Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Multiprotein Complexes"”.
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Gene expression in plastids of higher plants: evolutionary and functional aspects of different RNA polymerases - coordinated assembly of multiprotein-complexes
Plastid gene organisation maintains characteristics typical of its prokaryotic ancestry. The regulation of plastid gene expression however strongly deviates from that one its free-living cyanobacteria relatives. This intriguing complication of plastid gene expression characteristics is the result …
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Structural and Biochemical Investigation of the Pan2-Pan3 Deadenylase Complex
… is mainly carried out by two conserved multiprotein complexes: Pan2-Pan3 and Ccr4-Not. This thesis focuses on the in vitro characterisation of Pan2-Pan3. While Pan2-Pan3 and Ccr4-Not show poly(A) preference, it was unknown how the conserved exonucleases Pan2 and Caf1 recognise poly(A). …
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Anc1 : a new player in the cellular response to DNA damage
… is the only common member of seven different multiprotein complexes that all function in general RNA polymerase II-mediated transcription. Second, ANC1 is evolutionarily well-conserved between yeast and humans, suggesting that its function is critically important for survival. And finally, …
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Mechanisms of molecular switching in the Wnt signal transduction pathway
… depends primarily on the activities of three multiprotein complexes; the ‘degradasome’, which targets the central effector β-catenin for degradation in the absence of Wnt; the ‘signalosome’, which is assembled by Dishevelled upon Wnt-receptor binding to inactivate the degradasome, thus …
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Analysis of Human Protein Complexes by Quantitative Mass Spectrometry
… functions. Proteins predominantly act as part of multiprotein complexes to carry out these functions. The association of protein isoforms and post-translationally modified forms in protein complexes can influence their subcellular location, activity and substrate specificity. It is therefore …
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Expression of inflammasome-related molecules from U87MG cells cocultured with Naegleria fowleri in the non-contact system
… in humam. Inflammasomes are large intracellular multiprotein complexes that play a central role in innate immunity. They detect and respond to a large range of pathogen-associated molecular patterns (PAMPs), including bacterial flagellin, and damage-associated molecular patterns (DAMPs), such as …
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Photo-cross-linking and identification of nuclear proteins that bind to DNA containing a site-specific adduct of cis-[Pt(NH₃)(N-6-aminohexyl)-4-benzophenonamide)CI₂]
… survey the entire nuclear milieu, allowing for multiprotein complexes to stay intact and proteins to compete for binding to the damaged DNA has been difficult. These challenges were addressed by using a photo-active cisplatin analogue, cis-[Pt(NH3)(N-(6-aminohexyl)-4-benzophenonamide)C12] …
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The role of FOXK transcription factors in Wnt signal transduction
… and transduced through cytoplasmic and nuclear multiprotein complexes; the degradasome, signalosome and enhanceosome. The latter is tethered to enhancers by T-cell transcription factors (TCF), and core components include the chromatin reader Pygopus as well as the transcriptional repressor …
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Addition of flexible linkers to GPU-accelerated coarse-grained simulations of protein-protein docking
Multiprotein complexes are responsible for many vital cellular functions, and understanding their formation has many applications in medical research. Computer simulation has become a valuable tool in the study of biochemical processes, but simulation of large molecular structures such as proteins …
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Gcn5 Loss Impacts Myc-Driven Cancer In Mice and Human Cells
… the acetyltransferase module of SAGA and ATAC, multiprotein complexes involved in the modification of histone and nonhistone proteins. GCN5 is most recognized as a co-activator of gene transcription. The SAGA complex is recruited to chromatin by transcription factors such as MYC and E2F1 where …
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Recruitment of Enzyme Cascade to Phase-Separated Biomolecular Condensates Accelerates Reactions via Concentration-Dependent and Concentration-Independent Mechanisms
Biomolecular condensates are ubiquitous throughout biology, but their functions remain largely poorly understood. Biomolecular condensates concentrate biomolecules relative to the surrounding medium. For biomolecular condensates that concentrate enzymes and their substrates, classic enzyme kinetics …
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The regulation of yeast homotypic membrane fusion by class C ABC transporters
… two lipid bilayers into one continuous membrane. Multiprotein complexes that have been conserved in eukaryotes carry out the basic reactions of fusion. In Saccharomyces cerevisiae, homotypic vacuole fusion occurs in experimentally defined phases. Fusion priming does not involve contact between …
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Dissecting Roles for the Macromolecular Machinery Involved in Neurotransmitter Release
… one project, I investigated whether trans-SNARE complexes can be disassembled by NSF-αSNAP. I showed that trans-SNARE complex formation in the presence of NSF-αSNAP requires both Munc18-1 and Munc13-1, and is facilitated by synaptotagmin-1. I proposed a model whereby Munc18-1 and Munc13-1 are …