Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Munc13"”.
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Unraveling the Functions of Synaptotagmin and Munc13 in Neurotransmitter Release
… receptor) and Munc18-1 as core components. Munc13, synaptotagmin and complexin underlie the exquisitely tight regulation of synaptic exocytosis. The SNAREs form the SNARE complex that brings synaptic vesicles and plasma membrane together. This complex is disassembled by NSF/α-SNAP (soluble …
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Unraveling the Molecular Mechanisms of Munc18 and Munc13 in Mast Cell Exocytosis
… by indispensable proteins such as Munc18 and Munc13 through physically interacting with SNARE proteins. Disruption of proteins that are involved in the secretory granule exocytosis causes serious immune disorders including familial hemophagocytic lymphohistiocytosis. However, compared to …
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MUNC13 PROTEINS REGULATE STRAIN-DEPENDENT EFFECTS OF ETHANOL-MEDIATED INHIBITION OF SYNAPTIC VESICLE RECYCLING
Alcoholism is a chronically relapsing disorder characterized by an inability to remain abstinent from alcohol for prolonged periods of time. One of the major factors contributing to relapse is the development of profound anxiety during abstinence. Our lab has previously demonstrated that chronic …
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Mechanistic Insights into the Role of Munc13 in Synaptic Vesicle Docking, Priming, and Fusion
… vesicles to dock and prime them for release. Munc13 is one of the essential components of the docking, priming, and fusion machinery. To understand the role of Munc13 in docking and priming I attempted to structurally characterize the MUN domain and SNARE protein interactions using nuclear …
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Structural and Functional Studies of the Munc13 MUN Domain and the RIM C2B Domain
… such as the key priming factors RIMs and Munc13s. Munc13s are essential for vesicle priming. RIMs form a protein scaffold in the presynaptic nerve terminal. My studies have focused on the structures and functions of the Munc13 MUN domain and the RIM C2B domain. I have studied the structure …
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Insights into the Functions of Munc18-1 in Neurotransmitter Release
… to establish the vital functions of Munc18-1 and Munc13 in neurotransmitter release, I found that Munc18-1 displaces SNAP-25 from syntaxin-1/SNAP-25 complex to form Munc18-1/syntaxin-1 complex on liposomes in the presence of NSF/α-SNAP/ATP. When NSF/α-SNAP were incorporated in the lipid mixing …
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Dissecting Roles for the Macromolecular Machinery Involved in Neurotransmitter Release
… presence of NSF-αSNAP requires both Munc18-1 and Munc13-1, and is facilitated by synaptotagmin-1. I proposed a model whereby Munc18-1 and Munc13-1 are critical for mediating vesicle priming as well as precluding de-priming by preventing trans-SNARE complex disassembly. Complexin-1 also impaired …
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Funktionelle Analyse von Isoformen der CAPS-Proteinfamilie
… eine Domäne mit Homologie zu dem Primingfaktor Munc13-1 (MHD1). Es ist ein Ortholog von Unc-31, einem Genprodukt aus Caenorhabditis elegans, das im Nematoden multiple Funktionen des Nervensystems beeinflusst. Ziel der vorliegenden Arbeit war es, die funktionelle Bedeutung von CAPS bei der …
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Identifying regulators of cytotoxic T cell function through molecular and genetic screening
… of CTL cytotoxicity, $\it{Rab27a}$, $\it{Munc13-4}$ and $\it{Prf1}$, were targeted using CRISPR. The resulting samples were used to establish a flow cytometry-based assay that simultaneously measures CTL degranulation and target cell death. This assay enabled me to screen for mediators of …
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Structure and Function of Proteins Involved in Regulated Secretion: Synaptotagmins and Complexin
… as well as other required proteins such as Munc13 and Munc18. Complexin is a small soluble protein that binds to the SNARE complex with high affinity and regulates the formation of the SNARE complex. Synaptotagmin is the calcium sensor for fast release of neurotransmitter. Here I present …