Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"Mre11"”.
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THE FUNCTION OF MRN (MRE11-RAD50-NBS1) COMPLEX DURING WRN (WERNER) FACILITATED ATM (ATAXIA-TELANGIECTASIA MUTATED) ACTIVATION
… with replication fork collapse. Because the Mre11-Rad50-Nbs1 (MRN) complex, a sensor of DSBs, is known to interact with WRN and ATM, we investigated whether the MRN complex mediates the WRN-dependent ATM pathway activation. In this study, we employed short-hairpin RNA to generate WRN- and …
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DNA double-strand break repair studied by atomic force microscopy
… most lethal type of DNA damage. The conserved Mre11-Rad50 DNA repair complex enables the catalytic activities of the Mre11 nuclease and the Rad50 ATPase to function together to coordinate the recognition and processing of DSBs prior to the recruitment of long-range end-resection machinery …
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Chemosensitization of Hepatocellular Carcinoma to Gemcitabine By Non-Invasive Radiofrequency Field-Induced Hyperthermia
… degrades and inhibits localization of Mre11 to gemcitabine-stalled replication forks. Furthermore, gemcitabine-treated cells that were also treated with hyperthermia demonstrate a prolonged passage through late S/ G2 phase of cell cycle in comparison to cells treated with gemcitabine …
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Caretaker-Gen-Syndrome
… dessen Translationsprodukt im Komplex mit MRE11 und RAD50 eine zentrale Rolle in DNA-DSB-Reparatur und Zellzykluskontrolle spielt. Weitere Mutationen bei Patienten mit NBS-ähnlichem Phänotyp wurden im Gen der DNA-Ligase IV identifiziert, die zusammen mit weiteren Angehörigen des …
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Distinct DNA Damage Signaling in the Brain Distinguishes ATLD, NBS, and ATR-Seckel Syndrome
… nervous system. Hypomorphic mutations in the Mre11/Rad50/NBS1 complex (MRN complex), which is required for efficient ATM activity, result in distinct syndromes. Ataxia-telangiectasia-like disease (ATLD) results from truncating mutations in Mre11 and presents with neurodegeneration similar to …
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Effect of TIS21 on DNA Damage Repair Induced by Etoposide
… PRMT1 activity and induced hypermethylation of Mre11 protein. In conclusion, TIS21 involved DNA damage signaling and inhibited Chk2-p53 pathway via Mre11 methylation.
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Molecular Mechanisms of Mitotic Spindle Assembly and Accurate Chromosome Segregation
… and mammalian cells. I show that the MRN (Mre11, Rad50, and Nbs1) complex is required for metaphase chromosome alignment. Consistent with the result of my colleague using Xenopus egg extracts, disruption of MRN function by depleting Mre11 using an inducible shRNA system, or Mre11 inhibitor …
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RAD51-MEDIATED ABASIC SITES PROTECTION PREVENTS REPLICATION FORK COLLAPSE.
