Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Multilocus sequence typing"”.
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Diversity and virulence of the genus Cronobacter revealed by multilocus sequence typing (MLST) and comparative genomic analysis
… in neonates and infants. In this study, a multilocus sequence typing (MLST) approach has been established to span the entire Cronobacter genus, by employing the alleles of 7 housekeeping genes (atpD, fusA, glnS, gltB, gyrB, infB and ppsA, total length 3036 bp). The 325 Cronobacter spp. …
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Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains
… C. saccharobutylicum strain groups respectively. Multilocus Sequence Typing (MLST) was developed for a smaller selection of strains and showed a further two strain groups within the NCP C. beijerinckii strains and three groups within the C. saccharobutylicum strains.
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Virulence potential and host response to Cronobacter sakazakii
… strains in the clonal complex 4 (CC4), including sequence type 4 (ST4), have been strongly associated with neonatal meningitis. In recent years, research on this organism has made substantial progress using improved identification and molecular methods including multilocus sequence typing. A …
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Novel Use of Scenarios in the Usability Engineering of a Next-generation MLST Tool
… process for the development of a next-generation multilocus sequence typing (MLST) tool. The following three research question were investigated during the usability process: (1) what are the differences in the elicited requirements as scenarios move further from extant work practices, (2) what …
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Comparative Whole-genome and Phenotype Analyses of Virulence Factors and Lethality in Nematode Challenge Assay among Aeromonas isolates
… strains isolated from various animals were sequenced and annotated in this study. Each isolate was further characterized using comparative genomic analyses and testing for production of selected virulence factors. Although previously identified as <em>Aeromonas hydrophila</em>, modern …
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Prevalence and control of Clostridium difficile in patients with cystic fibrosis
… toxin being detected in 31.66% of patients. Ribotyping of the C. difficile isolates revealed 16 distinct ribotypes, including the hyper virulent RT078. All isolates were sensitive to both Vancomycin and Metronidazole. The effect of CF and its treatment on the gut microbiota of CF patients was …
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Techniques for the identification and differentiation of Cronobactor species
… of all Cronobacter spp. Next, the DNA sequence-based method of multilocus sequence typing (MLST) was used to identify and characterize a collection of Cronobacter strains and these results were used in a variety of applications. These sequences were used to identify a novel species, …
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Typing and genome comparison of Neisseria meningitidis by DNA-microarrays
… polymorphism-directed oligonucleotide design for typing of N. meningitidis adopting the multilocus sequence typing (MLST) concept. The number of oligonucleotides needed to cover all known polymorphisms was much lower compared to the number needed if a tiling strategy would have been chosen. …
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Streptococcus equi subsp. zooepidemicus in New Zealand horses
… were shown to be genetically diverse via strain typing using either the single polymorphic gene, szp or standard multilocus sequence typing (MLST). Both typing schemes were shown to lack the discriminatory power needed to completely separate the isolates which was resolved by employing core …
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Enterococci and its enterocins as an alternative to antibiotics in poultry affected by necrotic enteritis
… resistome, virulome, plasmidome, toxin genes and multilocus sequence typing were analysed. The results showed that the tet genes (associated with tetracycline resistance) were the most common resistance genes detected and, interestingly, two isolates carried the erm(T) gene associated with …
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Pandemic <em>Vibrio parahaemolyticus</em>: Defining Strains Using Molecular Typing and a Growth Advantage at Lower Temperatures
… encountered in the environment. Molecular typing techniques; automated ribotyping, pandemic specific PCR and multilocus sequence typing (MLST), were compared to determine the best method for pandemic strain determination. MLST was the best method because it was the most informative and …
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Genomic diversity of Lactobacillus salivarius
… Comparative genomic hybridization (CGH) and multilocus sequence typing (MLST) were employed to assess the level of genomic diversity in L. salivarius. The wellcharacterised probiotic strains L. salivarius UCC118 was employed as a genetic reference strain. The group of test strains were chosen …
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Molecular Characteristics and Dissemination Mechanism of Vancomycin-Resistant Enterococci: A Tertiary vs. Non-Tertiary Health Care Hospitals
… genotype, structural analysis of Tn1546, multilocus sequence typing (MLST), virulence factor (esp), and dendrogram of repetitive extragenic palindromic PCR (rep-PCR) were analyzed in all VREF isolates. All the isolates harboured the vanA gene. 15 isolates (41.7%) from Non-Tertiaries and 13 …
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Diversity of acinetobacter baumannii isolates from Egypt
… blaOXA-71, blaOXA-78, blaOXA-94, blaOXA-89/100. Typing with Pulsed-field Gel Electrophoresis (PFGE) showed an overall similarity at only 28.69% between the isolates, with variation in pattern for isolates with similar blaOXA-51-like genes. Typing with Multilocus Sequence Typing (MLST) identified …
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Aster Yellows Phytoplasma, Aster Leafhopper And Canola: Development And Application Of Improved Molecular Methods For Pathogen Detection And Genetic Characterization Provide Increased Understanding Of Aster Yellows Disease
… a PCR-independent, hybridization-based multilocus sequence typing (MLST) assay was developed to precisely characterize phytoplasmas through the concurrent sequencing of seven taxonomic markers. This novel approach could serve as a standardized method for phytoplasma identification and …
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Determining role of bacteria associated with bacterial spot on pumpkin and squash fruit in the North Central Region of the United States
… test on pumpkin ‘Howden’ in a greenhouse. Both multilocus sequence typing (MLST) and restriction associated DNA sequence analysis (RADseq) were used to study genetic variation among X. cucurbitae isolates. Twenty X. cucurbitae isolates from Illinois, Indiana, Kansas, Ohio, and Wisconsin were …
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Antibiotic Resistance Profiles and Population Structure of Healthcare-associated and Community-associated Staphylococcus aureus isolated in Fort Portal, Western Uganda
… isolates.A population structure for the 41 sequenced isolates was inferred by comparing their core genomes. Twenty isolates formed a tight cluster corresponding to multilocus sequence typing (MLST) clonal complex (CC) 152, a CC found to be particularly prevalent in northern Africa. In …
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Brucellosis in Ethiopia: epidemiology and public health significance.
… goats with history of abortion were whole genome sequenced (WGS). Subsequent phylogenetic analysis involved whole genome SNPs,core-genome SNPs, and in silico analysis of Multilocus Sequence Typing and Multiple Loci Variable Number of Tandem Repeats to characterise and determine global clustering …
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CHARACTERISTICS OF CLINICAL NONTYPHOIDAL SALMONELLA ISOLATES FROM A HISTORICAL BIOBANK WITH A FOCUS ON THE INVASIVE SEROTYPE CHOLERAESUIS, LOMBARDY REGION (2001-2016)
… whole-genome sequencing (WGS), core-genome multilocus sequence typing (cgMLST), and in silico investigation of antimicrobial resistance determinants. Results. A total of 172 nontyphoidal serotypes (NTS) were identified. The most prevalent were S. Typhimurium (41.8%, n = 2,772), S. …
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