Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Multigene Family"”.
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Towards the cloning of the human heat shock protein twenty-seven multigene family
… of the human heat shock protein 27 (hsp 27) multigene family. Genomic DNA was obtained from human A549 lung cancer cells. Colony hybridization and the Polymerase chain reaction identified the hsp 27 genes. Positive clones were subcloned into pGEM-3Z. The DNA sequence information for three of …
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The role of the P28 multigene family in the pathogenesis of Ehrlichia chaffeensis infection.
… expresses a 28 kDa outer membrane protein from a multigene family known as P28/OMP-1. Different alleles from this multigene family are expressed in different host cell types, but the role of this membrane protein remains unknown. Also, few host cell receptors, as well as host-pathogen protein …
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Molecular genetic analysis of host resistance to soybean mosaic virus
… resistance sources. A heat shock protein (HSP) multigene family, HSP176L included, was analyzed for its positional proximity to the Rsv1 gene cluster. A technique termed amplified sequence length polymorphism (ASLP) was developed to convert known DNA sequences to PCR-based genetic markers. Among …
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The molecular basis of seed coat pigmentation in Glycine max
… related to CHS1 of the chalcone synthase (CHS) multigene family. Additionally, in many cases, mutations from the $i\sp i$ allele to the recessive i allele can be associated with the absence of a 2.3 kb Hind III CHS fragment which represents CHS4. Moreover, analysis of these mutations via PCR …
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Evolutionary and Paleobiochemical Analyses of Heme Peroxidases
… (TPO).These comprise a functionally diverse multigene family of enzymes associated with such diseases as asthma, Alzheimer’s disease and inflammatory vascular disease. This study has determined how the enzymes in the multigene family of MHP are related. The order of gene duplication events …
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Structure and expression of a U1 snRNA gene from Arabidopsis thaliana
… from pre-mRNA. U1 snRNA is encoded by a small multigene family in Arabidopsis, consisting of between five and eight U1 genes. U1a snRNA is unique in having an extra U residue at position +2, which occurs in the region of U1 RNA which base pairs with the 5' splice site of introns. Mutations in …
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Cloning and sequencing of a β-glucosidase of cDNA from Sorghum bicolor (L.) Moench and analysis of expression in seedlings
… showed that β-glucosidase is encoded by a small multigene family. Northern analysis data indicated that mRNA corresponding to the cloned gene is present at high levels in the node and mesocotyl 2 regions of the seedling and at low levels only in the zone of elongation region in roots. Other …
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Molecular characterization and induction of a peach beta-1,3-glucanase gene
… that $\beta$-1,3-glucanase gene belongs to a multigene family in peach.
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Isolation and Characterization of D-Myo-Inositol-3-Phosphate Synthase Gene Family Members in Soybean
… sequences suggested that GmMIPS1 is part of a multigene family of at least four similar members. The sequences of promoter and genomic regions of GmMIPS1 and GmMIPS2 revealed a high degree of sequence conservation. Northern and western blot analyses showed that MIPS transcript and protein are …
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The expression and regulation of pregnancy-specific glycoproteins in the mouse
… in the maternal bloodstream at term. This multigene family are immunoglobulin superfamily members and are predominantly expressed in the syncytiotrophoblast of human placenta and in giant cells and spongiotrophoblast of rodent placenta. PSGs are encoded by seventeen genes in the mouse and …
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Evolution of the Proteasome
Evolution of the proteasome multigene family was examined across all domains of life using experimental and computational techniques. First, degenerate primers designed to universally amplify all alpha proteasomes were used in the polymerase chain reaction with genomic DNA to probe the diversity of …
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Molecular Cloning of the cDNA for Porcine Parotid Hormone
… were derived from closely related genes-a multigene family. <em>In situ</em> hybridization and immunohistochemical staining of porcine tissues indicated that the acinar cells of the parotid glands are the primary location for both parotid hormone related mRNAs and the translation …
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MHC Class I diversity influences haematozoon infection intensity in the polymorphic black sparrowhawk (Accipiter melanoleucus)
The major histocompatibility complex (MHC) is a multigene family known to be the most variable gene group in vertebrates in terms of allelic diversity and gene number. Pathogen mediated selection is thought to be the major driving force behind the unusually high levels of MHC polymorphism in …
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Odorant receptors: regulation, signaling, and expression
… response. Odorant receptors comprise the largest multigene family identified to date, enabling the olfactory system to respond to an enormous pool of potential odorant molecules. This work focuses on the study of odorant receptors beyond the initiation of the primary olfactory response. The first …
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Studies on the optimization of expression and purification & Functional characterization of Class C – GPCRs
G-protein coupled receptor (GPCRs) is a multigene family consisting of more than 1000 genes. They are the most abundant membrane proteins found on a cell surface and are involved in several signaling pathways. In a cell, the signal is transduced by diverse activating endogenous ligands binding on …
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On Two Problems in Comparative Genomics of Eukaryotes
The recent advent of whole-genome sequencing allows us to use novel comparative methods to explore the genetic bases for traits of interest. Here, I present two case studies of such methods applied to eukaryote genomes. The first study regards the evolution of longevity in the mammalian proteome. …
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Understanding chicken BG genes at the RNA and protein levels
… BG system is a highly polymorphic and polygenic multigene family encoding type I transmembrane proteins, with butryophilins as homologues in mammals, some of which are crucial in T cell regulation. There are three genomic locations where BG genes are found: one singleton BG gene (BG0) on …
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Molekularbiologische und funktionelle Charakterisierung neuer "Chicken Ig-like" Rezeptoren
… thesis seven new members of this receptor family were identified in the chicken. All receptors identified were located on one single clone of a chicken BAC library, which indicates that they are tightly clustered on the chromosome. This localisation and the high amino acid homology …
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Understanding antigen processing in chickens using genome editing technology
… limited polymorphism, providing peptides for a multigene family of well-expressed classical MHC molecules. Outside of mammals, the chicken is the only system in which peptide loading and presentation has been studied in molecular detail. Compared to mammals, the chicken MHC is small, simple and …
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Molecular evolution of beta (â) keratins in three bird species and divergence time estimates for the avian â-keratin subfamilies
… In the avian lineage, the â-keratins make up a multigene family containing at least four major subfamilies (scale, claw, feather-like and feather â-keratins). Three avian genomes, the chicken (Gallus gallus), zebra finch (Taeniopygia guttata) and the turkey (Meleagris gallopavo), have recently …
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