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Showing 1 to 16 of 16 for “"Myosin V"”.
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Regulation of Myosin V-Cargo Interactions by Phosphorylation
Myosin V is required for the proper transport and localization of specific organelles along actin filaments in many cell types. Two independent kinases, Calcium/calmodulin-dependent kinase II (CaMKII) and p21-activated kinase-1 (PAK1), are able to phosphorylate the globular tail of myosin V. …
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Chemomechanical coupling and motor cycles of the molecular motor myosin V
… for the energy conversion by the molecular motor myosin V, using many different aspects of theoretical physics. Myosin V has been studied extensively in both bulk and single molecule experiments. Its stepping velocity has been characterized as a function of external control parameters such as …
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Regulation of Organelle Transport by Myosin v in the Cell Cycle
… dynein and kinesin II, and the actin motor myosin V. Here, the cell cycle regulation of myosin V was investigated. In earlier work from our lab, myosin V was found to be downregulated, as it was released from organelles during mitosis [Rogers et al., J Cell Biol. 146, 1265 (1999)]. This …
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Lighting up life : fluorescence studies of the Shaker K⁺ channel and myosin V in action
Submitted by Meng Tao (mengtao2@illinois.edu) on 2012-11-14T18:31:06Z No. of bitstreams: 1 Snyder_G.pdf: 2503154 bytes, checksum: f5a1b46abcce638cc410d021a34eed05 (MD5)
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Lighting Up Life: Fluorescence Studies of the Shaker Potassium Ion Channel and Myosin v in Action
"The other protein I studied is myosin V which walks bipedally along actin filaments taking steps which displace the center of mass 37 nm per step. A controversy over the nature of this movement was settled earlier this year by other members of our laboratory when they showed that myosin V …
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Signals Regulating Organelle Transport in Melanophores
… organelles were examined. Immunoprecipitation of myosin V showed that the motor subunit is more heavily phosphorylated in cells aggregating pigment compared to cells dispersing pigment. Furthermore, levels of myosin V on melanosomes are higher in cells dispersing pigment. Therefore the association …
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Mechanisms and Regulation of Organelle Transport in Xenopus Melanophores
… were also found to possess the actin-based motor myosin-V and to move on actin filaments in a cell-free system. Using Xenopus egg extracts arrested in interphase or metaphase, we also demonstrated that actin-based melanosome motility is regulated in a cell cycle-dependent manner. In metaphase …
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Studying Processive Molecular Motors Inside Live Cells
… steps of the processive molecular motors myosin V, kinesin and myosin VI, in the order. In our study we have searched for the possibilities of working on the same motor proteins in vivo, in other words, inside live cells. In order to overcome the complications of imaging in live organisms …
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Fluorescence Imaging With One Nanometer Accuracy: Determination of How Processive Motors Walk
… without dissociating from the cytoskeleton. Myosin V, myosin VI, and kinesin are well known processive motors. Structurally, these motors are dimeric having two motor heads, two legs, and a common stalk. Whether they move by a ""hand-over-hand"" or an ""inchworm"" model has been a …
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Directed transport without net bias in physics and biology
… bias. The processive molecular motors, of which myosin-V and kinesin are the most well known, 'walk' along the cytoskeleton, the internal skeleton of the cell. Both myosin-V and kinesin are known to walk in a 'hand over hand' manner, fuelled by ATP, adenosine triphosphate. Unresolved questions …
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Directed transport without net bias in physics and biology
… bias. The processive molecular motors, of which myosin-V and kinesin are the most well known, 'walk' along the cytoskeleton, the internal skeleton of the cell. Both myosin-V and kinesin are known to walk in a 'hand over hand' manner, fuelled by ATP, adenosine triphosphate. Unresolved questions …
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Biomimetic cytoskeleton assemblies and living cells on micropillar force sensor arrays
… divalent cations and with fluorescently labelled myosin II motors. The mechanical properties of single filaments are determined by Fourier analysis of their fluctuations. Transport properties of myosin V motors on the networks are quantified. Microtubule gliding assays in a three dimensional …
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Rapid Two-Photon Holographic Scan Microscopy with Individual Quantum Dots and Its Applications in Biological Samples
… was used to resolve the step size of individual Myosin V motors in vitro, indicating the 2-photon field does not damage the ATPase of myosin. We validated the helical spatial arrangement of LamB receptors on live E. coli cells with the 2-photon fast hologram scan microscopy. The endocytosis of …
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Regulation of Bidirectional Microtubule-Dependent Organelle Transport in Xenopus Laevis Melanophores
… proteins kinesin-2, cytoplasmic dynein, and myosin V. While the motor proteins responsible for melanosome transport have been identified, the regulatory mechanisms that control melanosome transport are less known. The following dissertation seeks to shed light on the mechanisms that regulate …
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Myosin structural dynamics: mechanistic insights and therapeutic technology developments
… protein in this contractile apparatus is myosin. Our lab has developed a strategic tool called transient time-resolved FRET, (TR)2FRET, to measure directly, with sub-nanometer and sub-millisecond resolution, the structural and biochemical kinetics of muscle myosin. This tool allows us to …
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Identification of components required for mitophagy and mitochondrial inheritance by genetic screens in yeast
… transported along the actin cytoskeleton by the myosin V motor protein Myo2. The mutant myo2(LQ) allele carries two amino acid substitutions resulting in impaired mitochondrial motility. By introducing this allele into yeast deletion mutants on a genome-wide scale by synthetic genetic array …