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Showing 1 to 20 of 50 for “"Myosin Light Chain"”.
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Characterization of Smooth Muscle Myosin Light Chain Kinase Using Crystallization and Transient Kinetics
… the first 75 amino acid segment of smooth muscle myosin light chain kinase (MLCK N1-75). As of now, the exact mechanism by which MLCK interacts with actomyosin complexes in smooth muscle is not well-understood because there is no highresolution 3-dimensional structure of MLCK available. In this …
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Role of Myosin Light Chain Kinase in Binge Ethanol and Burn Injury-Induced Intestinal Barrier Dysfunction
… insult alone. Long (210 kDa) smooth muscle myosin light chain kinase (MLCK), an enzyme important for epithelial tight junction maintenance, has been implicated in barrier alterations after burn injury or ethanol exposure alone. With this knowledge, <underline>we hypothesize that the …
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Interactions of Smooth Muscle Myosin Filaments with Actin and Myosin Light Chain Kinase: Insights into the Mechanics of Smooth Muscle Contraction
The functional form of smooth muscle myosin (SMM) within cells is the filament. Smooth muscle contraction is thick (myosin) filament-regulated by phosphorylation of the myosin regulatory light chain (RLC). Upon phosphorylation of Serine 19 of the RLC of SMM by myosin light chain kinase (MLCK), SMM …
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MATHEMATICAL MODELLING OF CALCIUM DEPENDENT ACTIVE CONTRACTION IN A GASTROINTESTINAL SMOOTH MUSCLE CELL
… triggered by calcium dependent activation of Myosin Light Chain Kinase (MLCK) which is the primary mechanism has been described in terms of two interacting modules - the first module describing the activation of MLCK, and the second describing cross-bridge formation between actin and myosin. …
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Stretch-Dependant Tonic Force Maintenance in Rabbit Epigastric Artery
… of hypertension. Upon muscle stimulation, Ca2+, myosin light chain (MLC) phosphorylation, crossbridge cycling rates and force increase to high levels, but with time, force is maintained while all other parameters of muscle activation fall to low levels. Thus, contraction is divided temporally …
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The influence of myosin regulatory light chain phosphorylation on the contractile performance of fatigued mammalian skeletal muscle
ABSTRACT The myosm regulatory light chain (RLC) of type II fibres is phosphorylated by Ca2+ -calmodulin dependent myosin light chain kinase (skMLCK) during muscular activation. The purpose of this study was to explore the effect of skMLCK gene ablation on the fatigability of mouse skeletal muscles …
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Analysis of the role of extracellular signal regulated kinase (ERK5) in the differentiation of muscle cells
… genes, such as the cdk-inhibitor p21 gene, the myosin light chain (MLC1A) gene, or an E-box containing promoter element, where myogenic basic-helix-loop-helix proteins such as MyoD or myogenin bind. Moreover, myogenic differentiation is completely blocked, when ERK5 expression is inhibited by …
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The Src- and Abl- Dependent Regulation of Net1A In Breast Cancer
… potentiates EGF-stimulated RhoA activation, Myosin Light Chain 2 phosphorylation, and F-actin accumulation. Expression of Net1A Y373D in breast cancer cells significantly increases cell motility and Matrigel invasion. Moreover, Net1A is required for Abl1- stimulated cell motility, which is …
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K+-Induced Smooth Muscle Calcium Sensitization Requires RhoA Kinase (ROK) Translocation to Caveolae Which Is Inhibited in Non-Neuronal Cell Memory
… did inhibit KCl-induced tonic (5’) force and myosin light chain (MLC) phosphorylation in rabbit artery. Y-27632 also inhibited Bay K-8644- and ionomycin-induced MLC phosphorylation and force, but did not inhibit KCl-induced calcium entry or peak (~15”) force. Moreover, KC1 and Bay K-8644 …
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Spatiotemporal organization, regulation and function of traction during neutrophil chemotaxis
… tractions depend upon the activity of non-muscle myosin IIA. Specifically, traction development at the leading edge requires myosin light chain kinase (MLCK)-mediated myosin II contractility and is necessary for α5β1-integrin activation and leading edge adhesion. Localized myosin II activation …
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Characterization of zebrafish zipper-interacting protein kinase
… Kinase (ZIPK) is a known modulator of actin-myosin contractility in vertebrate species. Interestingly, rodent and mouse ZIPK has undergone a divergence in regulation in comparison to other vertebrate orthologs including human. Whereas the human ortholog of ZIPK requires phosphorylation of …
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Evidence for Absence of Latchbridge Formation in Phasic Saphenous Artery
… Reduced tonic stress correlated with reduced myosin light chain (MLC) phosphorylation in SA (28% vs. 42% in FA). SA expressed more MLC phosphatase than FA, and permeabilized (β-escin) SA relaxed more rapidly than FA in the presence of MLC kinase blockade, suggesting that MLC phosphatase …
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Regulation of blood platelet function by nitric oxide
… shape change is driven by phosphorylation of myosin light chain (MLC) and the experimental data shows that NO blocked this critical phosphorylation event. Phospho-MLC generated in response to platelet agonists occurs through a Ca²+ dependent and RhoA kinase (ROCK)-dependent mechanisms and NO …
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Force potentiation in the MDX mouse
… PTP changes, fiber typing and Western blots for myosin light chain kinase (MLCK) were performed. YM and YC had similar fiber type profiles (2% I, 58% IIX/D and 40% IIB). In accordance with literature but contrary to expected conditions for elevated PTP, OM had a slower fiber type profile (1.7% I, …
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The Regulation of Phosphorylation Events in Platelets
… studies have established the importance for myosin kinase in its role for phosphorylating the myosin light chain (MLC) downstream of ADP receptors. These studies have shown MLC phosphorylation occurs rapidly and is essential for shape change following the stimulation of ADP receptors. …
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Chemical Biology Tools for Biochemical and Functional Analysis of Myosin Phosphatase
… hypocontractility in AF to dysregulation of myosin light chain phosphatase (MLCP), a holoenzyme composed of a catalytic subunit (PP1cβ) and a regulatory subunit that targets the phosphatase to sarcomeric substrates. PPP1R12C is an atrial-enriched MLCP regulatory subunit that directs PP1cβ to …
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Characterization of the zebrafish zipper interacting protein kinase homolog
… affect cell shape through changes in the actomyosin cytoskeleton resulting in aberrant cytoskeletal structures. Finally, we have also shown through phosphorylation assays that ZIPK phosphorylates and thus regulates MYPT-1, a scaffolding protein of the myosin protein phosphate complex and …
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Developmental regulation of smooth muscle contraction in the urinary bladder
… extracellular calcium concentrations. Non-muscle myosin is contributing to the force development in the newborn but is not the cause for the increased calcium sensitivity. The increased calcium sensitivity is due to a lower phosphatase activity and a lower expression of the myosin light chain …
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