Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Multiomics"”.
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Dissecting immune interactions in health and disease with multiomics and spatial technologies
… In the work of my thesis, I use high-throughput multiomics and spatial technologies to create comprehensive cell atlases. I focus on studying the immune cell communication among themselves and with other cells in the context of disease and development. Chapter 1 starts with an outline of the …
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Library Screening and Focused Multiomics of Antibacterial Action on Vancomycin-Resistant Enterococci faecium
Antimicrobial resistance is a major public health threat, and there is an urgent need for new strategies to address this issue. In a gram-positive bacteria, the peptidoglycan layer is thick as compared to gram negatives and is made up of peptide like polysaccharide chains. This peptidoglycan layer …
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Scalable Machine Learning Methods For The Analysis Of Single-Cell Transcriptomics And Multiomics Data
Transcriptomics and proteomics-based expression profiling technologies have become increasingly popular, more affordable, and more accurate in recent years. Expression profiling of expression at the single-cell resolution allows investigators to identify rare cell subtypes in human tissue which …
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Informatics Strategies for Alzheimer’s Disease Research Through Analyzing Genetics and Neuroimaging Data
… strategies within the Genomics, Molecular Multiomics, Biomarkers, and Outcomes (GMBO) framework to integrate large-scale biobank data and uncover key disease mechanisms. Through computational approaches, this work bridges genetic, molecular, and neuroimaging data to address critical …
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Development of a multi-omics approach to identify highly correlated transcriptomic, proteomic and metabolic signatures in maize B73 and FR697 drought stressed nodal roots
… that led to this remarkable ability, we analyzed multiomics (transcriptome, proteome, metabolome) datasets generated from the growth zone of nodal roots collected from the reference inbred line B73 and from inbred line FR697, which exhibits a relatively greater ability to maintain root elongation …
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Non-linear Multi Omics Data Integration Method Using Conditional Variational Autoencoders
… This is a novel contribution to the field of multiomics data integration, as no prior studies have explored the use of CVAEs for integrating multiple datasets in this context. The proposed architectures were tested on both real and simulated datasets. The results from both studies showed that …
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PROBABILISTIC AND DEEP LEARNING APPROACHES TO MODELING BIOLOGICAL SYSTEMS
… its performance on two different hematopoiesis multiomics datasets. Building on this, I present a computational analysis of lentivirally barcoded patient-derived organoids to show that epigenetic heritability plays a key role in drug resistance. This leads to a unified view where stable …
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Targeting PI3K Isoforms to Improve Effectiveness of T Cells Mediated Immunotherapy
… PI3KB inhibitor and ICB in PTEN-present tumors. Multiomics profiling revealed the upregulation of TNF-a signaling in PTEN-present tumors and the upregulation of genes associated with cell cycle, apoptosis, and immune response in PTEN-absent tumors after PI3Ka inhibition. In conclusion, our …
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Resolving developmental dynamics using single-cell sequencing data
… onto human data. Lastly, I present a single cell multiomics atlas of mouse skin development using transcriptomics and chromatin accessibility. This multimodal approach allows for a better description of cell states during skin development and homeostasis. I propose the existence of a slow and fast …
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Epithelial cell plasticity in chronic liver disease (MASLD/NAFLD)
… Finally, I employ single nuclei RNA and ATAC multiomics analysis to characterise in detail the in vitro process of cholangiocytes differentiation towards hepatocyte-like cells. Overall, the results propose that the diseased liver is exhibiting significant epithelial plasticity and uncover …
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The Use of Catalytically Dead Cas9 to Identify Key Transcription Regulators in Triple Negative Breast Cancer
… still remains a priority. With the advances of multiomics, many of the genes transcriptionally upregulated in TNBC have been identified, but how they are disregulated is still unknown: the understanding of how this works at a transcriptional level could contribute to the development of a novel …
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Somatic Mutation in Cancer and Healthy Human Tissue
… in cancer. Secondly, G&T-seq, a single cell multiomics technique, is extended to single nucleotide variant analysis. When applied to cortical neurons, it suggested a substantial level of technical artefacts in previously published mutational spectra. Thus, this multiomic approach offers an …
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Development of a framework for multiomic analysis in disease research
… studentship has highlighted the potentials for multiomics to improve disease research.
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Development of a ribosome profiling protocol to study translation in the yeast Kluyveromyces marxianus
… start site sequencing in what is described as “multiomics”. Using these multiomic data, the transcriptional and translational landscape is explored revealing a wide range of features including N-terminal extensions, upstream open reading frames and frameshifting. In addition, these data were …
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Multimodal, longitudinal, and mega-analysis of biomedical data
… that appropriately and efficiently represents multiomics data that is hierarchical, multimodal, and/or longitudinal in nature. A schematic of and methods for the data structure will be presented along with example usage to demonstrate how current challenges of alternative data structures are …
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Evaluating metabolism in human renal cancer using novel surgical models
… *in vivo* in surgical patients with RCC. Using a multiomics approach, I characterised the metabolic phenotypes of these tumours and for the first time, evidenced the suppression of gluconeogenesis *in vivo*. Secondly, by developing a novel *in situ* tissue sampling method, I demonstrated the …
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INTEGRATED SINGLE-CELL MUTATION, GENE EXPRESSION AND ISOFORM ANALYSIS TO DECONVOLVE ACUTE MYELOID LEUKEMIA HETEROGENEITY
… work, we have developed a novel high-throughput multiomics approach to integrate gene mutation, expression and isoform information at single-cell resolution. SCM-seq (Single Cell and Molecule sequencing) combines high- throughput droplet-based scRNA-seq to Nanopore single-molecule sequencing of …
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Oncogenic KRAS drives heterogeneity in metabolic and signalling pathways through genetic and non-genetic mechanisms
… cell genetics, single-cell biochemistry and a multiomics approach to investigate both genetic and non-genetic heterogeneity during initiation of PDAC. We generated an isogenic panel of cell lines harbouring the three most common mutations of KRAS in PDAC (G12D, G12R, G12V). This system …
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An investigation of the association between structural proteins and lipids with scalp hair curvature
… in the same group of hair samples using a multiomics approach to determine how they both relate to each other, and to hair curvature. After ethical approval, hair of varying curvature was collected from 90 participants and classified from straight to curly objectively (type I – VI) using a …
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Effects of system complexity on protein aggregation in neurodegeneration
Alzheimer's disease (AD) is a neurodegenerative disorder, the leading cause of dementia worldwide, and is one of the most pressing challenges in ageing societies. The disease is characterised by extensive brain atrophy, synaptic loss, and the accumulation of two types of protein aggregates: amyloid …
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