Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Multimer"”.
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CLINICAL AND LABORATORY CHARACTERISATION OF ACQUIRED VON WILLEBRAND SYNDROME
… with particular focus on bleeding phenotypes, multimer patterns, and underlying disease mechanisms. Methods: This cross-sectional study included patients diagnosed with AVWS between 2014 and 2025 at a single center. Clinical and laboratory data, including ISTH-BAT score, FVIII:C, VWF:Ag, …
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A Mutational Analysis of XcpQ, the Outer Membrane Component of the Type II Protein Secretion System From Pseudomonas Aeruginosa
… forms extremely stable pore-like outer membrane multimers, through which Type II secreted proteins are thought to pass. In this thesis I present a mutational analysis of the role of XcpQ in Type II protein secretion. A series of XcpQ mutants with single amino acid substitutions were identified by …
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Identification and Analysis of Germination-Active Proteins in Bacillus Spores
… crosslinking indicated that YpeB may exist as a multimer within the dormant spore. Further investigation of individual protein domains using bacterial two-hybrid analysis suggested that both N- and C-terminal domains of YpeB contribute to the formation of a multimer. In addition, the …
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The 2.7 resolution cryo-EM reconstruction of Mycobacterium tuberculosis encapsulin nanocompartment containing DyP peroxidase
… visualized. A model of both the Enc monomer and multimer was built, with comparisons to M. smegmatis in charge around the fivefold pore showing differences. The purification of Enc from M. tuberculosis was successful and a negative stain reconstruction of the nanocompartment was obtained. 2D …
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Investigating Potential Quantitative Trait Loci for Susceptibility to Experimental Autoimmune Encephalomyelitis in the BXD Family of Mice
… and chemokines, and distribution of the Fc multimer drug M019. Out of 16 strains tested, we identified 6 BXD strains susceptible to developing EAE, and found suggestive evidence of QTLs on chromosomes 5 and 11. We also found that the BXD43 strain expressed an extreme phenotype, categorized …
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Characterisation of high and low avidity peptide specific CD8+ T cells using immunologic, transcriptomic and proteomic tools
… specific CD8+ T cells, which was assessed my multimer staining. When these cells were subjected to in vitro stimulations with the target peptides, the functionality of the low avidity T cells was restored whereas the high avidity T cells failed to respond to lower peptide concentrations. This …
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Understanding the assembly of simple ssRNA virus nucleocapsids
… found to be largely unstructured and exist as a multimer, even at a concentration of 1 mg/mL, making crystallization attempts with it a vain and unprofitable goal. The nucleocapsid-like-particles produced with H158 or H124 both showed size heterogeneity within the reactions, and given the scale …
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Interaction Between the Metalloprotease ADAMTS-13 and the Proteins of the Alternative Pathway of the Complement System
Von Willebrand factor (VWF), a multimeric protein that has a central role in hemostasis, has been shown to interact with complement components. However results are contrasting and inconclusive. By studying 20 patients with congenital thrombotic thrombocytopenic purpura (cTTP) who can not cleave VWF …
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Molekulare und biochemische Charakterisierung des mitochondrialen Translationsaktivators Cbs2p in Saccharomyces cerevisiae
… bilden, sprechen die Daten dafür, das Cbs2p als Multimer in diesem Komplex vorliegt. Es konnte weiterhin gezeigt werden, dass der N-Terminus von Cbs2p eine essentielle Rolle bei der Homomerisierung des Proteins spielt. Die vorliegenden Ergebnisse erweitern das von Michaelis et al. (1991) …
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Computational Discovery of Bacterial Fibrillar Adhesins and Adhesive Domains
… methods were tested with the focus on AlphaFold Multimer. The results showed limitations in confidently predicting a binding target. The challenges encountered were discussed as well as an AlphaFold induced increase in the development of alternative methods tackling these challenges.
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Adaptive Immune Responses to Vaccination Against SARS-CoV-2 in Individuals with Immune Dysregulation
… obesity. The development of a nucleotide tagged multimer and monoclonal antibody validation system, enabled the identification of sequenced antigen specific B cells from individuals with severe obesity. In-depth analysis of the V(D)J sequences and differentiation states of these cells was …
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The Role of Autotaxin in Cancer
… protein 5 (FABP5). Modelling with AlphaFold-Multimer indicates FABP5 may interact with ATX at the opening to ATX’s lipid binding active site. Further investigation will be required to validate this idea.
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Synthese und Charakterisierung Cyclam-basierter Multimere als Basis für Radiopharmaka
… mit einer Vielzahl von Oberflächeneinheiten (Multimere) sind im Hinblick auf eine nuklearmedizinische Anwendung von besonderem Interesse, da durch definierte Strukturmodifizierung das Bindungsverhalten an bestimmte Zielstrukturen, die Löslichkeit und damit die Bioverteilung und Pharmakokinetik …
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Polypeptide (dis)assembly in bacterial spores and microalgae
… properties, necessitating the use of AlphaFold-multimer to evaluate the presence of protein complexes. An example is the six proteins in the GerP operon that moderate the response of a spore to factors that trigger germination. Another important complex to consider contains the proteins involved …
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Understanding the function and structure of herpes simplex virus type 1 pUL55, a novel immune evasion protein
… pUL55:pUL13 was modelled using AlphaFold2 Multimer. Point mutations predicted to disrupt the interaction from the model were incorporated expression plasmids and recombinant viruses. All tested mutations inhibited interaction between pUL55 and pUL13 and function of the pUL55:pUL13:pUS10 …
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Functions of RTF2 in DNA Replication and Checkpoint Control
… protein interaction screen using AlphaFold-Multimer. We identified a high-confidence interaction between RTF2 and checkpoint kinase 1 (CHK1), an essential kinase that functions downstream of ataxia-telangiectasia and Rad3-related (ATR) in the intrinsic S/G2 checkpoint, which we confirmed in …
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Multiplexed Mapping of the Interactome of G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins
… pulse-chase assay. We then used AlphaFold Multimer to predict the structure of the MRGPRX4-RAMP2 complex. The discovery that RAMP2 downregulates MRGPRX4 surface expression may have direct implications for future drug development for cholestatic itch. The application of the multiplexed SBA …
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Isolation and Use of the Aeromonas hydrophila Secretin ExeD for Nanopore Analysis
… these pores were non-native conformations of the multimer and had been incorrectly assembled. It was found that at low temperatures and low levels of IPTG, protein expression not only increased but the pores which were produced were uniform and had identical activity to one another. The …
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Significance of Protein Interactions in Mediating AF9 Function
… we show that AF9 is part of a protein multimer containing members of Polycomb group PRC1 complex, CBX8, RING1B, and BMI1. The interaction of AF9 with other PRC1 proteins is directly mediated by CBX8 and precludes AF9-DOT1L binding. Knockdown of CBX8 with shRNA leads to decreased ENaCα …