Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 60 for “"Motor protein"”.
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Theoretical aspects of motor protein induced filament depolymerisation
… in cells are driven by highly specialised motor proteins, which interact with the cytoskeleton: a network of filamentous structures, e.~g.~ actin filaments and microtubules, which organises intracellular transport and largely determines the cell shape. These motor proteins are able to …
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Single-molecule studies of unconventional motor protein myosin VI
… members that are actin-based molecular motors. It has received special attention due to its distinct features as compared to other myosins, such as its opposite directionality and a much larger step size than expected given the length of its “leg”. This dissertation presents the author‟s …
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Site-directed mutagenesis of the ncd microtubule motor protein
Ncd is a member of the kinesin family of motor proteins. Ncd is involved in the processes of meiosis and early mitosis in <i>D. melanogaster</i>. PCR-mediated site-directed mutagenesis was utilized to introduce specific mutations into pET/MC6, a construct containing the motor domain of ncd. Six …
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Effects of Cysteine Modification on Microtubule-Motor Protein Function and Tubulin Assembly
… been shown to inhibit the activity of MT-motor proteins and tubulin assembly. This project seeks to investigate the mechanisms by which NEM affects motor function and inhibits MT minus end assembly. Recombinant motor domains of Drosophila kinesin (DK350 and DK375), Ncd (MC1), and squid …
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Leveraging the motor protein Kinesin to manipulate DNA molecules in synthetic environment
… der NanoBioTechnologie. Ihr Ziel ist es, das Motorprotein Kinesin und Mikrotubuli einzusetzen, um DNS-Moleküle in einem synthetischen Umgebung zu manipulieren. Diese Doktorarbeit setzt sich aus fünf Kapiteln zusammen. In der Einführung wird die makromolekulare Struktur der Zelle beschrieben, …
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Adaptive Control of an Optical Trap for Single Molecule and Motor Protein Research
… of the mechanics of single molecules and motor proteins. This instrument provides a more precise means of manipulating biological specimen as well as a tool for nanofabrication and has the potential to expand the knowledge base of DNA, chromosomes, biomotors, motor proteins, reversible …
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Characterizing the cargo binding and regulatory function of the tail domain in Ncd motor protein
… disjunctional (Ncd) is a kinesin-14 microtubule motor protein involved in the assembly and stability of meiotic and mitotic spindles in Drosophila oocytes and early embryos, respectively. Ncd functions by cross-linking microtubules through the tail and motor domains. It was originally believed …
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Structural Characterization of the C-terminus of the Cochlear Motor Protein Prestin: Intrinsic Disorder Linked to Voltage Sensor Function
… properties that support this function. The motor protein called prestin resides in the lateral wall of the OHCs. Prestin undergoes voltage-dependent conformational changes, which correlates to the movement of charge through the membrane or non-linear capacitance (NLC). This property …
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Learning the ABC's of Ribose Transport Using Biophysical Methods
… transporters comprise a large superfamily of proteins that are involved in a variety of biological phenomenon, from bacterial metabolism to cellular homeostasis, antigen-presentation, and drug resistance. These proteins are implicated in a variety of clinically relevant phenomenon, including …
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Approaches to Study Small Molecule Inhibitors and Their Targets
… inhibitor analogs is crosslinked to the target protein to yield a robust signature for identifying inhibitor-modified peptide fragments in complex mass spectrometry data. I applied this approach to an ATP-competitive inhibitor of kinesin-5, a widely conserved motor protein required for cell …
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Relationship between Hepatitis B Virus Core Particles and Intracellular Motility
… 30 min, suggesting that the minus end-directed motor protein, dynein, might be actively involved. ER and peroxisome are aggregated to perinuclear region like mitochondria in HBV replicating stable cells. Also, HBV core particles and MTs were colocalized in HBV replicating cells, indicating that …
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KINESIN-MICROTUBULE INTERACTIONS DURING GLIDING ASSAYS UNDER MAGNETIC FORCE
Conventional kinesin is a motor protein capable of converting the chemical energy of ATP into mechanical work. In the cell, this is used to actively transport vesicles through the intracellular matrix. The relationship between the velocity of a single kinesin, as it works against an increasing …
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Viral and cellular proteins involved in vaccinia virus egress
… to facilitate efficient viral spread. The viral proteins A36, F12 and E2 together with the kinesin-1 microtubule motor protein are thought to be involved in IEV egress from the site of wrapping to the cell periphery, although the exact mechanism of movement is unclear. Until recently, A36 was the …
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Building KiMoSin : design requirements for kinetic interfaces in protein education
Design guidelines for tools to enhance protein education are developed and applied to a prototype tool. A literature search and personal experience suggest kinetic, tangible models fill the current gaps in protein education. Thirty-six personal interviews with biology instructors and students set a …
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A feedback analysis of outer hair cell dynamics
… potentials which results in contraction. The motor protein prestin is driven by receptor potentials. However, low-pass filtering by the plasma membrane should severely attenuate the receptor potential at high frequencies (> 100 kHz) where mammalian hearing has been observed. Thus, an open …
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A Role for Dynamin-Dependent Endocytosis During Drosophila Gastrulation
… relationship between Dynamin and the Myosin II motor protein. Lastly, localization and functional studies I performed for this work suggest a role for Sorting Nexin proteins during plasma membrane reorganization required for Dynamin-dependent endocytosis.</p>
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Experimental and theoretical investigation of mechanism of Kinesin motility
Kinesin is a motor protein capable of utilizing chemical energy from ATP hydrolysis to generate mechanical force to power its progressive motility along a microtubule track. The mechanism of motility has been a subject of extensive study for last decade. Recently, it has been proposed that novel …
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Synaptic plasticity in the MyosinVa mutant mouse
The trafficking of essential proteins into spines is an important aspect of synaptic plasticity. MyosinVa, an actin-based motor protein, has been implicated in the synaptic delivery of AMPARs during LTP [1]. However an earlier study showed that LTP and LTD were unaffected in the MyosinVa-null …
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The encapsidation protein from Lactococcus lactis asccφ28 as a novel system for the study of molecular motors
Motor proteins, molecules capable of converting the cell’s free energy into mechanical work, are prevalent in life. However, despite their pervasiveness, our understanding of these molecular machines remains inadequate. Some of the simplest examples of molecular motors are found in many …
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Genetic analysis of the maintenance of neuronal morphology in Drosophila melanogaster
… activates the minus-end-directed microtubule motor protein Dynein, causes mislocalization of photoreceptor nuclei basally, even into the optic stalk and brain. Photoreceptors in animals mutant for the Dynactin subunit Glued retain apical markers, but have a bipolar-like morphology with the …
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