscle
… from wild type (WT) mice were developed into myotubes in culture to determine the hypertrophic signaling response to mechanical strain. Primary myoblasts from α7 knockout (KO) mice were used as controls for these experiments. Further experimentation employed transient transfection of WT …
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Mechanisms of Ionophore Toxicoses: Laidlomycin Propionate Toxicosis in the Horse and Monensin, Laidlomycin, and Laidlomycin Propionate in Muscle Cultures
… exposure in 3-day (myoblasts) and 14-day ($>$90% myotubes) cultures of E-63 rat skeletal muscle cells. Each of these ionophores induced dose-dependent increases in lactate concentrations, decreases in pyruvate concentrations, and decreases in accumulations of NR. Cultures of both myoblasts and …
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Genes with Physiological Roles in Callipyge Muscle Hypertrophy
… MYH4 luciferase activity. In DLK1-treated myotubes, the mRNA expression of Myh4 and DNTTIP1 were both increased, suggesting DLK1 may regulate Myh4 expression through elevated DNTTIP1 expression. Though PARK7 was not up-regulated in DLK1-treated cells, its increased levels in hypertrophied …
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Positioning Microtissues on the Differentiation Continuum Reveals their Utility for Studying Skeletal Myopathies and Metabolic Disease
… in vitro models utilize two-dimensional (2D) myotubes, these cultures express low levels of insulin-responsive GLUT4 and elevated levels of insulin-independent GLUT1, limiting their relevance for modeling insulin resistance. Human skeletal muscle microtissues, differentiated under uniaxial …
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mTOR Signaling in Cellular Growth and Skeletal Muscle Maturation
… the fusion of myoblasts to form multinucleated myotubes. Skeletal myofibers also undergo hypertrophy with work overload, characterized by size increase and biochemical changes. I showed that the IGF-I-mediated skeletal muscle hypertrophy requires mTOR and its kinase activity, as well as its …
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AMP Deaminase 3 overexpression regulates cellular energetics and signaling for PGC-1α activation.
… energetics (ATP, ADP, AMP). Methods: Myotubes were transduced with adenoviruses encoding AMPD3 or GFP (control) and then treated for 1 h with 0.6mM 2,4 dinitrophenol (DNP). Nucleotides, amino acids, and proteins were extracted immediately after DNP treatment and measured by UPLC and …
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The Role of Neuropeptide Y Y1R in Skeletal Muscle Lipid Metabolism
… Rat L6, mouse C2C12, and human primary myotubes were incubated in 14C palmitate labeled fatty acid oxidation medium containing 80ng/mL, 250ng/mL, and 500ng/mL of NPY and for a three hour period. Experiments were repeated with the addition of 17mg/mL diprotin A to each NPY treatment. …
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Characterization of an in vitro exercise model and the effects of a metabolic endotoxemia on skeletal muscle adaptation to electric pulse stimulation
… electric pulse stimulation (EPS) of human myotubes in vitro would emulate cellular and molecular responses to exercise observed in vivo. To define this model, we applied EPS to human myotubes for varied lengths of time and measured interleukin-6 (Il-6), peroxisome proliferator-activated …
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Microtubule Motors Drive Nuclear Dynamics and Positioning in Developing Skeletal Muscle Cells
… muscle cell development, nuclear movement in myotubes requires microtubules, but the mechanisms involved have not been elucidated. Here, we find that nuclei actively translocate through myotubes. As they translocate, they also rotate in three-dimensions. These movements require an intact …
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