Global ETD Search
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Showing 1 to 11 of 11 for “"Muc5b"”.
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The biochemical analysis of mucus and mucins in respiratory diseases with a focus on tuberculosis
… of this layer of mucus are mucins. MUC5AC and MUC5B are the predominant respiratory tract mucins. However, little is known of the association between respiratory mucins and TB. This study aimed at describing the types and role of respiratory mucins in TB. Fifty six sputum samples, 17 tracheal …
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The role of crude saliva and purified salivary mucins in the inhibition of the Human Immunodeficiency Virus type 1
… pH 6.5. Sepharose CL-4B gel filtration separated MUC5B and MUC7 in saliva. Mucins were purified by density gradient ultra-centrifugation in caesium chloride. Western blotting and amino acid analysis determined the size, charge and identity of the mucins. The inhibitory activity of crude saliva, …
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The effect of reduction, trypsin digestion and de-glycosylation of salivary mucins in the inhibition of human immunodeficiency virus type 1
… that crude saliva and purified salivary mucins (MUC5B and MUC7) inhibit HIV-1 infection in vitro. However, it is not known whether the specific arrangement of mucin carbohydrate residues is important for mucin interactions with HIV-1, or if the negative charge afforded by sialic acid and sulfated …
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Role of salivary films in assessing astringent compounds
… containing a galloyl ring, interacts with MUC5B to a much lower extent than with PRPs. However, this interaction is enough to alter and enhance the binding of MUC5B and other salivary proteins shown to aggregate with polyphenols in pellicle models. This response was echoed with EGCG washes …
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The biochemical and molecular characterisation of respiratory mucins in TB
… of the dominant respiratory mucins (MUC5AC and MUC5B) and MUC2 has been investigated in chronic airway diseases as it is the mucin glycoprotein that confers upon mucus its biological, rheological and physicochemical properties. Within South Africa, specifically the Western Cape, TB has wreaked …
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Vesicle-associated Membrane Proteins in Airway Mucin Secretion
… the subcellular level, VAMP8 is colocalized on MUC5B-only, MUC5AC-only, and MUC5B/MUC5AC double-positive granules. To identify the VAMP predominately responsible for baseline secretion, we focused on VAMP2 and VAMP3 based on their high expression levels in airway secretory cells and roles in …
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Candidate genes other than the CFTR gene as possible modifiers of pulmonary disease severity in cystic fibrosis
… and GCLC repeats, 4) mucin genes (MUC2 and MUC5B). Pulmonary disease progression and survival in patients with chronic Burkholderia cepacia complex (BCC) infection were also investigated controlling for genomovar and RAPD type of the organism. BCC infection was associated with more severe …
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Packaging and Secretion of Airway Mucins
… 1) Are the polymeric mucins MUC5AC and MUC5B packaged in the same or separate granules? 2) What components of the exocytic machinery on mucin granules interact with the plasma membrane machines, and can these be used as markers to define mucin trafficking prior to secretion?</p> <p>3) …
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Deciphering the mechanism of foxa2 inactivation by respiratory pathogens in chronically diseased lungs
… of airway mucin5ac (MUC5AC) and mucin5b (MUC5B) genes. In diseased human and other mammalian airways, FOXA2 is inactivated in both club cells and ciliated cells, resulting in the differentiation of these pluripotent airway cells into the mucin secreting goblet cells. Mucin-containing mucus …
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INVESTING THE ROLES OF E26 TRANSFORMATION-SPECIFIC HOMOLOGOUS FACTOR IN CYSTIC FIBROSIS LUNG DISEASE
… absence of EHF led to a decreased expression of MUC5B in organoids and alterations in the transcriptomic signature of Basal Cell (BCs) in ALI cultures. Finally, we assessed physiological functions (trans-epithelial electrical resistance (TEER), cilia motility, and cell migration) and functions …
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Syntaxin-3 mediates baseline and stimulated mucin secretion
<p>There are two rates at which airway epithelial cells secrete mucin. The low baseline rate accounts for ciliary clearance, and the high stimulated rate obstructs airways in protection from helminths or in obstructive pulmonary diseases. The exocytosis of mucins occurs via the four-helix SNARE …