Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Mutant Mouse Model"”.
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The Role of CLOCK in Regulation of Dopamine Neurotransmission in the CLOCKdelta19 Mutant Mouse Model
… firing and bursting was elevated in Clockdelta19 mutants with the most significant deviations early in the light cycle. Mimicking this increase in dopaminergic activity using optogenetic targeting resulted in decreased anxiety-related behavior similar to the Clockdelta19 phenotype. Consistent with …
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Lung Defects Contribute to Respiratory Symptoms in a Mecp2-mutant Mouse Model of Rett Syndrome
… the Mecp2-deficient lung, we utilized a Mecp2-mutant mouse model that recapitulates many features of RTT. I found striking lipid metabolism abnormalities in the lungs of Mecp2-mutant mice, including increased cholesterol and triglycerides and decreased phosphatidylcholines. My single cell …
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Vitamin D Modulates Rett Syndrome Phenotypes and Underlying Cellular Pathways in an Mecp2-mutant Mouse Model
… ameliorate the many Mecp2-null phenotypes in RTT mouse models. Using an in vitro approach, I determined that adding the activated form of vitamin D to Mecp2-knockdown cortical neurons reduces the aberrant NF-kB activation and promotes neurite outgrowth. Further, VitD dietary supplementation …
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The Fanconi anemia pathway and HELQ work alongside dormant replication origins to suppress replication-associated genome instability
… the <italic>Mcm4<super>chaos3</super></italic> mouse model, we found that dormant replication origins, which act as backup initiation sites, play a critical role in the recovery of stalled replication forks. A reduced number of dormant origins in these mice led to persistently stalled forks, …
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The Role of E2F1 In The Response to Dna Double Strand Breaks
… is phosphorylated at serine 31 (serine 29 in mouse) by the ATM kinase as part of the DNA damage response. To investigate the importance of this event, our lab developed an E2F1 serine 29 mutant mouse model. I find that E2F1 serine 29 mutant cells show loss of E2F1 foci formation in response to …
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Beyond the rare blood group Vel, uncovering the functions of SMIM1 in blood and in other organs
… main approaches (in vivo and in vitro) and three models (human volunteers, mouse and cell lines). First, using available datasets, I showed that SMIM1 is expressed in megakaryocytes (MKs), platelets, neutrophils and naïve, memory and class-switched B-lymphocytes. I further demonstrated that SMIM1 …
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Deciphering ARAP3 Functions in Hematopoiesis and Hematopoietic Stem Cells
… several conditional knockout (CKO) transgenic mouse models in addition to the KI/KI mutant mouse model. Here, we report that HSCs from surviving adult KI/KI bone marrow (BM) are compromised in their ability to self-renew and reconstitute recipient mice. To decipher the possible mechanisms of …
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Notch activation and clone fate in intestinal homeostasis and inflammation
… methods with a transgenic Notch active mutant mouse model (N1ICD<sup>ΔIEC</sup>), cell and clonal dynamics were determined in the normal and inflamed colon. Nucleotide analogue labelling data found N1ICD<sup>ΔIEC</sup> crypts had an increased number of proliferative cells with shorter …
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Developmental Deregulation and Tumorigenesis Inhibition In 14-3-3Zeta Knockout Mouse
… I generated a strong 14-3-3z hypomorphic mutant mouse model by Gene Trap. We found that the homozygous mutant mice are lethal neonatally due to respiratory failure. This lethality could be rescued when outbred to CD-1 or backcrossed to FVB/N mouse strain.</p> <p>Early mammary gland …
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Differential Expression Of Proteins Involved In VLDL Trafficking Causes Reduced VLDL Secretion In Male Ames Dwarf Mice
… secretion in Ames dwarf (Prop1df, df/df) mice, a mutant mouse model homozygous for a recessive mutation at Prop1 gene locus (Prop1df) having deficiency of growth hormone (GH), thyroid stimulating hormone (TSH) and prolactin (PRL). This model is characteristic of prolonged longevity (~50% longer) …
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Gene therapy provides long-term visual function in a pre-clinical model of retinitis pigmentosa
… However, despite the need for treatment, mouse models with mutations in the alpha subunit of PDE6 have not been characterized beyond 1 month of age or used to test the pre-clinical efficacy of potential therapies for human patients with RP caused by mutations in PDE6A. We first proposed to …
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NEW PHARMACOLOGICAL TOOLS FOR AUTISM RESEARCH: OXYTOCIN RECEPTOR MUTANT MICE AND ZEBRAFISH AS NEUROBEHAVIOURAL MODELS
… perspective, to characterize specific animal models in order to validate the use of OT analogs with more suitable characteristics for preclinical research. To this end, a characterization of the behavioural phenotype of OTR knock-out mice (OTR-/-) and heterozygous littermates (OTR+/-) in …
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Calcium Channel Dysfunction in Huntington’s Disease
… were conducted using the transgenic R6/2 HD mouse model, which, has a CAG expansion of approximately 144 repeats and displays progressive motor and behavioral dysfunction similar to those found in HD patients. The patch clamp method was used to record whole-cell HVA Ca<sup>2+</sup> currents …
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Anti-Cancer Effects Of Garcinol In Pancreatic Cancer Transgenic Mouse Model
… effects of dietary Garcinol in a transgenic mouse model of Pancreatic Cancer (PaCa). This model is considered to be the animal model which best mimics development of human PaCa. Based on <em>invitro</em> data from our lab we hypothesized that Dietary Garcinol treatment will slow down the …