Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 87 for “"Mouse Embryos"”.
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ULTRARAPID FREEZING OF MOUSE EMBRYOS
… involves a brief exposure (2.5-3 minutes) of embryos to a high concentration (3.0-4.5 M) of dimethyl sulfoxide (DMSO) and 0.25 M of sucrose at room temperature before direct plunging into liquid nitrogen. The objective of this study was to determine the efficacy of this technique for the …
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Storage of mouse embryos of different genotypes
… to the difference in the percentage survival of embryos after cooling and storage. A total of 2669 eight-cell mouse embryos of different genotypes (ICR, C57BL/6n, ICR x C57 and C57 x ICR) were stored for one day at 4$\sp{\underline\circ}$C or at $-$196$\sp{\underline\circ}$C after vitrification …
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Development of the ocular motor system in mouse embryos
… oculomotor guidance in chick and zebrafish embryos; however, a time course of mammalian development has not been established. This study establishes a time course of the critical stages of oculomotor nerve outgrowth to the periocular area in mice at embryonic day (e) 9.5 - e14.5. In previous …
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Analysis of 3D Genome Organization in 4-Cell Mouse Embryos
… we studied the 3D genome organization in early mouse embryos at the 4-cell state based on in-situ sequencing data. Interestingly, while at this stage the cells have only undergone 2 divisions, researchers have found that the type of division (equatorial or meridional) matters for the chance of …
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Rapid Functional Dissection of Genetic Networks Via RNAi In Mouse Embryos
… particles carrying shRNA or Cre-recombinase into mouse embryos, I demonstrated a highly efficient, non-invasive, selective transduction of surface epithelium. Epidermal progenitors stably incorporated and propagated the desired genetic alterations. Importantly, I achieved epidermal-specific …
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Development of Mouse Embryos in Vitro and in Vivo, Following Mechanical Division
Mouse embryos were divided in halves and in quarters to determine the capability of fractional embryos to develop into live fetuses. When embryos were divided in half at the 2-cell, 4-cell, 8-cell and morula stage, a higher percentage of blastocysts developed in vitro from half 8-cell embryos …
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Effects of iloprost on embryo development in vitro
Apoptosis (programmed cell death) can occur in embryos at any point during development. Apoptosis can occur naturally in embryo development due to a myriad of reasons including suboptimal culture conditions. It has recently been reported that Iloprost, a biosynthetic prostacyclin analogue and …
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Phage Display to Identify Peptides Binding to or Penetrating the Mouse Zona Pellucida
… phage display techniques, which bind sites on mouse embryos, ovaries, cytoplasmic membranes and/or intracytoplasmic components. Specifically, M13 coliphage 7-mer, 12-mer and 15-mer random peptide libraries were used separately for biopanning. Peptides derived from the amplified pools were …
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Maternal Effects on Embryonic and Placental Growth and the Penetrance of the Luxate Gene in Mice.
… before hind-limb bud formation by irradiation of mouse embryos at 8 and 9 days of development (Russell, 1950). Russell's observations are significant because they suggest that polydactyly results from more than minor local disturbances of footplate morphogenesis.
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An in vitro model of mouse corneal endothelial development Mohammed Mubeen Goolam.
… a mesenchymal-to-epithelial transition (MET) in mouse periocular mesenchyme cells to drive them to form fully differentiated adult corneal endothelial cells in culture. Periocular mesenchyme surrounding the optic cup was dissected from E12.5 and E13.5 mouse embryos and immortalised with the SV40 …
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Utilising embryonic and extra-embryonic stem cells to model early mammalian embryogenesis in vitro
… the body. After implanting into the uterus, the mouse embryo is comprised of three cell lineages: first, the embryonic epiblast (EPI) that forms the embryo proper, second, the extra-embryonic ectoderm (ExE) which contributes to the foetal portion of the placenta, and third, the visceral endoderm …
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Charakterisierung der Selenoproteine Thioredoxinreduktase 1 und 2 anhand von Knock-out-Mausmodellen
… the establishment of a conditional TR1-knockout mouse strain and the phenotypical characterization of TR1- and of TR2-knockout mice, that have been established before. Both knockouts, TR1 and TR2, showed to be embryonically lethal. TR1 deficient mouse embryos died at day 9.5, whereas TR2 …
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Development of mouse morulae after encapsulation in alginate microgels or poly-l-lysine microcapsule
… a-intact (ZPI) and zona pellucida-free (ZPF) mouse embryos after encapsulation in either 2% sodium alginate or 0.1% poly-L-Iysine (PLL). In Experiment 1, rate of development of ZPI embryos (n = 150) from morulae to hatched blastocysts was measured after encapsulation in alginate or PLL and as …
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The Roles of Krüppel-Like Factor 1 (KLF1) in the Human Fetal Erythroid Compartment.
… and adult erythropoiesis. KLF1 knockout mouse embryos die due to severe anemia. Dominant human mutations in KLF1 can cause hereditary persistence of fetal hemoglobin. We show that KLF1 positively regulates β-globin and Bcl11A gene expression using KLF1 knockdown in in vitro-differentiated …
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Behavioral Changes in Adult C57BL/6J Mice following Prenatal Exposure to Ethanol.
… a model for binge drinking, in which C57BL mouse embryos were exposed to alcohol during 2 separate critical periods of brain development, gestational day (GD) 7 or 8. As adults, the offspring were tested to determine if loco-motor activity and emotional reaction to a novel environment had …
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Alternatively spliced isoforms of Fibronectin, Tenascin-R and other potential players in early vasculogenesis
… observed in the yolk sacs of EIIIA/B double-null embryos by as early as embryonic day E9.5, and these defects are likely due to alterations in the extracellular matrix (ECM). Therefore, I have conducted this study to investigate the differences in the ECM composition of the yolk sac in the …
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The feasibility of interspecific rescue of endothelial/hematopoietic lineage deficiency
Here we propose to develop a mouse model which will establish “proof of principle” for future development of large animal (e.g. pig) models which could be used as actual human solid organ and blood donors as well as in pre-clinic trials. As a proof of concept, we intended to develop interspecific …
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