Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Mps1"”.
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Chromosomal instability and tumorigenesis : genetic analysis of the murine spindle checkpoint gene Mps1
… mutant allele of the spindle checkpoint kinase MPS1 (MpslA) and induced its expression in thymocytes. I show that MpslA is a partial loss of function allele for spindle checkpoint activation which results in chromosome missegregation in MEFs and embryonic lethality in mice. Expression of MpslA …
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Safeguard of Mitosis: The Spindle Checkpoint
… of a checkpoint kinase, monopolar spindle 1 (Mps1), in both attachment sensing and checkpoint signaling. Of kinetochore proteins, the KMN network acts as both a critical microtubule receptor and a signaling platform for the spindle checkpoint. The human KMN contains the kinetochore null 1 …
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Molecular Mechanisms of Mitotic Checkpoint Complex Assembly onto Kinetochores
… onto the outer kinetochore by means of a Mps1-dependent phosphorylation cascade, which creates a catalytic platform for MCC assembly. This thesis investigates several mechanisms of catalytic MCC assembly in humans using a combination of biochemical assays and structural biology. Chapter 3 …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… complex. The roles of two kinases- BubR1 and Mps1, implicated in spindle assembly checkpoint were investigated. A checkpoint complex containing BubR1 and Bub3 has been purified from mitotic human cells. BubR1 directly interacts with Cdc20 and inhibits the activity of APC in vitro,much more …
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Generating the Spindle Assembly Checkpoint Signal at the Kinetochore
… complex. The roles of two kinases- BubR1 and Mps1, implicated in spindle assembly checkpoint were investigated. A checkpoint complex containing BubR1 and Bub3 has been purified from mitotic human cells. BubR1 directly interacts with Cdc20 and inhibits the activity of APC in vitro,much more …
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Funktionelle Charakterisierung und Positionierung von Drosophila Cenp-C im Zentromer/Kinetochor-Komplex
… des Spindelkontrollpunktproteins Mps1 zu charakterisieren. Zudem wurde das Verhalten von Cenp-C, der Ndc80-Komplex-Komponente Nuf2 und Mps1 als Antwort auf eine beschädigte Spindel nach Sauerstoffentzug untersucht.
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Functions of RTF2 in DNA Replication and Checkpoint Control
… checkpoint protein, monopolar spindle kinase 1 (MPS1), which monitors microtubule-kinetochore connection in the spindle association checkpoint (SAC). RTF2 and MPS1 share extensive co-dependency overlap in cancer cell lines and interact by immunoprecipitation. RTF2- deficient cells exhibit …
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Dissecting the Spatiotemporal Regulation of Yeast Kinetochore Interactions
… Cnn1 localization was proposed to depend on Mps1 kinase activity at Cnn1-S74 based on in vitro experiments demonstrating the Cnn1-Ndc80 complex interaction. We demonstrate that in G1 through metaphase, Cnn1 localizes via the histone-fold domain or a N-terminal Spc24/25-
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Exploring the effects and efficacy of novel cell cycle regulating compounds in aggressive Breast, Ovarian and Cervical Cancer
… compounds Reversine (an Aurora kinase B and Mps1 inhibitor) and Apcin (an APC/C inhibitor) have been established to be cytotoxic to cancer. This thesis aimed to confirm the efficacy of these compounds and to test the cytotoxicity of three novel small molecules that interfere with cell cycle …
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Examining the Regulation of Cell Division by the Spindle Assembly Checkpoint
… recruitment of Mad1. I show that the kinases Mps1 and Aurora B are necessary for the maintenance of checkpoint arrest, independently of their other "upstream" checkpoint functions. I also show that localization of Mad1 at other chromosomal locations is not per se sufficient to induce …
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Exploration of Cell Cycle-Specific Essential Gene Functions in the Microbial Plant Chlamydomonas Reinhardtii
… subunits, and the mitotic kinases Aurora B and MPS1. The phenotype of the cdka-1 mutant suggested a specific role for CDKA in the transition from cell growth to initiation of the S/M cell division program. CDKB, in contrast, functions specifically after DNA replication, in entry into the first …