Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Mutagenesis Study"”.
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Zebrafish Mutagenesis Study of Host Determinants of Mycobacterial Infection Outcome
This thesis describes a large-scale forward genetic screen in zebrafish for host determinants of mycobacterial infection outcome that I participated in. Zebrafish larvae from families randomly mutagenized with N-ethyl-N-nitrosourea were infected with Mycobacterium marinum and monitored over time. …
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The anti-fluorescein antibody active site: A single-chain site-specific mutagenesis study
Restriction data tranferred 2014-07-01T11:21:50-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission
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Deciphering the mechanism of Transferrin Receptor mRNA degradation under iron replete conditions
… precise mechanism of which is yet unknown. This study was based on three hypotheses that could lead to identification of the mechanism of TFRC-1 mRNA degradation. First, there could be a microRNA mediated silencing of the TFRC-1 mRNA. Secondly, the TFRC-1 mRNA could catalyse its own degradation …
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Recombinant production and regioselectivity study of lytic polysaccharide monooxygenases
… amongst LPMOs. In this thesis, an extensive mutagenesis study was performed to better understand the determinants of this oxidative regioselectivity. For this, four fungal and two bacterial LPMO representatives were produced in Pichia pastoris and Escherichia coli, respectively. Carefully …
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Biosynthesis of the antimicrobial peptide epilancin 15X
… based on its x ray crystal structure and a mutagenesis study. The characterization of ElxP demonstrated that the protease can cleave the unmodified precursor peptide and relies on the C-terminal residues of the leader region for substrate recognition. Finally, the stereochemical …
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Discovery of function in the enolase superfamily
… the capping domain loops were investigated via a mutagenesis study of GlucD.
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Studies of Cytochrome C Oxidases From Rhodobacter Sphaeroides and Vibrio Cholerae
… to be the sequence of CXXCH. Site-directed mutagenesis studies of the various combined mutants of the motif show that the cysteine and histidine residues are critical for the incorporation of heme c into cbb3 enzymes from Rhodobacter sphaeroides and Vibrio cholerae. Results also show that …
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Studies of cytochrome c oxidases from Rhodobacter sphaeroides and Vibrio cholerae
… to be the sequence of CXXCH. Site-directed mutagenesis studies of the various combined mutants of the motif show that the cysteine and histidine residues are critical for the incorporation of heme c into cbb3 enzymes from Rhodobacter sphaeroides and Vibrio cholerae. Results also show that …
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Pervasive degeneracy and epistasis in a protein-protein interface
… I performed structure-guided and library-based mutagenesis studies of bacterial two-component signaling pathways. These pathways, typically consisting of a histidine kinase and a response regulator, are an ideal model system for studying protein-protein interactions as they rely almost …
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Mechanistic studies of lanthipeptide biosynthesis
… available for this class of proteins before the study presented in chapter 5 with respect to the cytolysin synthetase CylM. Unexpectedly, the CylM dehydratase domain resembles the catalytic core of lipid kinases despite the absence of notable sequence homology. Mutagenesis study of CylM provides …
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Anaerobic, NADH-Dependent Haem Breakdown in a Family of Haemoproteins
… enterocolitica}$ protein, HemS. To study the features of this reaction in more detail, conventional experimental methods were combined with Energy Landscape Theory. Deuterium labelling demonstrated that the reaction was initiated by hydride transfer and stopped-flow spectroscopy …
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Regulation of BAX/BAK-Dependent Cell Death Program
… of anti-apoptotic BCL-2 members. Here, a BAX/BAK mutagenesis study indicated that anti-apoptotic BCL-2 members prevent BAX/BAK activation by sequestering activator BH3s rather than by directly binding to BAX/BAK. I further demonstrated that inactivator BH3s are able to displace activator BH3s from …
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Enzymology And Medicinal Chemistry Of N5-Carboxyaminoimidazole Ribonucleotide Synthetase : A Novel Antibacterial Target
… On the basis of these models, a site-directed mutagenesis study was subsequently conducted that focused on five amino acid residues located in the active site region of the enzyme. These investigations demonstrated that Asp153 and Lys353 play critical roles in catalysis without affecting …
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Mechanistic investigations on polyprenyl transferases and multi-target antibiotic discovery
… phosphatase (UPPP)) deducted from transposon mutagenesis. Putative inner membrane family proteins YubA and YubD are annotated as transporters and may also be targets because the compounds collapsed the proton motive force in membrane vesicles. Cis-prenyl transferase UPPS serves as a lipid …
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Exploring the molecular signatures of the ligand-receptor-Gi/o protein interactions of adenosine A1 and A3 receptors
… of 39 at hA3R was explored through extensive mutagenesis studies and the key molecular signatures accounting for the improved affinity of 39 were decoded. These new lead compounds represented excellent hAR antagonist candidates for investigating the structural selectivity filers in the …
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Pharmacological and Pharmacokinetic Studies of a K<sub>Ca</sub>2.2 Positive Allosteric Modulator
<p>Small-conductance Ca<sup>2+</sup>-activated potassium channels (K<sub>Ca</sub>2.x) family is widely expressed in neurons, the heart, and endothelial cells. K<sub>Ca</sub>2.x channels are named small conductance Ca<sup>2+</sup>-activated potassium channels due to their comparatively low …
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Pushing the limits of aldoxime dehydratases for the synthesis of industrial relevant nitriles and their use in chemo- and biocatalytic cascade reactions
… towards aromatic aldoximes by a semi-rational mutagenesis study. This was achieved by a systematic activity study of OxdRE and mutants for a library of 22 different substituted aldoximes, which revealed that OxdRE-Wt has high activity for ortho-substituted aromatic aldoximes and that the mutant …
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Library Screening and Focused Multiomics of Antibacterial Action on Vancomycin-Resistant Enterococci faecium
… and high (4x MIC) of an antibiotic. Furthermore, mutagenesis study was also done using MRSA. In this study, mutants of MRSA were generated on resistant antibiotic plates. It was followed by the extraction of genomic DNA which was later used to study whole genome sequence of MRSA. This study helped …