Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Muc5ac"”.
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Expression of cysteine-rich pig gastric mucin (Muc5AC) domains in Pichia pastoris and their pH-dependent properties
… (Cys-Rep) domains of the PGM mucin gene Muc5AC. These were cloned into the vector pDEST911 and transformed into the yeast expression host Pichia pastoris. The secreted recombinant peptides were purified from culture supernatant by nickel affinity chromatography yielding 5-15 mg/L. The …
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Dissecting the Relationship between Mucin 5AC and the Lung/Gut Microbiome in the Pathogenesis of K-ras Mutant Lung Cancer
… tumors harbor high expression of Mucin 5 AC (MUC5AC), a main airway secretory mucin, have a poor prognosis and significantly lower survival rate. Additionally, evaluation of MUC5AC-deletion in our murine model of KM-LUAD demonstrated a significant reduction in tumor burden. Given the known …
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The biochemical analysis of mucus and mucins in respiratory diseases with a focus on tuberculosis
… constituents of this layer of mucus are mucins. MUC5AC and MUC5B are the predominant respiratory tract mucins. However, little is known of the association between respiratory mucins and TB. This study aimed at describing the types and role of respiratory mucins in TB. Fifty six sputum samples, 17 …
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The biochemical and molecular characterisation of respiratory mucins in TB
The role of the dominant respiratory mucins (MUC5AC and MUC5B) and MUC2 has been investigated in chronic airway diseases as it is the mucin glycoprotein that confers upon mucus its biological, rheological and physicochemical properties. Within South Africa, specifically the Western Cape, TB has …
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Vesicle-associated Membrane Proteins in Airway Mucin Secretion
… level, VAMP8 is colocalized on MUC5B-only, MUC5AC-only, and MUC5B/MUC5AC double-positive granules. To identify the VAMP predominately responsible for baseline secretion, we focused on VAMP2 and VAMP3 based on their high expression levels in airway secretory cells and roles in exocytosis in …
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Effects of vitamin D on inflammation and oxidative stress in airway epithelial cells
… expression in the primary cells and reduced MUC5AC mRNA and protein expression in the mucous producing NCI-H292 cells. Interestingly, vitamin D treatment gave a biphasic effect on induction of inflammatory mediators by TNFα in the NCI-H292 cells. Vitamin D also modulated the induction of …
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Packaging and Secretion of Airway Mucins
… and secretion. 1) Are the polymeric mucins MUC5AC and MUC5B packaged in the same or separate granules? 2) What components of the exocytic machinery on mucin granules interact with the plasma membrane machines, and can these be used as markers to define mucin trafficking prior to …
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Deciphering the mechanism of foxa2 inactivation by respiratory pathogens in chronically diseased lungs
… as a transcription repressor of airway mucin5ac (MUC5AC) and mucin5b (MUC5B) genes. In diseased human and other mammalian airways, FOXA2 is inactivated in both club cells and ciliated cells, resulting in the differentiation of these pluripotent airway cells into the mucin secreting goblet cells. …
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Pathogenesis of Dry Eye in Graft Versus Host Disease (GVHD): Role of Ocular Mucins and Conjunctival Fibrosis
… a decrease in ocular surface mucins, MUC4 and MUC5AC and loss of conjunctival goblet cells. Our data showed that topical treatment by mucin secretagogue, rebamipide, partially attenuates GVHD-associated damage to ocular surface. Hyperosmolar tears are a consistent feature of dry eye disease. …
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Syntaxin-3 mediates baseline and stimulated mucin secretion
<p>There are two rates at which airway epithelial cells secrete mucin. The low baseline rate accounts for ciliary clearance, and the high stimulated rate obstructs airways in protection from helminths or in obstructive pulmonary diseases. The exocytosis of mucins occurs via the four-helix SNARE …
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MUC13 Enhances Colorectal Cancer Metastasis
… Genetic variations in mucins such as MUC1 and MUC5AC have been found to increase the risk of stomach cancer and certain Allele mutations nearly doubling that risk in. We investigated if MUC13 had any genetic variants and found 5 different splice variants. Three of those splice variants were …