Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Mouse Sperm"”.
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Re-localization of SNARE proteins in mouse sperm prior to the acrosome reaction
… of the SNARE family, syntaxin, is found on the sperm plasma membrane while synaptobrevin, is found on the outer acrosomal membrane. During the sperm acrosome reaction, the outer acrosomal membrane fuses at hundreds of points with the overlying plasma membrane, resulting in release of the …
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Characterization of the lipid composition of washed and percoll gradient centrifuged epididymal mouse sperm.
… process during which the fluidity of the sperm membrane increases to prepare sperm for the acrosome reaction (AR) and subsequent sperm-egg binding. While cholesterol efflux may be partially responsible for this increase, modification of lipid components, such as remodeling of specific …
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Characteristics of sperm binding to the mouse oviduct
A wide variety of animals store sperm between mating and ovulation. The sperm reservoir prolongs sperm lifespan and keeps sperm in a quiescent stage due to delayed capacitation and suppressed motility. The objective of this study was to determine different characteristics of sperm binding to the …
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Phage Display to Identify Peptides Binding to or Penetrating the Mouse Zona Pellucida
… phage display techniques, which bind sites on mouse embryos, ovaries, cytoplasmic membranes and/or intracytoplasmic components. Specifically, M13 coliphage 7-mer, 12-mer and 15-mer random peptide libraries were used separately for biopanning. Peptides derived from the amplified pools were …
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Expanding the genome editing toolbox for biomedical applications
… may go undetected. To this end, we targeted two mouse genes, Ace2 and Foxg1, via CRISPR-READI, and generated three GM mouse lines. Next, we employed Nanopore-Cas9-targeted sequencing (nCATS) to analyze the targeted sites and observed concatemer formation, along with unintended insertion of AAV’s …
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Towards A Unified Model Of Sperm Chromatin Structure
<p>Sperm possess several layers of information that are delivered to the oocyte alongside the paternal DNA. Examples of potential sperm borne molecular cues of probable use to the embryo include RNAs and local and global chromatin structure. To identify candidate sperm RNAs that likely reach the …
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Regulation of SNARE function during sperm capacitation to promote the acrosome reaction
… frequent molecular causes of the inability of sperm to undergo acrosomal exocytosis. In the current study, my goal was to develop a clearer understanding of how the acrosome reaction is regulated during capacitation so that the molecular defects that cause infertility can be identified. I …