Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"Multiphoton Microscopy"”.
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Differential multiphoton microscopy
2010 Spring.
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Multifocal Multiphoton Microscopy for Neurobiological Imaging
… of therapeutics for their correction. Today, multiphoton excitation fluorescence microscopy is the method of choice for in vivo neuronal imaging with its inherent 3D resolution, minimal photo-damage, and excellent penetration depth. The study of neuronal interactions on dendritic arbor …
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Extending imaging depth of multiphoton microscopy
Two-photon excitation fluorescence microscopy has capability of deep tissue imaging with biological samples. However, because of the inhomogeneity of the refractive index in biological samples, the wavefront of the excitation light is often distorted. Due to the distortion of the wavefront, the …
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Multiphoton microscopy for stain-free slide-free histopathology
… labor-intensive. Label-free nonlinear optical microscopy, which produces high-resolution images with rich functional and structural information based on intrinsic molecular contrast, has demonstrated significant potential to overcome these problems by leveraging its nonperturbative nature and …
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Visualizing The Dynamics of Immune Surveillance In Brain Tumors By Intravital Multiphoton Microscopy
… surveillance in brain tumors by intravital multiphoton microscopy</strong></p> <p>By</p> <p>Felix I. Nwajei, MD</p> <p>Supervisory Professor: Tomasz Zal, Ph.D.</p> <p>Brain tumors (BTs) generally have a bad prognosis despite conventional treatment strategies. Immunotherapy is a relatively …
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Label-free multiphoton microscopy for imaging transient metabolic dynamics in living cells and tissue
… use of two-photon fluorescence lifetime imaging microscopy (2P-FLIM) of reduced nicotinamide adenine dinucleotide (NADH). This autofluorescent co-enzyme is involved in both aerobic and anaerobic metabolic processes which can be differentiated utilizing this advanced imaging approach. This thesis …
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Multimodal intravital imaging of tissue structure and cell dynamics in skin using integrated optical coherence and multiphoton microscopy
… microscope that integrates optical coherence and multiphoton microscopy. The development of the integrated microscope was focused on enabling sufficient performance of both modalities for in vivo imaging applications. This included the design of a dual-spectrum laser source that meets the …
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Cholinergic Regulation of Circadian Rhythms in the SCN: Characterization of Signaling Mechanisms and the Role of Intracellular Calcium in the Directionality of Clock Resetting
… in glutamate-like delay. Ca 2+ imaging using multiphoton microscopy revealed signal-specific differences in Ca2+ transients. Cholinergic-stimulated Ca2+ transients are localized to the microdomains in the cytoplasm, whereas glutamate-induced Ca2+ transients are predominantly uniform across the …
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Multiphoton Endoscopy
Multiphoton microscopy has the potential to be a versatile tool for clinical disease diagnosis and treatment, providing numerous benefits over current practices involving the histopathological analysis of biopsies. Specifically, multiphoton excitation has the unique ability to generate fluorescence …
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Functional Biological Spectroscopy and Multiphoton Imaging Using Endogenous Optical Contrast
… tissue, two model systems were investigated with multiphoton microscopy in order to evaluate processes important in angiogenesis and ovarian carcinogenesis research. Starting with an in vitro model of angiogenesis, the individual intracellular and extracellular optical signals were combined for …
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Tumor vasculature and microenvironment during progression and treatment : insights from optical microscopy
… By modifying the technique of intravital multiphoton microscopy (MPM) to perform phosphorescence quenching microscopy, I developed a non-invasive method to quantify oxygen tension (p02) in living tissue at high three-dimensional resolution. To probe functional changes in the metabolic …
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Mechanisms of Brain Region-Specific Amyloid-beta Deposition
… and aggregation. Parallel studies using in vivo multiphoton microscopy and in vivo microdialysis revealed that modest pharmacological reduction of soluble interstitial fluid: ISF) Aβ levels was associated with a dramatic reduction in amyloid plaque formation and growth. We found that ISF …
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Fibre Microstructure and Mechanics of Atherosclerotic Plaques
… were characterised through scanning electron microscopy, histology, and image processing. Local primary fibre orientation and the fibre dispersion were calculated. Plaque shoulder regions, where rupture is most frequent, had higher fibre dispersion and were more misaligned with the lumen wall. …
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Biomechanical and morphological characterization of common iliac vein remodeling: Effects of venous reflux and hypertension
… reflux and hypertension was quantified using multiphoton microscopy (MPM) and histological evaluation. Biomechanical results indicate that the veins stiffened and became less compliant when exposed to eight weeks of chronic venous reflux and hypertension. The mechanical stiffening was found to …
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Compact Femtosecond Lasers for Multiphoton Imaging
Multiphoton imaging currently is one of the main development directions in Laser Scanning Microscopy. A simplified way to assess suitability of a given laser for MPI applications is a figure of merit (FOM) approach based on peak power and average power of the particular excitation laser used. The …
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Development of a mucosal vaccine delivery system
… using LC/MS analysis and fluorescent multiphoton microscopy. The study suggests that lipid based nanoparticles possess adjuvant properties and can be potential candidate for enabling vaccine delivery via the nasal route.
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In Vivo Metabolic and Vascular Response to Hypoxia in Twist Knockdown Murine Breast Cancer
… determine vascular oxygenation within the tumor. Multiphoton microscopy was used to quantify metabolism on the cellular level by measuring the optical redox ratio as well as the relative contribution ratio of free and bound NADH. </p> <p>We found the optical redox ratio, vascular oxygenation, and …
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Modulation of Brain Tissue Transport and Endothelial Glycocalyx and Tight Junctions of the Blood-Brain Barrier by Transcranial Direct Current Stimulation
… coefficients (D<sub>eff</sub>). In vivo multiphoton microscopy was used to quantify D<sub>eff</sub> in rat brain 5-30 min post tDCS. A mathematical model was applied to further predict the effect of tDCS on the ECS width and ECM density.</p> <p>By combining a transport model for the BBB …
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Modeling Epithelial Ovarian Cancer In The Mouse
… p53/Rb-deficient OSE. Next, we have shown that multiphoton microscopy and second harmonic generation (MPM/SHG) imaging are well suited for studies of cell motility in vivo and can complement pathological characterization EOC by allowing optical biopsies of native human and mouse tissues at …
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Multimodal Optical Imaging for Tissue Characterization and Disease Diagnosis
… includes optical coherence tomography (OCT), multiphoton microscopy (MPM), and wide-field fluorescence imaging. The study is subdivided into four sections. In the first two sections, advanced algorithms and processing techniques are presented for rapid analysis and quantitative diagnostic …
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