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Showing 1 to 20 of 356 for “"Myosin"”.
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Studies of myosin mechanics using fluorescence
… to the interaction. Molecular motors such as myosin provide an ideal system for fluorescence. The multitude of currently available fluorescent techniques allow for the study of each important step required for myosin to generate directed motion along an actin filament. Several fluorescent …
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Myosin and electrophysiological heterogeneity in cardiac muscle
Abstract available p. v-vii
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Funktionelle Charakterisierung von Myosin IK in Dictyostelium discoideum
… Amöbe D. discoideum exprimiert 12 verschiedene Myosinisoformen, von denen sieben der Klasse I angehören. Myosin IK, ein Mitglied dieser Klasse, hat eine einzigartige Struktur. Es zeichnet sich durch eine überdimensionale Motordomäne und sehr kurzen Halsregion aus. Die Schwanzdomäne fehlt …
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Regulation of Myosin V-Cargo Interactions by Phosphorylation
Myosin V is required for the proper transport and localization of specific organelles along actin filaments in many cell types. Two independent kinases, Calcium/calmodulin-dependent kinase II (CaMKII) and p21-activated kinase-1 (PAK1), are able to phosphorylate the globular tail of myosin V. …
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Interactions of Smooth Muscle Myosin Filaments with Actin and Myosin Light Chain Kinase: Insights into the Mechanics of Smooth Muscle Contraction
The functional form of smooth muscle myosin (SMM) within cells is the filament. Smooth muscle contraction is thick (myosin) filament-regulated by phosphorylation of the myosin regulatory light chain (RLC). Upon phosphorylation of Serine 19 of the RLC of SMM by myosin light chain kinase (MLCK), SMM …
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The effects of force on actin-myosin ATPase activity
… they generate movement through the actin-myosin ATPase reaction. However, the chemical reaction underlying movement remains unclear. Specifically, little is known about how the forces generated by muscle influence the chemistry of the force-generating reaction. We measured the effects of …
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Myosin structural dynamics: mechanistic insights and therapeutic technology developments
… protein in this contractile apparatus is myosin. Our lab has developed a strategic tool called transient time-resolved FRET, (TR)2FRET, to measure directly, with sub-nanometer and sub-millisecond resolution, the structural and biochemical kinetics of muscle myosin. This tool allows us to …
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Single-Molecule Fluorescence Studies of ReAsH and Myosin VI
… proteins. We also have used FIONA to study myosin VI, the only myosin to move in a direction opposite that of other myosins. This reverse directionality enables myosin VI to be involved in endocytosis. We investigated its structural and functional mechanisms. First, we discovered the …
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Single-molecule studies of unconventional motor protein myosin VI
Myosin VI is one of the myosin superfamily members that are actin-based molecular motors. It has received special attention due to its distinct features as compared to other myosins, such as its opposite directionality and a much larger step size than expected given the length of its “leg”. This …
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Mechanisms of myosin regulation and function during tissue folding
… by cycles of assembly and disassembly of actin-myosin networks at the cell apex, called pulses. Pulsatile myosin leads to phases of cellular contraction and cell shape stabilization that result in step-wise apical constriction. While many of the key components of the pathway have been …
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Structure and Dynamics of the Myosin Dimer Using Fluorescence Techniques
Lastly, I present preliminary results on the development of a new technique called two-color FRET-FCS (a combination of Fluorescence Resonance Energy Transfer and Fluorescence Correlation Spectroscopy) and its application to the dynamical study of a model DNA hairpin. This technique can ultimately …
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Purification of Two Myosin -Degrading Proteases From Porcine Skeletal Muscle
Myosin is the major skeletal muscle protein. However, the protease system responsible for myosin degradation in vivo has not yet been clearly described. Two myosin degrading proteases, a serine protease and a cysteine protease that might have some function in myosin degradation, were isolated from …
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Chemical Biology Tools for Biochemical and Functional Analysis of Myosin Phosphatase
… hypocontractility in AF to dysregulation of myosin light chain phosphatase (MLCP), a holoenzyme composed of a catalytic subunit (PP1cβ) and a regulatory subunit that targets the phosphatase to sarcomeric substrates. PPP1R12C is an atrial-enriched MLCP regulatory subunit that directs PP1cβ to …
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Conformational Studies of Myosin and Actin with Calibrated Resonance Energy Transfer
… to study the conformational changes of actomyosin during ATP hydrolysis. To calibrate the technique, the parameters for resonance energy transfer were defined. With conformational searching algorithms to predict probe orientation, the distances measured by resonance energy transfer are …
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Chemomechanical coupling and motor cycles of the molecular motor myosin V
… for the energy conversion by the molecular motor myosin V, using many different aspects of theoretical physics. Myosin V has been studied extensively in both bulk and single molecule experiments. Its stepping velocity has been characterized as a function of external control parameters such as …
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Understanding the ATP hydrolysis mechanism in myosin using computer simulation techniques
… into mechanical work. A prominent example is myosin which drives muscle contraction and a large number of additional cellular transport phenomena in all living organisms. While hydrolyzing ATP, myosin translocates along an actin filament. The catalytic cycle for ATP hydrolysis and the …
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Regulation of Organelle Transport by Myosin v in the Cell Cycle
… dynein and kinesin II, and the actin motor myosin V. Here, the cell cycle regulation of myosin V was investigated. In earlier work from our lab, myosin V was found to be downregulated, as it was released from organelles during mitosis [Rogers et al., J Cell Biol. 146, 1265 (1999)]. This …
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The Myosin-Binding UCS Domain but not the Hsp90-Binding TPR Domain of the UNC-45 Chaperone is Essential for Myosin Accumulation and Assembly in Caenorhabditis elegans
… and A band assembly. We show that the myosin-binding UCS domain of UNC-45 alone is sufficient to rescue embryonic lethal unc-45 null mutants arrested in embryonic muscle development and temperature-sensitive loss-of-function unc-45 mutants defective in worm A band assembly. Removal of …
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