Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 182 for “"Mycobacterial"”.
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Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector
This study aimed to investigate whether the mycobacterial replicon may also play a role. The common mycobacterial replicon in episomal E. coli-mycobacterial shuttle vectors contains the truncated rap gene, encoding an auxiliary replication factor, and the repA and repB genes which code for …
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Visualising the Mycobacterial Mutasome
… Previous work has established that this “mycobacterial mutasome” comprises the specialist DNA polymerase, DnaE2, and accessory factors of unknown function, ImuA′ and ImuB. However, the molecular interactions and sub-cellular recruitment dynamics enabling mutasome function remain poorly …
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Inquiries into the Mycobacterial Caseinolytic Protease System
… in conjunction with our laboratory's work on mycobacterial genetic vulnerability, identified the caseinolytic protease (Clp) system, a central component of the proteostasis network, as essential and highly vulnerable in Mtb. As an attractive, emergent anti-tubercular target, a deeper …
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Functional and structural characterisation of Mycobacterial Chaperonins
… double ring structure of 14 subunits, the Mycobacterial chaperonins are very unstable and appear to form much smaller complexes. Given that the large structures formed by most chaperonins are vital to their mechanism of action, it is unclear why the oligomers are so much less stable in …
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N-Glycosylation Protects Host from Mycobacterial Infection
… are required for the macrophage to resist mycobacterial infections. N-glycosylation attaches an oligosaccharide molecule to nascent secretory or membrane proteins within the endoplasmic reticulum (ER) for protein folding and trafficking. Defects of N-glycosylation induce the unfolded …
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Astrocyte-mediated immune modulation during mycobacterial infection
… are target cells for non-virulent and virulent mycobacterial strains. Extensive transcriptomic analysis of astrocytes revealed elevated expression of multiple pathways in infected cells, particularly those involved in inflammation and immune regulation, and emphasised the innate immune …
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Synthesis of Mycobacterial Ergothioneine biosynthetic pathway metabolites
Many gram positive bacteria, such as Mycobacterium tuberculosis, lack the redox protective molecule, glutathione and produce mycothiol (MSH) and ergothioneine (ESH) as their principal low molecular mass thiol instead. Ergothioneine has been known for a while as an anti-oxidant; however its role as …
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Strategies to improve treatment of nontuberculous mycobacterial disease
Contains fulltext : 162523.pdf (Publisher’s version ) (Open Access)
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Detection of mycobacterial siderophores and implications for diagnostics
… simple assay for detection and quantification of mycobacterial siderophores that has potential applications in tuberculosis (TB) diagnosis. This assay has been tested and was successfully able to detect mycobactin spiked into a biological specimen. Furthermore, the theoretical level of mycobactin …
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Functional Characterisation of the Mycobacterial Virulence Protein Erp
… must continually resist host defences. A major mycobacterial survival mechanism lies in the unusual and complex cell envelope. The mycobacterial cell envelope provides a robust permeability barrier that helps the bacteria tolerate the variety of host insults it encounters. Many mycobacterial …
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Experimental Models of Mycobacterial Replication and Killing in Macrophages
… a granuloma may contribute to the control of mycobacterial infection. The experiments described here demonstrated that both apoptosis [Fas-mediated] and necrosis [perforin-induced] of infected human monocytes do not affect the survival of intracellular mycobacteria in an in vitro infection …
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Jak-stat pathway in the control of mycobacterial infections
… the in vivo functions of the JAK/STAT pathway in mycobacterial infection. Flies contain only one JAK (hop), one STAT (STAT92E), and three identified interleukin-like signals to activate signalling (upd, upd2, upd3).<br/>I show that, in Drosophila, the STAT-activating cytokine Upd3 is harmful to …
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Cell Wall Lipids Promoting Host Angiogenesis During Mycobacterial Infection
<p>Mycobacterial infection leads to the formation of characteristic immune cell aggregates called granulomas. In humans and animal models, tuberculous granuloma formation is accompanied by dramatic remodeling of host vasculature which ultimately benefits the infecting mycobacteria, suggesting the …
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Understanding functional mechanisms of genetic susceptibility to mycobacterial infection
… cells that play critical roles during mycobacterial infection. I demonstrated that in these cells ASAP1 is essential for migration and phagocytosis of mycobacteria. I characterized proteins that ASAP1 interacts with during mycobacterial infection. Finally, I found that the …
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Barriers to tuberculosis drug discovery: the mycobacterial cell wall
The mycobacterial cell wall is a highly complex macromolecular structure that provides intrinsic resistance to several anti-tuberculosis drugs, making it critical in the success of Mycobacterium tuberculosis infection. Multiple layers encapsulate the cell membrane, including arabinogalactan chains …
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Involvement of endothelial cells and macrophages in mycobacterial infections
… establishment, and subsequent dissemination of mycobacterial infection. The present study was undertaken to investigate the phagocytosis of mycobacteria by endothelial cells and macrophages and the subsequent activation of these host cells after infection with the bacilli. Endothelial cells, …
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Mycobacterial strain diversity : impact on the host immune response
Bibliography: p. 169-207.
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Zebrafish Mutagenesis Study of Host Determinants of Mycobacterial Infection Outcome
… screen in zebrafish for host determinants of mycobacterial infection outcome that I participated in. Zebrafish larvae from families randomly mutagenized with N-ethyl-N-nitrosourea were infected with Mycobacterium marinum and monitored over time. Larvae with increased susceptibility to …
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Chemical Probes and Strategies to Study Mycobacterial Cell Envelope Assembly
… chemical approaches to study and manipulate mycobacterial cell envelope biosynthesis. Specifically, we present biosynthetic glycan labeling strategies that leverage the activity of native glycosyltransferases to probe arabinogalactan and mannose-containing glycolipids. Building upon prior …
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