Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Mouse Genetics"”.
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Technology development in mouse genetics and epigenetics
… in biological research cannot be overstated. The mouse in particular has been very useful in understanding questions in many areas of research such as developmental biology, cancer biology, neuroscience and genetics. However, even though the methods to make transgenic mice and gene knockin and …
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Hdac3 is a Critical Regulator of Neural Crest Progenitor Cell Biology
… means. In this dissertation, I use mouse genetics to explore the previously undescribed role of Hdac3 in regulating neural crest progenitor cell behavior. By genetically deleting Hdac3 in premigratory neural crest cells, I use in vivo and ex vivo techniques to show that Hdac3 plays …
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Exploring a behavioral role for presynaptic inhibition at spinal sensory-motor synapses
… if any, to motor behavior. Here we have used mouse genetics to gain access to the set of GABAergic interneurons that provide direct input to sensory terminals, and show that their activation evokes the defining physiological features of presynaptic inhibition. Genetic ablation of this set of …
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Uncovering Signalling Pathways Mediating Gastrointestinal Morphogenesis and Tumorigenesis
… mesenchyme. Moreover, by using a combination of mouse genetics, live imaging and 3D-culture based approaches, I have demonstrated a requirement for planar cell polarity in instructing organized cell migration for mesenchymal clustering, which is critical for each emerging villus. Therefore, …
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Building a Methodological Framework for Cell Fate Engineering
… Gene regulatory network analysis and mouse genetics identify and confirm key nodes mediating DLX2-dependent fate reprogramming. Therefore, this study dissects the gene programs of in vivo reprogramming with single-cell transcriptomics and paves the way for applying Reprogram-seq in …
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Biochemical and Genetic Studies of Beclin 1 Function in Autophagy
… and degeneration. In a study that combines mouse genetics and biochemistry, three novel Beclin 1 interaction proteins Atg14L (yeast Atg14-like), Rubicon (RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein) and Nrbf2 were identified from mouse tissues. Gel filtration …
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Studying the Effect of TBX4 Loss-of-Function on Postnatal Lung Development and How it Predisposes to Pulmonary Hypertension
… patients. Therefore, in this project, using mouse genetics, we sought to disrupt Tbx4 expression and investigate if there is consistency with the diseases observed in humans. We found that Tbx4 mutant lungs have increasing alveolar simplification as confirmed by mean linear intercept (MLI) at …
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Rescue of Fragile X Syndrome phenotypes in Fmr1 KO mice by the small molecule PAK inhibitor FRAX486
… basis of FXS allowed for the development of a mouse model of autism. The fmr1 knockout (KO) mouse displays phenotypes similar to symptoms in the human condition - including hyperactivity, repetitive behaviors, and seizures. Humans and mice share not only behavioral expression of the disease, …
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Endosomal membrane dynamics underlying cell spreading: A role for the small GTPase Arf6
… Arf6 to study its cellular function, we employed mouse genetics. In this system, mouse embryonic fibroblasts (MEFs) were derived and immortalized from mice genetically manipulated for the acute deletion of Arf6 using a tamoxifen inducible Cre/loxP recombination system. Acute deletion of Arf6 in …
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Molecular Mechanisms and Antigen Receptor Requirements for Lymphocyte Adaptation to Intestinal Tissues
… of the gut epithelium on T cells by integrating mouse genetics with single-cell RNAsequencing analyses. Transcriptionally, CD4+ T cells from mLN, LP and IE segregate based on the intestinal layer they occupy; trajectory analysis suggests a stepwise loss of CD4-programming and acquisition of an …
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Integration of ATM, ATR, and DNA-PKcs Signaling Maintains Genome Integrity During Neurogenesis
… system development.</p> <p>Here we utilized mouse genetic analyses to identify the functional interplay between these kinases during neurogenesis. DNA-PKcs function is directly involved in and most associated with the non-homologous end-joining (NHEJ) pathway. The function of DNA-PKcs during …
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Regulatory dynamics of transcription factors TBX18 and WT1 in mouse prostate development
… and ATAC-seq from embryonic day 18.5 (E18.5) mouse urogenital sinus (UGS), with the goal of identifying elements that interact with the Tbx18 promoter in developing prostate. The data revealed dozens of active chromatin elements distributed throughout a 1.5 million base pair topologically …
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DISSECTING THE ROLE OF Β-CATENIN AND MYC IN GASTRIC TUMOR FORMATION
… elusive. Here, using conditional transgenic mouse models, we show that Wnt constitutive activation does not have any oncogenic effect in the gastric epithelium and cooperates with MYC in driving gastric tumorigenesis. By integrating transcriptomic and epigenomic analysis with organoid models, …
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Functional Analysis of Daxx In Tumorigenesis of Pancreatic Neuroendocrine Tumors and Embryonic Development
… The loss of Daxx leads to early lethality in mouse embryos around E6.5. Moreover, sequencing studies have revealed the importance of DAXX in human tumors. Mutually exclusive mutations in DAXX and ATRX occur in about 30% of pancreatic neuroendocrine tumors (PanNETs). Although lots of progress …
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The Contribution of Retrotransposons to the Transcriptomes of Murine Somatic Cells
… comprise approximately 40% of the mouse genome. Once thought to be useless “junk” DNA, there is growing evidence that retrotransposons play crucial roles in genome evolution and gene regulation, and contribute to the transcriptome. Several studies have found functional …