Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Mutant population"”.
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Development of a Transposon Based Activation Tagged Mutant Population in Tomato for Functional Genomic Analysis
… is a need to study functional genetics through mutant lines that exceed the practical limitations imposed by the popular research cultivar, Micro-Tom. This project utilized Agrobacterium transformation and the transposon tagging construct, Ac-DsATag-Bar_gosGFP, to produce activation tagged and …
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Seed germination, kanamycin sulfate selection, and the influence of nitrogen treatments on an insertional mutant population of Fragaria vesca
… methods of generating unique Ac/Ds insertional mutants in a population of Fragaria vesca, various methods of seed germination, kanamycin screening, and the effects of varying nitrogen fertilization on diploid strawberry have been examined. Seed germination was improved to 42% in B5 liquid medium …
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The Response of Tepary Bean (Phaseolus actifolius) Germplasm to Induced Mutation
See the attached abstract below
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Improved regeneration and Agrobacterium-mediated transformation of wild strawberry (Fragaria vesca L.)
… protocol to generate an insertional mutant population to support the justification of F. vesca as a model organism for rosaceous crops. The transformation techniques described by Alsheikh et al. (2002) and Oosumi et al. (2005) were modified and applied to a range of germplasm obtained …
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The role of space in homeostasis and preneoplasia in stratified squamous epithelia
… tissue. The studied dynamics of Notch and p53 mutant clones in mouse epithelia has been found to be highly distinct, with the former fully colonizing the tissue whereas the latter only partially. I demonstrated that the two mutants’ tissue takeover dynamics can be recapitulated by two distinct …
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Double-Strand Break Repair Pathways In Dna Structure-Induced Genetic Instability
… pathway(s) available; however, the type of mutants produced after repair was greatly dictated on the available DSB repair system, indicated by the shift from 2% large-scale deletions in the total mutant population to 24% large-scale deletions when NHEJ was present (4). This suggests that …
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Characterization of T-DNA integration sites within a population of insertional mutants of the diploid strawberry Fragaria vesca L.
… collection of several hundred insertional T-DNA mutants at Virginia Tech, using either of two commercially available vectors, pCAMBIA 1302 and 1304. In this study, we have used expression of the green fluorescent protein (GFP) as a tool to identify homozygous mutant lines. Three different …
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Elucidating the role of jasmonate metabolites in jasmonic acid signaling
… metabolic pathways of JA and respective mutants blocked in such pathways are providing a unique opportunity to study the homeostatic regulation of JA and its biological function in plant stress responses. Two chapters of this thesis presents the physiological and signaling function of …