Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 124 for “"Mutational analysis"”.
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Mutational analysis of the West Nile Virus NS4B protein
… project used protein modeling and phylogenetic analysis of the NY99 WNV NS4B protein in comparison to NS4B proteins from other flavivirus and WNV strains to identify amino acid residues with a theoretical probability of contributing to the function of NS4B. The second aim utilized site-directed …
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Mutational analysis of the tobacco etch potyvirus cylindrical inclusion protein
The CI protein of tobacco etch potyvirus is known to be an NTPase as well as a putative RNA helicase. In a previous study, the CI gene and three mutants were constructed and expressed in the E. coli expression vector pET 11a. In this study the TEV CI gene was cloned into E. coli expression vector …
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A Mutational Analysis of the Period Locus of Drosophila Melanogaster
… product can mimic these results. DNA sequence analysis has revealed that in per<sup>0</sup> flies, a single nucleotide change resulted in a translational stop codon and hence a truncated protein. A valine-to-aspartic acid change in the per<sup>1</sup> mutants lengthens period. Likewise the …
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Mutational Analysis of CD127 and Its Role in Immunological Diseases
Interleukin (IL) -7 is an essential non-redundant cytokine that influences T-cell differentiation, proliferation, homeostasis and T-cell functions. In T-cells, IL-7 signals are transduced via IL-7's heterodimeric receptor composed of a common, γ chain (CD132) and an IL-7 specific, α chain (CD127). …
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Mutational analysis of JIL-1, a tandem kinase in Drosophila
… were identified and 4 were selected for further analysis. The mutant phenotypes given by the four new JIL-1 alleles were characterized. Three of the four showed polytene chromosome phenotypes similar to those of individuals homozygous for a null allele of JIL-1, and one showed polytene chromosome …
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Mutational Analysis of NBU1 Attachment Sites Reveals a Novel Recombination Mechanism
… been used to determine the minimal attN1 site. Mutational analysis of the attN1 site has indicated that several nucleotide sequence motifs in the attN1 site are required for integration. Analysis of attN1 and attBT1-1 mutations has also led to the conclusion that recombination mechanism of NBU1 …
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Mutational Analysis of HIV-1 Nucleocapsid Protein and Methods Development for Aptamer Discovery
… projects. The first part of the thesis is on the mutational and combinatorial analysis of HIV-1 nucleocapsid protein (NCp7). Sixteen mutants of NCp7 were constructed and over-expressed in <em> E. coli </em> . The dissociation constants (K <sub>d </sub> ) between the mutant proteins and the major …
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Mutational analysis of integrase and excisionase proteins in bacteriophage lambda site-specific recombination system
Second-site reversion analyses were performed on 21 recombination defective Int mutants. A second-site revertant P243L/E218K and a pseudorevertant E175K were isolated and further characterized using genetic and biochemical methods. The results showed that the second-site substitution E218K …
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Localization and Mutational Analysis of the Nuclear and Aggregation-Prone Ime4 Protein in Saccharomyces cerevisiae
… the protein in a controlled manner. Lastly, mutational analysis was conducted to determine which regions of the protein were necessary for its nuclear localization, aggregation, and sporulation function.</p>
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Mutational analysis of Hsp110 suggests an integral role for the chaperone in yeast prion propagation.
… G616D may disrupt PKA signaling. Comparative analysis of Sse1 and Sse2 reveals that Sse1 is more thermostable than Sse2 and Sse1 propagates [PSI+] efficiently whereas Sse2 fails to promote [PSI+] propagation. Small structural differences between the proteins may be responsible for this. …
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HIV entry : a biophysical and mutational analysis of gp41-mediated membrane fusion and its inhibition
The experiments described in this thesis were designed to elucidate the manner in which the HIV-1 envelope protein (Env) initiates infection of host cells, and to develop inhibitors of viral entry. Env comprises two non-covalently attached subunits, gpl20 and gp41, that associate as a trimer on the …
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Mutational analysis of the human histone chaperone, Nap1, in nucleosome disassembly at the HTLV-1 promoter
The human genome is packaged to fit within the confines of the nucleus through the interaction with four core histone proteins, H2A, H2B, H3 and H4. These proteins organize the genetic material, however they also make it difficult for the cells to access the information stored within the DNA …
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Mutational analysis of the Vam3 transmembrane domain and its effects on vacuole fusion in Saccharomyces cerevisiae
Vam3 is a SNARE protein essential in yeast vacuole homotypic fusion. The specific role of the Vam3 trans-membrane domain (TMD) during vacuole fusion remains unclear. Previous work had shown that replacing Vam3 TMD with a prenyl lipid anchor (CCIIM) results in attenuated fusion and accumulation of …
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Mutational analysis of the DNA mismatch repair genes, hMLH1 and hMSH2, in South African colorectal cancer patients
… single strand conformation polymorphism (SSCP) analysis. Sixteen pathogenic mutations were found in 14 tumours, 10/22 (45%) from black patients and 4/10 (40%) from caucasian patients. Five tumours presented with two mutations each, one is a compound heterozygote and the other four tumours are …
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Mutational Analysis to Define the Functional Role of the Third Intracellular Loop of D1-Class Dopaminergic Receptors
The third intracellular loop (IL3) and cytoplasmic tail (CT), which are the most divergent regions between human D1-class dopaminergic receptors (hD1R and hD5R), have been implicated in modulating their subtype-specific functional phenotypes. The importance of the IL3 for Guanine nucleotide-binding …
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A Mutational Analysis of Bacteriophage Lambda Site-Specific Recombination (In Vitro, Dna-Dna Interaction, Protein, Oligonucleotide-Directed)
… the five Int arm-type binding domains. Footprint analysis and recombination analysis of individual and collective mutations demonstrated that Int interacts with unique arm-type sites during integrative and excisive recombination.
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Mutational Analysis of the Export Targeting Motif of Fibroblast Growth Factor 2, a Mediator of Tumor-Induced Angiogenesis
… In the second part of this thesis, a systematic mutational analysis of the FGF2 open reading frame was conducted in order to identify cis elements that direct FGF2 to its export machinery. Initial experiments revealed the identification of FGF2 mutants that are defective in binding to heparan …
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A mutational analysis of the roles of cytoplasmic domains of the gonadotropin-releasing hormone receptor in coupling and internalization
The G protein-coupled receptor (GPCR) family is the largest group of homologous proteins in the human genome. GPCRs are of prime physiological and medical importance as the actions of a wide range of hormones and drugs are mediated by these receptors. The gonadotropin-releasing hormone (GnRH) …
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A Mutational Analysis of XcpQ, the Outer Membrane Component of the Type II Protein Secretion System From Pseudomonas Aeruginosa
… are thought to pass. In this thesis I present a mutational analysis of the role of XcpQ in Type II protein secretion. A series of XcpQ mutants with single amino acid substitutions were identified by a multilevel screening process designed to isolate mutants with minimal function but near wild …
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