Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Myosin-Light-Chain Kinase"”.
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Characterization of Smooth Muscle Myosin Light Chain Kinase Using Crystallization and Transient Kinetics
… the first 75 amino acid segment of smooth muscle myosin light chain kinase (MLCK N1-75). As of now, the exact mechanism by which MLCK interacts with actomyosin complexes in smooth muscle is not well-understood because there is no highresolution 3-dimensional structure of MLCK available. In this …
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Role of Myosin Light Chain Kinase in Binge Ethanol and Burn Injury-Induced Intestinal Barrier Dysfunction
… insult alone. Long (210 kDa) smooth muscle myosin light chain kinase (MLCK), an enzyme important for epithelial tight junction maintenance, has been implicated in barrier alterations after burn injury or ethanol exposure alone. With this knowledge, <underline>we hypothesize that the …
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Interactions of Smooth Muscle Myosin Filaments with Actin and Myosin Light Chain Kinase: Insights into the Mechanics of Smooth Muscle Contraction
The functional form of smooth muscle myosin (SMM) within cells is the filament. Smooth muscle contraction is thick (myosin) filament-regulated by phosphorylation of the myosin regulatory light chain (RLC). Upon phosphorylation of Serine 19 of the RLC of SMM by myosin light chain kinase (MLCK), SMM …
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MATHEMATICAL MODELLING OF CALCIUM DEPENDENT ACTIVE CONTRACTION IN A GASTROINTESTINAL SMOOTH MUSCLE CELL
… triggered by calcium dependent activation of Myosin Light Chain Kinase (MLCK) which is the primary mechanism has been described in terms of two interacting modules - the first module describing the activation of MLCK, and the second describing cross-bridge formation between actin and myosin. …
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The influence of myosin regulatory light chain phosphorylation on the contractile performance of fatigued mammalian skeletal muscle
ABSTRACT The myosm regulatory light chain (RLC) of type II fibres is phosphorylated by Ca2+ -calmodulin dependent myosin light chain kinase (skMLCK) during muscular activation. The purpose of this study was to explore the effect of skMLCK gene ablation on the fatigability of mouse skeletal muscles …
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Spatiotemporal organization, regulation and function of traction during neutrophil chemotaxis
… tractions depend upon the activity of non-muscle myosin IIA. Specifically, traction development at the leading edge requires myosin light chain kinase (MLCK)-mediated myosin II contractility and is necessary for α5β1-integrin activation and leading edge adhesion. Localized myosin II activation …
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Force potentiation in the MDX mouse
… PTP changes, fiber typing and Western blots for myosin light chain kinase (MLCK) were performed. YM and YC had similar fiber type profiles (2% I, 58% IIX/D and 40% IIB). In accordance with literature but contrary to expected conditions for elevated PTP, OM had a slower fiber type profile (1.7% I, …
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Regulation of the myogenic response and stretch-induced calcium signaling in the vascular wall: Novel insights into the role of microRNAs and protein tyrosine kinase 2
… novel roles of microRNAs and protein tyrosine kinase 2 in pressure/stretch-induced signaling mechanisms in the vascular wall. Using two different knockout mouse models, we uncovered a novel role of microRNAs in the pressure-induced myogenic response. We demonstrated that global deletion of …
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Epigenetic Modification of Phenotype in Intestinal Smooth Muscle Cells
… increased expression of muscarinic receptor 3, myosin light chain kinase 2, and showed an increased response to carbachol stimulation. TSA and AZA treatment also increased GDNF expression, and improved the neurotrophic function of repeated passage ISMC. Compared to low passage ISMC, repeated …
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The Role of Calcium in Signal Transduction: Kinetics and Regulation of Intracellular Calcium During the Photomechanical Response of the Albino Rat Sphincter Pupillae Smooth Muscle
… → calcium-calmodulin complexes → myosin light chain kinase → phosphorylated myosin → force. In the isolated vertebrate sphincter pupillae, the myosin phosphorylation cascade can be activated very precisely by controlled light stimuli, which result in equivalently precise …
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Activation of smooth muscle and theories of contraction
… the disparity in the number of crossbridges vs. myosin light chain kinase molecules, suggests that models that recognize this disparity would involve a large number of states. These models could have a higher ATP turnover than four state models, but at a cost of requiring much increased …
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Differentially expressed genes in aortic cells from atherosclerosis-resistant and atherosclerosis-susceptible pigeons
… oxidative phosphorylation to produce energy. Myosin light chain kinase and alpha actin were exclusively expressed by the SR SMC, whereas beta actin and collagen were dominant in the WC. Because of the compressed in vitro time frame compared with in vivo development, it was not obvious whether …
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Endothelin-1 and Hypoxic Vascular Remodeling in Ovine Fetal Cerebral Arteries
… coupling of these ligands to the intracellular kinases. Endothelin-1 (ET-1) is an established vasoconstrictor that can activate ETA or ETB receptors, respectively stimulating vasoconstriction and vasodilation. ET-1 activates pathways such as Protein Kinase C (PKC), Ca2+/Calmodulin-Dependent …
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Serum response factor-dependent regulation of smooth muscle gene transcription
… SRF regulates the expression of smooth muscle myosin light chain kinase (smMLCK) to mediate changes in contractility. Using a combination of transgenic reporter mouse and knockout mouse models I demonstrated that a CArG element in intron 15 of the mylk1 gene is necessary for maximal …
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Force potentiation as a modulator of contractile performance: Implications for control of skeletal muscle force and energetics of work
… fast hindlimb muscles from wildtype and skeletal myosin light chain kinase knockout (skMLCK-/-) mice that does not express the primary mechanism for PTP: myosin regulatory light chain (RLC) phosphorylation. In contrast to smooth/cardiac muscles where RLC phosphorylation is indispensable, its …
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Structure, function, and dynamics of cell motility proteins
… observed in the complex of calmodulin with myosin-light-chain-kinase. (3) In MD simulation, water molecules diffuse into the buried nucleotide binding site of the cytoskeletal ATPase actin along two distinct pathways. Of particular interest is the ""back door"" diffusion pathway which is …
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Molecular Mechanism of Zipper Interacting Protein Kinase
… of two enzymes; calcium/calmodulin regulated myosin light chain kinase (MLCK) and smooth muscle myosin phosphatase (SMPP-1M) determine the amount of force generated by smooth muscle. The calcium-independent signaling mediated by myosin phosphatase is regulated by several kinases which include …
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Application of a Novel Quasi-3D Microscopy Technique to Investigate Early Osteocyte Mechanotransduction Events
… where [Ca+2]i oscillations activate phasic actomyosin contractions using a smooth muscle-like mechanism. Fluid shear, ATP, and ionomycin induced [Ca+2]i signaling with a subsequent compression and recovery in actin strains of the cell, being most apparent in the height direction strain. This …
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Purification and characterization of a smooth muscle myosin phosphatase from turkey gizzards
… the reversible phosphorylation of the 20,000 Da myosin light chains (MLC20). The phosphorylation reaction, which is catalyzed by myosin light chain kinase and results in contraction, has been well characterized. In contrast, little is known about the dephosphorylation reaction or the myosin …
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