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Showing 1 to 20 of 58 for “"Mutant Proteins"”.
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Biochemical characterizations of Escherichia coli DnaK and DnaK mutant proteins purified from ndk deficient cells
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 1999.
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Relating the structure of the HSV-1 UL25 DNA packaging protein to its function
… in a protein expression plasmid. A series of mutant proteins was generated, each protein containing a deletion of the unstructured residues in one of the six regions, L1-L6. Another set of mutant proteins were constructed with each member containing substitutions of selected amino acids within …
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A genetic and biochemical analysis of LexA repressor cleavage in Escherichia coli K-12.
… to stimulate autodigestion. A set of lexA (Ind⁻) mutants that are deficient in in vivo RecA-mediated cleavage but retain significant repressor function were isolated. These 20 mutations resulted in amino acid substitutions in 12 positions, most of which are conserved between LexA and four other …
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Studies on the regulation in vivo and structure/function relationships of the enzyme 5-phosphoribosyl-alpha-1-pyrophosphate synthetase from Salmonella typhimurium
… directed mutagenesis and characterization of mutant proteins were used to examine possible residues involved in catalysis. Initial studies of the transcriptional regulation of the prs gene were also conducted.
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Aspects of the hemes and modulation of hydrogen donors in catalases from bovine liver, yeast, and escherichia coli
… H202. Expression of the genes encoding these proteins is controlled by different regimes. HPJI is thought to be a hexamer, having one heme d cis group per enzymatic subunit. HPII wild type protein and heme containing mutant proteins were obtained from the laboratory of P. Loewen (Univ. of …
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Characterization of a novel interaction between presenilin-1 and monoamine oxidase-A
… over-expression of different AD-related PS-1 mutant proteins, i.e. Y115H, ΔEx9 and M146V, in hippocampal-derived HT-22 cells alter either basal and/or Ca²⁺-sensitive MAO-A activity. The effects of PS-1 mutant proteins on the availability of intracellular Ca²⁺ are not consistent suggesting that …
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The prlC Suppressor Phenotype and Its Relation to Oligopeptidase a Specificity
… to the active site of OpdA. Because the PrlC mutant proteins suppress many different signal peptide mutations with no changes in the active site of OpdA, it is likely that wildtype OpdA also recognizes substrates of different lengths and sequences.
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Characterization of the Anti-Inflammatory Effects of the Molluscum Contagiosum Virus MC160 Protein
Using a series of MC160 deletion mutant proteins, we found that two distinct regions of the MC160 protein independently inhibit TNF-mediated NF-kappaB activation. Specifically, we observed that the second DED of MC160 interacts with procaspase-8 as a means to inhibit NF-kappaB activation, while the …
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The characterization of human [gamma]D-crystallin mutants and their differential interactions with the lens chaperone [alpha]B-crystallin
… aggregation or precipitation of crystallin proteins results in the formation of large structures that scatter light, preventing the pinpoint focusing on the retina normally accomplished by the lens. The human eye lens is composed of fiber cells packed with crystallins up to 450 mg/ml. Human …
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COMPOSTI AD AZIONE ANTI-AGGREGANTE ESERCITANO UN EFFETTO BENEFICO SU MODELLI CELLULARI DI ATROFIA MUSCOLARE SPINALE BULBARE
… AR function and the aggregation process of the mutant SBMA protein. In this study we tested in a PC12 cell model of SBMA (112 polyGln repeats AR expressed through an inducible system) six compounds, AR antagonist or partial agonists analogs, defined as SARMs (Selective AR Modulator), known to …
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Essential Fatty Acid Biosynthetic Enzymes of Escherichia Coli and Lactococcus Lactis Subsp. Lactis
… products. I report the first characterized fabG mutants. By chemical mutagenesis followed by a tritium suicide procedure, I obtained three conditionally-lethal temperature-sensitive fabG (fabGts) mutants. The E. coli mutant has two point mutations: A154T and E233K. In Salmonella enterica Serovar …
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Single-Stranded-Dna Sp6 and T7 Bacteriophage Promoter Plasmids for Engineering Mutant Rnas and Proteins: Extensions and Deletions at the Amino Terminus Which Alter the Activity of Preproparathyroid Hormone Signal Peptide
… enzymatic sequence analysis, and expression of mutant proteins, all from a single vector. Chimeric phage-plasmid expression vectors, or phagemids, containing the intergenic region of the single stranded DNA bacteriophage f1 and either a bacteriophage SP6 or T7 promoter have been constructed. …
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Contributions of aromatic pairs of human Gamma-D-Crystallin to its folding, stability, aggregation, and interaction with human Alpha B-Crystallin
Two distinct groups of proteins, a-crystallins and [Beta][gamma]-crystallins, constitute 90% of the vertebrate eye lens soluble proteins. Long-term solubility and stability against unfolding and aggregation are essential properties of crystallins and crucial to the function of the lens. Aggregation …
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X-ray diffraction analysis of gene V protein encoded by filamentous bacteriophage Ff
… wild-type and isoleucine-47 $\to$ methionine mutant proteins with x-ray diffraction data phased to 2.5 A resolution. The structure of the wild-type protein was refined to an R factor of 19.1% using native data to 1.8 A resolution. The gene V protein monomer is largely composed of …
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Molecular and Kinetic Characteristics of wild type and mutant Porphobilinogen deaminase
… and mechanism. Two clinically relevant PBGD mutants, the K98E and K98R were expressed. Both of these mutants have previously been described in patients. We engineered and expressed an additional mutant, K98A, in order to investigate the effect of charge at this residue. The K98E, K98R and …
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Molecular and Kinetic Characterisation of wild type and mutant Porphobilinogen Deaminase
… and mechanism. Two clinically relevant PBGD mutants, the K98E and K98R were expressed. Both of these mutants have previously been described in patients. We engineered and expressed an additional mutant, K98A, in order to investigate the effect of charge at this residue. The K98E, K98R and …
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Mutational Analysis of HIV-1 Nucleocapsid Protein and Methods Development for Aptamer Discovery
… studies related to the interaction between proteins and nucleic acids and their applications. The work reported here includes two projects. The first part of the thesis is on the mutational and combinatorial analysis of HIV-1 nucleocapsid protein (NCp7). Sixteen mutants of NCp7 were …
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The self-assembly of proteins: Probing anisotropic protein-protein interactions using phase diagrams
… However for biological macromolecules such as proteins, anisotropic interaction energies and multiple degrees of freedom dominate the behaviour, making predictability of solution behaviour and crystallization extremely complex. To probe the impact of anisotropic interactions on the …
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The expression and role of Migration Stimulating Factor (MSF) in oral tumours
… studies, the effects of rhMSF (wild type and mutant proteins) were examined on human cell lines TYS, HSG, Endo 742 and FSF44. These cells were derived from OSCC, SGT, microvascular endothelial cells and skin fibroblasts, respectively. For ex-vivo studies, paraffin embedded archival specimens …
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Design and development of an electrochemical and infrared spectroscopic medical device for serum-based cancer diagnostics
… classification, highlighting differences in proteinsecondary structures within clinical serum samples. The integrated diagnostic platformfurther demonstrated the ability to electrochemically detect IDH1-R132H proteins inspiked buffer samples at 0.05-10,000ng/ml concentrations with R2 = 0.958. …
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