… forks onto which RAD51 binds preventing MRE11-RAD50 nuclease-mediated AP site cleavage. Additionally, our results demonstrate that Polymerase Theta (θ) is involved in filling in AP sites over genomic DNA regions containing ssDNA gaps, thereby suppressing MRE11-RAD50 nuclease-dependent …
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Prediction and Investigation of Novel Proteins in DNA Double Stranded Break Repair
… initiates and stabilizes the DNA ends, Mre11/Rad50/Xrs2 brings broken ends to close proximity and Dnl4/Lif1/Nej1 ligates the DNA ends. Protein-protein interaction (PPI) has been utilized in functional genomics studies to identify novel proteins involved in different pathways based on …
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The evaluation of targeted radionuclide therapies and radio sensitising agents in malignant melanoma
… tumour specificity and tumour growth delay. The MRE11 inhibitor Mirin produced cytoxicity in vitro but not in vivo when combined with 2Gy X-ray radiation, reducing but not inhibiting γH2AX foci clearance post radiation treatment. The novel IKKβ Inhibitors SU567 and SU182 produce effects …
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Widespread Phosphorylation of H2AX by Adenovirus Requires Viral Genome Replication and the E1B-55k or E4orf3 Proteins
… members of the sensor complex composed of Mre11/Rad50/NBS1 for degradation and relocalization, as well as targeting the effector protein DNA ligase IV. Despite inactivation of these cellular sensor and effector proteins, infection results in high levels of histone 2AX phosphorylation, or …
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Mechanisms and regulation of dsDNA break repair in the Sulfolobus genus of thermophilic archaea
… all domains of life. One such component is the Mre11/Rad50 complex that is involved in the initial recognition of damage and recruitment of subsequent repair factors. Understanding the function of this DNA repair complex and any associated proteins has implications for human cancers and aging. …
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Novel functions of N-acetyltransferase 10 (NAT10) in DNA repair and replication with potential implications for premature ageing syndromes
… of NAT10 with the complex of three proteins: MRE11, RAD50 and NBS1/NBN (MRN complex). This complex plays an important role in the sensing and repair of DNA double-strand breaks (DSBs), highly genotoxic lesions that can result in unrestrained cellular lethality or genomic instability. During …
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Analysis of Meiotic Cancer Testis Antigens and Their Contribution to Tumorigenesis
… degradation, an event which is mediated by the MRE11-DNA2-BLM pathway. Moreover, following exogenous induction of DNA replication stress, HORMAD1 deleted cells accumulate single stranded DNA (ssDNA). We find that these phenotypes are the result of a lack of RAD51 and BRCA2 loading onto stalled …
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Zur Funktion des MPH1-Gens von Saccharomyces cerevisiae bei der rekombinativen Umgehung von replikationsarretierenden DNA-Schäden
… Interaktionen von mph1 mit apn1, apn1 apn2, mre11, sgs1, top3 und rad54 führten zu der Hypothese, dass Mph1 an einem fehlerfreien Weg zur Umgehung von DNA-Schäden, die den Fortgang der DNA-Replikation verhindern, beteiligt ist. In Abwesenheit von Mph1 werden diese DNA-Läsionen fehlerbehaftet …
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Γενετική ανάλυση στον κληρονομικό καρκίνο του μαστού
… TP53, PTEN, STK11, CDH1, ATM, BARD1, MLH1, MRE11, MSH2, MSH6, MUTYH, NBN, PMS1, PMS2, RAD50, RAD51C) με την χρήση τεχνολογίας αλληλούχισης επόμενης γενεάς. Για το σκοπό αυτό αναλύθηκαν 42 ασθενείς με σοβαρό ιστορικό καρκίνο μαστού ή/και ωοθηκών, οι οποίες είχαν βρεθεί αρνητικές για …
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Dynamics of DNA-repair factors and chromosomes studied by laser-UVA-microirradiation and laser-photobleaching
… of irradiated cells had accumulations of Mre11 protein already 5 - 10 minutes after irradiation. This is consistent with reports that nuclear Mre11 foci appeared early in the response to ionizing radiation, but absolute response times were faster after microirradiation than after ionizing …
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The Crosstalk Between DNA Mismatch Repair and Replication
… and BRCA1 to replication forks, and promotes MRE11-catalyzed nascent strand degradation. This MMR-dependent degradation causes DNA breaks and chromosome abnormalities, thereby promoting an ultramutator phenotype. Therefore, our findings identify a novel MMR function in triggering replication …
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Mechanisms of Palindrome-Mediated Chromosomal Instability
… and these hairpins are cleaved by the Mre11/Rad50/Xrs2/Sae2 complex. In contrast, palindromic sequences with spacers longer than 8 bp are not cleaved by MRX/Sae2 and do not block replication fork progression. I show that these effects are mediated by single stranded DNA binding protein …
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Understanding The Repair Mechanisms at Ionizing Radiation-induced Damage in The Human Genome
… of XRCC1 with the end resection enzymes Mre11 and CtIP. The XRCC1 immunocomplex isolated from U2OS cells had Alt-EJ activity in vitro; this activity was significantly higher in the immunocomplex from pre-irradiated cells. Our studies thus suggest that activation of Alt-EJ proficient …
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