Global ETD Search
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Showing 1 to 20 of 24 for “"Munc18"”.
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Reconstitution of Munc18-Dependent Mast Cell Degranulation
… called SNAREs are regulated by the Sec1/Munc18 (SM) protein family (Axle Lorentz, Baumann, Vitte, & Blank, 2012). Mast cells express three mammalian Munc18 isoforms (a, b, and c), which are linked to SNARE-dependent exocytosis in numerous organisms. However, the exact roles of each of …
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Munc18 function in large dense-core vesicle exocytosis
… essential in later steps of exocytosis. The Sec1/Munc18 proteins are also essential in exocytosis and other membrane trafficking processes but their molecular and cellular function is less clear than that of the SNARE proteins.In our work we studied the role of Munc18 in large dense-core vesicle …
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Structural and Functional Studies of Munc18 and SNARE Proteins
… of this machinery are proteins from the Sec1/Munc18 (SM) and SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptors) families. SNAREs function by forming a four-helix bundle called SNARE complex; assembly of the SNARE complex brings two membranes together and is key for …
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Munc18-1 Is a Master Orchestrator of Synaptic SNARE Assembly
Pages 114-185 are misnumbered as pages 115-186.
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Insights into the Functions of Munc18-1 in Neurotransmitter Release
… membrane fusion machinery in neurons includes Munc18-1 and the SNARE proteins syntaxin-1, SNAP-25 and synaptobrevin. The SNAREs form tight SNARE complexes that bring the vesicle membrane and plasma membrane into close proximity and provide forces to induce membrane fusion. Munc18-1 is essential …
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Unraveling the Molecular Mechanisms of Munc18 and Munc13 in Mast Cell Exocytosis
… is regulated by indispensable proteins such as Munc18 and Munc13 through physically interacting with SNARE proteins. Disruption of proteins that are involved in the secretory granule exocytosis causes serious immune disorders including familial hemophagocytic lymphohistiocytosis. However, …
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The Role of Munc18 Proteins in Physiologic and Pathologic Exocytoses in the Pancreatic Acinar Cell
… whose assembly is modulated by distinct Munc18 proteins. The Munc18 isoform responsible for these exocytotic events in the acinar cell is unknown. Here, I postulate Munc18b to regulate apical exocytosis in the acinar cell. Current dogma for the pathogenesis of acute pancreatitis, …
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Fluoreszenzmikroskopische Studien an Plasmamembranen zur Untersuchung der molekularen Mechanismen der neuronalen Exocytose
… Membranen fusioniert. Neben den SNAREs ist auch Munc18-1, das zur Sec1/Munc18-Proteinfamilie gehört, ein essentielles Protein der neuronalen Exocytose. Der Munc18-1-Knockout führt zum Block der neuronalen Exocytose. In vitro bildet Munc18-1 mit Syntaxin1 einen Komplex, der Syntaxin1 in einer …
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Fluoreszenzmikroskopische Studien an Plasmamembranen zur Untersuchung der molekularen Mechanismen der neuronalen Exocytose
… Membranen fusioniert. Neben den SNAREs ist auch Munc18-1, das zur Sec1/Munc18-Proteinfamilie gehört, ein essentielles Protein der neuronalen Exocytose. Der Munc18-1-Knockout führt zum Block der neuronalen Exocytose. In vitro bildet Munc18-1 mit Syntaxin1 einen Komplex, der Syntaxin1 in einer …
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Unraveling the Functions of Synaptotagmin and Munc13 in Neurotransmitter Release
… sensitive factor adaptor protein receptor) and Munc18-1 as core components. Munc13, synaptotagmin and complexin underlie the exquisitely tight regulation of synaptic exocytosis. The SNAREs form the SNARE complex that brings synaptic vesicles and plasma membrane together. This complex is …
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Molecular Mechanisms of Presynaptic Plasticity and Function in the Mammalian Brain
… kinase C (PKC) isoforms that phosphorylate Munc18-1 proteins. It is unknown how generally applicable this mechanism is throughout the brain and if other proteins might be able to modulate PTP. Combining genetic (PKCαβy triple knockout [TKO] and Munc18-1SA knock-in [Munc18 KI] mice, in which …
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Structural and Functional Studies of the Munc13 MUN Domain and the RIM C2B Domain
… fusion machinery includes the SNARE proteins and Munc18- 1. Besides these universal components, many other neuronal specific proteins are also involved in regulating Ca2+-triggered neurotransmitter release, such as the key priming factors RIMs and Munc13s. Munc13s are essential for vesicle …
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Unraveling the Role of SNARE Interactions in Neurotransmitter Release
… a 'closed' conformation, which interacts with munc18-1, an essential protein in the neurotransmitter release. It has been proposed that the binding of munc18-1 might change the closed conformation. To test this model, I solved the solution structure of the N-terminal domain within the closed …
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Single-molecule fluorescence resonance energy transfer study of SNARE-mediated membrane fusion
… proteins, such as synaptotagmin, complexin and Munc18. By a new lipid mixing method at the single-vesicle level, we are able to accurately detect different stages of SNARE-mediated membrane fusion including docking, hemi and full fusion via FRET value of single donor/acceptor vesicle pair. …
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Packaging and Secretion of Airway Mucins
… take place on a scaffold provided by a Munc18 protein. Prior work in the Dickey-Tuvim laboratory has shown that different Munc18 proteins mediate baseline and stimulated mucin secretion, indicating that different plasma membrane exocytic machines underlie baseline and stimulated mucin …
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Dissecting Roles for the Macromolecular Machinery Involved in Neurotransmitter Release
… in the presence of NSF-αSNAP requires both Munc18-1 and Munc13-1, and is facilitated by synaptotagmin-1. I proposed a model whereby Munc18-1 and Munc13-1 are critical for mediating vesicle priming as well as precluding de-priming by preventing trans-SNARE complex disassembly. Complexin-1 …
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An evolutionarily conserved regulatory mechanism for endosomal membrane trafficking
… complexes; a process that is regulated by Sec1p/Munc18 (SM) proteins. Membrane fusion has been conserved through evolution and hence a lot or our knowledge about the molecular mechanism that regulates membrane traffic has come from experimentally tractable model organisms such as Saccharomyces …
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Characterisation of the role of VPS33B in Vesicular trafficking in polarised Epithelial cells.
… fully characterised. Mutations in VPS33B, a Sec1-Munc18 protein, account for 75% of ARC patients. Reduced expression of VPS33B at both the RNA and protein level was demonstrated in all ARC syndrome patients, even if mutations were not identified in VPS33B. A novel protein POLARIN (PLRN) was …
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Regulation of membrane fusion by Tlg2p & Vps45p through the endosomal system of Saccharomyces cerevisiae
… precludes complex assembly. The Sec1p / Munc18 (SM) family are a conserved group of proteins that regulate membrane fusion through interactions with their cognate syntaxins. Formulation of unifying hypotheses describing how SM proteins function has been problematic, primarily due to the …
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Vesicle-associated Membrane Proteins in Airway Mucin Secretion
… between a SNARE complex and an SM (Sec1/Munc18) protein. One type of SNARE protein, a Vesicle-Associated Membrane Protein (VAMP), has been shown to regulate exocytosis in multiple cell types.</p> <p>Here, we report that VAMP8 has the highest gene expression among all VAMP isoforms in …
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