Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 64 for “"Mycobacterium smegmatis"”.
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Glyoxylate Metabolism in Mycobacterium smegmatis
<p>Much has been learned about Mycobacterium tuberculosis, the causative agent of tuberculosis, "the great white plague," since the bacterium was isolated and initially characterized by Robert Koch over a century ago. Over the last decade, new genetic tools for manipulation of the bacterium have …
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Analysis of the multiple chaperonins of Mycobacterium smegmatis
… two or more cpn60 genes. The non-pathogenic Mycobacterium smegmatis has three cpn60 homologues, while the pathogenic Mycobacterium tuberculosis has two. This study is a functional characterisation of the chaperonins of M. smegmatis. Expression of cpn60.1, cpn60.2 and cpn10, but not cpn60.3, …
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High-Throughput Determination of Mycobacterium smegmatis Protein Complex Structures
… The rise of multiand extensively-drug resistant Mycobacterium tuberculosis (Mtb), the causative agent of TB, has led to further developments in understanding the physiology of Mtb during infection, as well as searching for novel drug targets, in order to combat the disease. Our understanding of …
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Characterisation of the cold-shock response in Mycobacterium smegmatis
The response of Mycobacterium smegmatis to a cold shock was investigated in order to gain insight into the stress responses of members of the genus Mycobacterium. Mycobacterium smegmatis cultures were shocked from 37°C to 30°C, 25°C, 15°C, and 10°C and the effects on both growth (ATP concentration, …
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Helical reconstruction of Mycobacterium smegmatis Mycothiol S-conjugate amidase filaments
… several antibiotics for mycobacteria, including Mycobacterium tuberculosis. The central enzyme used in the clearance of electrophilic toxins is Mycothiol S-conjugate amidase (Mca). Mca is similar to a biosynthetic enzyme MshB, which has partial overlapping substrate activity and is the closest …
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Characterization of protein secretion in Mycobacterium leprae using phoA fusions in Escherichia coli and Mycobacterium smegmatis
… using surrogate host bacteria, E. coli and M. smegmatis. Bioinformatics identified 24 proteins with high probability for secretion in M. leprae. Fifteen of 24 ML genes showed more than 50% amino acid homology with their M. tuberculosis counterparts and were studied for gene expression and …
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Targeted depletion of RibF, a putative bifunctional FAD synthetase/ flavokinase in Mycobacterium smegmatis using CRISPR interference
… shorter regimens to treat the causative agent, Mycobacterium tuberculosis, an imperative which demands the identification of new drug targets in essential mycobacterial pathways. To that end, the work presented in this dissertation aimed to functionally characterize ribF, an essential gene in …
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Characterisation of dysregulated proteins in macrophages infected with Mycobacterium smegmatis focusing on matrix metalloproteases and their effectors
… is a key facilitator in transmission of Mycobacterium tuberculosis (M. tb). Upregulation of matrix metalloproteases (MMPs) has been documented in patients with tuberculosis (TB), while their tissue inhibitor (TIMPs) levels remained the same. Animals which can develop cavities have …
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Differential Lipidomic and Proteomic Responses Induced by Sub-lethal Drug Challenge in Susceptible and Drug Resistant Mycobacterium smegmatis
… rifampicin exposure upon the model organism Mycobacterium smegmatis over time and determine what phenotypic adaptations might be observed and explained by alterations in the proteome and lipidome, with special focus on the cell wall sub-proteome. From these data we formed several new …
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The Mycobacterium smegmatis "Proteome" : effects of growth phase on total protein synthesis and on the response to heat shock
… stationary phase, the effect of growth phase of Mycobacterium smegmatis on total protein synthesis and on the heat shock response was investigated. De novo protein synthesis was monitored by labelling with 35 [S]methionine and the protein expression profiles analysed using one- …
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Generation of <i>Mycobacterium smegmatis</i> Model Systems for Testing Inhibitors of the Mycobactin Siderophore of <i>Mycobacterium tuberculosis</i>
<p><em>Mycobacterium tuberculosis (Mtb)</em> is the pathogenic bacterium causative of tuberculosis. According to the 2021 World Health Organization (WHO) Global Tuberculosis (TB) Report, there were approximately 5.8 million TB cases in 2020, and 1.3 million TB deaths among HIV negative people in …
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Atividade bactericida da ß-lapachona, isoniazida, de um derivado 1,2,4-oxadiazol-hidrazida e de suas associações frente ao Mycobacterium fortuitum e Mycobacterium smegmatis
… e suas associações frente ao M. fortuitum e M. smegmatis utilizando como métodos a Concentração Inibitória Mínima (CIM) e a Cinética Bactericida. A CIM permitiu determinar a menor concentração das drogas capaz de inibir o crescimento visível do microrganismo. Através da curva de morte versus …
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Conditions affecting ergothioneine levels in Mycobacterium smegmatis & the attempted isolation of α-N, N, N-Histidine methyltransferase, the first enzyme in ergothioneine biosynthesis
Ergothioneine and mycothiol are the two major low molecular weight thiols present in mycobacteria. The generation of mycothiol-deficient mutants has demonstrated its role in protecting M tuberculosis against oxidative and nitrosative stress. To date, no ergothioneine-deficient mutants have been …
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Prevalence of the gene lsr2 among the genus Mycobacterium and an investigation into changes in biofilm formation when inactivating lsr2 regulated genes in Mycobacterium smegmatis
Mycobacterium smegmatis is a non-pathogenic model organism commonly used to study the human pathogen Mycobacterium tuberculosis. M. smegmatis has a rough colony morphology and is capable of forming biofilms, however, when the DNA-regulating protein Lsr2 is inactivated the bacteria forms a smooth …
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Proteomics of physiologically relevant stresses on model mycobacterium tuberculosis organisms, and the effects on virulence
… and it is estimated that 1.5 million died. Mycobacterium tuberculosis which is the causative agent of tuberculosis disease, is an obligate aerobic bacterium with a unique cell wall and is capable of surviving and infecting a immunocompetent host. The bacteria are spread by aerosols, where an …
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Isolation of bacteriophages against Streptococcus species; Lysogeny of Ten Mycobacteriophages for host Mycobacterium tuberculosis H37Ra
… specifically, those that infect Mycobacterium smegmatis mc2155. Two projects are presented in this thesis. The first is the methods to capture and isolate Streptococcus bacteriophages using human saliva tested against Streptococcus mutans lab strains, and other wild-type bacteria …
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An assessment of Hypoxis hemerocallidea extracts, and actives as natural antibiotic, and immune modulation phytotherapies
… a South African medicinal plant, using Mycobacterium smegmatis.
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Replication fidelity in the mircroevolution of mycobacteria
… of targeted amino acids, resulting in a panel of Mycobacterium smegmatis mutants carrying selected dnaE1 alleles. A complementary approach investigated the contribution of the mycobacterial proofreading DnaQ subunit homolog to the maintenance of DnaE1-dependent replicative fidelity by generating a …
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Systematic Analysis of Gene Toxicity in Mycobacteriophage Bromden
… when expressed individually in its host, Mycobacterium smegmatis. To perform this screen, I created a plasmid library that allows the inducible expression of each Bromden gene. I found 42 genes affecting the growth of the host bacteria which will be pursued in the laboratory to understand …
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