Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Mre11/Rad50"”.
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THE FUNCTION OF MRN (MRE11-RAD50-NBS1) COMPLEX DURING WRN (WERNER) FACILITATED ATM (ATAXIA-TELANGIECTASIA MUTATED) ACTIVATION
… with replication fork collapse. Because the Mre11-Rad50-Nbs1 (MRN) complex, a sensor of DSBs, is known to interact with WRN and ATM, we investigated whether the MRN complex mediates the WRN-dependent ATM pathway activation. In this study, we employed short-hairpin RNA to generate WRN- and …
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DNA double-strand break repair studied by atomic force microscopy
… most lethal type of DNA damage. The conserved Mre11-Rad50 DNA repair complex enables the catalytic activities of the Mre11 nuclease and the Rad50 ATPase to function together to coordinate the recognition and processing of DSBs prior to the recruitment of long-range end-resection machinery …
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RAD51-MEDIATED ABASIC SITES PROTECTION PREVENTS REPLICATION FORK COLLAPSE.
… forks onto which RAD51 binds preventing MRE11-RAD50 nuclease-mediated AP site cleavage. Additionally, our results demonstrate that Polymerase Theta (θ) is involved in filling in AP sites over genomic DNA regions containing ssDNA gaps, thereby suppressing MRE11-RAD50 nuclease-dependent …
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Mechanisms and regulation of dsDNA break repair in the Sulfolobus genus of thermophilic archaea
… all domains of life. One such component is the Mre11/Rad50 complex that is involved in the initial recognition of damage and recruitment of subsequent repair factors. Understanding the function of this DNA repair complex and any associated proteins has implications for human cancers and aging. …
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Mechanisms of Palindrome-Mediated Chromosomal Instability
… and these hairpins are cleaved by the Mre11/Rad50/Xrs2/Sae2 complex. In contrast, palindromic sequences with spacers longer than 8 bp are not cleaved by MRX/Sae2 and do not block replication fork progression. I show that these effects are mediated by single stranded DNA binding protein …
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The interaction of CtIP with DNA damage response proteins
… damage sensor proteins such as the MRN complex (Mre11, Rad50 and NBS1) and RPA70, signal transducer proteins such as the PIKK kinases ATM, ATR and SMG1 and the mediator proteins 53BP1 and MDC1. All of those proteins are involved in detecting and repairing double stranded or single stranded …
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Single-molecule studies reveal mechanisms of human DNA double-strand break repair
… pathways: the Ku70-Ku80 heterodimer (Ku) and the Mre11-Rad50-Nbs1 complex (MRN), respectively. Ku encircles the DNA ends and recruits other factors, such the kinase DNA-PKcs, to bluntly ligate the ends back together. In contrast, MRN along with the long-range nuclease Exo1 and helicase BLM digests …
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Prediction and Investigation of Novel Proteins in DNA Double Stranded Break Repair
… initiates and stabilizes the DNA ends, Mre11/Rad50/Xrs2 brings broken ends to close proximity and Dnl4/Lif1/Nej1 ligates the DNA ends. Protein-protein interaction (PPI) has been utilized in functional genomics studies to identify novel proteins involved in different pathways based on …
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Mechanism of Mismatch Repair Induced Mutagenesis in Somatic Hypermutation
… In addition, I explore the role of the Mre11-Rad50-Nbs1 complex in its contribution to A:T mutations in Ramos cells. Taken together, these studies demonstrate that conversion of classical DNA repair pathways into mutation-generating processes is driven by the unique environment of the V …
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Widespread Phosphorylation of H2AX by Adenovirus Requires Viral Genome Replication and the E1B-55k or E4orf3 Proteins
… members of the sensor complex composed of Mre11/Rad50/NBS1 for degradation and relocalization, as well as targeting the effector protein DNA ligase IV. Despite inactivation of these cellular sensor and effector proteins, infection results in high levels of histone 2AX phosphorylation, or …
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Novel functions of N-acetyltransferase 10 (NAT10) in DNA repair and replication with potential implications for premature ageing syndromes
… of NAT10 with the complex of three proteins: MRE11, RAD50 and NBS1/NBN (MRN complex). This complex plays an important role in the sensing and repair of DNA double-strand breaks (DSBs), highly genotoxic lesions that can result in unrestrained cellular lethality or genomic instability. During …
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Characterization of a Novel 53BP1-Dependent Mechanism that Promotes Non-Homologous End Joining of Deprotected Telomeres by Increasing Chromatin Mobility
… fusions. We have examined the roles of the Mre11/Rad50/NBS1 (MRN) complex, H2AX, MDC1, and 53BP1 in the NHEJ of dysfunctional telomeres. We have demonstrated that among these factors, 53BP1 is required for the fusion of telomeres, whereas the MRN complex, H2AX, and MDC1 only stimulate the …
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Molecular Mechanisms of Mitotic Spindle Assembly and Accurate Chromosome Segregation
… and mammalian cells. I show that the MRN (Mre11, Rad50, and Nbs1) complex is required for metaphase chromosome alignment. Consistent with the result of my colleague using Xenopus egg extracts, disruption of MRN function by depleting Mre11 using an inducible shRNA system, or Mre11 inhibitor …
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How is Ataxia-Telangiectasia Mutated Protein Kinase Activated?
… breaks in DNA. This damage is detected by the Mre11-Rad50-Nbs1 (MRN) complex, which recruits ATM to the DNA. Upon activation, ATM phosphorylates a vast range of substrates, which triggers a multitude of downstream pathways such as DNA damage repair, cell cycle arrest, senescence and sometimes …
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Estudio de la ubicuitilación del complejo MRN mediada por SCF(bTrCP/FBXW7): implicaciones biológicas
… las roturas de doble cadena es el complejo MRN (MRE11, RAD50 y NBS1), esencial para la identificación de las roturas, el reclutamiento de diversos factores de señalización y el procesamiento, todo ello encaminado a la reparación de las mismas. El complejo MRN también interviene en otros procesos, …
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Distinct DNA Damage Signaling in the Brain Distinguishes ATLD, NBS, and ATR-Seckel Syndrome
… nervous system. Hypomorphic mutations in the Mre11/Rad50/NBS1 complex (MRN complex), which is required for efficient ATM activity, result in distinct syndromes. Ataxia-telangiectasia-like disease (ATLD) results from truncating mutations in Mre11 and presents with neurodegeneration similar to …