Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Myosins"”.
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DROSOPHILA CLASS-I MYOSINS THAT CAN IMPACT LEFT-RIGHT ASYMMETRY HAVE DISTINCT ATPASE KINETICS
… MgADP affinity and are the first class-I myosins identified as high duty ratio motors. In addition, I participated in a collaboration project unraveling biochemical differences of two smooth muscle gamma actin (ACTG2) mutations that result in human visceral myopathy: R40C and R257C. I …
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Studies on actomyosin crossbridge flexibility using a new single molecule assay.
… the swiveling of rod about a single myosin. Myosins attached through a single crossbridge displayed mostly similar torsional characteristics compared to myosins attached through two crossbridges, which indicates that most of the torsional flexibility resides in the myosin subfragment-2, and …
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Apicomplexan Class XXVII Myosin - A Computational, Biophysical and Cell Biological Characterisation of PfMyoF in Plasmodium falciparum
… proteins for both transmission and virulence. Myosins are ATP-driven mechanoenzymes which either displace or translocate along actin filaments and typically function in a broad range of cellular activities such as membrane trafficking. Very little is known about the precise functions of the …
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Hindrance of the Myosin Power Stroke Posed by the Proximity to the Troponin Complex Identified Using a Novel LRET Fluorescent Nanocircuit
… every 7 actin monomers, hinders adjacently bound myosins to complete their power stroke; whereas myosins bound in between troponin complexes undergo complete power strokes.
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Regulation of Myo5p function by phosphorylation
The Saccharomyces cerevisiae type I myosins Myo3p and Myo5p are involved in endocytosis and in the polarization of the actin cytoskeleton. In vitro, p21-activated kinases (PAKs) can phosphorylate a single serine in the myosin-I head domain, referred to as TEDS site, thereby activating the myosin-I …
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Understanding Actin Dynamics and Its Regulatory Mechanisms in Developing Stereocilia
… with the presence of the unconventional myosins MYO3A/B and MYO15A at stereocilia tips, implicating these motors in generating or stabilizing filaments required for elongation and widening. Focusing on class III myosins, we demonstrate that MYO3A is the predominant paralog in cochlear …
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The Role of UNC-45 in Heart Development and Human Breast Cancer
… as a molecular chaperone of body-wall muscle myosins. In human and other vertebrates, two UNC-45 genes code two UNC-45 protein isoforms: UNC-45A/a and UNC-45B/b. UNC-45A/a is expressed in all cell types, whereas UNC-45B/b only exists in heart and skeletal muscles. This thesis investigated the …
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Single-Molecule Fluorescence Studies of ReAsH and Myosin VI
… to move in a direction opposite that of other myosins. This reverse directionality enables myosin VI to be involved in endocytosis. We investigated its structural and functional mechanisms. First, we discovered the stepping mechanism of myosin VI. By placing fluorescent molecules in two …
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Regulatory mechanisms of MYO6 motor domain activity and tail interactions
… Originating from an ancestral protein, myosins have evolved into many classes with distinct structural domains and mechanochemical properties that contribute to their specialised functions. Myosins of class VI are exceptional, due to their unique reversed directionality towards to …
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GENERATION OF FULL-LENGTH WILD-TYPE GATA4 PROTEIN AND CHARACTERIZATION OF ITS BINDING TO UNC-45 DOMAINS
… as a chaperone for cardiac and skeletal muscle myosins; regulating their folding, assembly into thick filaments, interaction with other sarcomeric proteins, and degradation. GATA4 is an important transcription factor that regulates the expression of several cardiac muscle proteins, including …
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The UNC-45 molecular chaperone: Its interactions with myosin and its thermosensing properties
… which require assistance of chaperones are myosins. These are motor proteins responsible together with actin for muscle contraction process. The UNC-45 protein is a chaperone that has been identified to be involved during the myosin folding process. During my thesis research, I have …
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Funktionelle Charakterisierung des Vasodilatator stimulierten Phosphoproteins (VASP) für die Stabilität des Aktin-Zytoskeletts und die Integrin-abhängige Zelladhäsion
… und die Phosphorylierung der leichten Kette des Myosins erhöht, was auf ein stabileres und stärker kontrahiertes Aktin- Zytoskelett in diesen Zellen schließen lässt. Die Regulation und Organisation des Aktin- Zytoskeletts beeinflusst auch die zelluläre Adhäsion, die in VASP-defizienten Zellen …
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Aktinabhängige Bewegung und Vererbung von Mitochondrien in Saccharomyces cerevisiae
… Mal die wichtige und direkte Beteiligung eines Myosins an der mitochondrialen Bewegung und Vererbung in S. cerevisiae. Darüber hinaus wurden Mdm31 und Mdm32, zwei funktionell unabhängige Untereinheiten zweier Komplexe der mitochondrialen Innenmembran (IM), als notwendige Komponenten der …
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Myosin 10 is Required for Spinal Motor Axon Growth and Guidance in Zebrafish Embryos
… molecules play a vital role in axon guidance. Myosins are a class of actin binding proteins. <em>Myosin 10</em> (<em>myo10</em>) is a myosin that is highly localized in growth cones indicating their potential role in axon guidance and growth. While <em>myo10</em> has been shown to be involved …
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Pax3 expression in satellite cells of avian skeletal muscle spindles during normal development and with experimental muscle overload
… they maintain small diameters, developmental myosins, Myf5 expression and high SC concentrations. This thesis tests the hypotheses that Pax3 expression is preferentially maintained in SCs of adult skeletal muscle spindles and can be augmented under conditions of SC activation. To study Pax3 …
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Interactions between the plant Golgi apparatus and the cytoskeleton
… on actin filaments and movement is powered by myosins. The hypothesis that actin filaments slide one of top of the other, and drag the organelles along, was tested using the FRAP technique. Kinesin-13a is the only microtubule motor protein localized on Golgi bodies by immunochemical studies. …
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Single-molecule studies of unconventional motor protein myosin VI
… to its distinct features as compared to other myosins, such as its opposite directionality and a much larger step size than expected given the length of its “leg”. This dissertation presents the author‟s graduate work of several single-molecule studies on myosin VI. Special attention was paid …
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Molekulargenetische Analyse der Central Core Disease
… mit dem MYH7-Gen für die beta-Kette des schweren Myosins auf Chromosom 14 vorgeschlagen, dies konnte aber weder bestätigt noch ausgeschlossen werden. Um die Rolle des RYR1-Gens für die CCD zu eruieren wurden die häufigsten bisher bei MHS-Familien gefundenen RYR1-Mutationen an CCD-Patienten …
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Biochemical purification and functional characterization of the She RNP complex from S. cerevisiae
… encodes a member of the class V unconventional myosins. The finding that a motor protein is required for ASH1 mRNA targeting suggested a cytoskeleton-based, active transport mechanism. Functional characterization of the SHE genes in turn established a working model for the 'core She machinery' …
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The Molecular Basis of Tissue Elasticity and Force Balance During Drosophila Gastrulation
The mechanics of folding any material rely on two things: the physical forces forming the fold and the material properties of the substance being folded. When working in biological tissue such as an early Drosophila embryo, there is no existing way to directly measure morphogenetic forces, and the …
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