Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Motile cells"”.
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Actin remodeling in motile cells
… and distributions of cytoplasmic actin. In cells, actin cycles between monomeric and polymeric phases tightly regulated by actin binding proteins that control cellular architecture and movement. Here, we characterize actin remodeling in shear stress stimulated endothelial cells and in actin …
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Probing the regulatory mechanisms of the actomyosin system in motile cells
… was to study the regulatory mechanisms of the motile machinery by selecting a representative key player for each stage of the signaling process: the regulation of Arp2/3 activity by WASP (actin system), the role of cGMP in myosin II assembly (myosin system), and the influence of …
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Motility and Chemotaxis of Erwinia Herbicola and Its Effect on Erwinia Amylovora (Biological Control, Phytobacteriology, Fire Blight)
… were determined by the capillary assay. Actively motile cells were selected from the periphery of swarming colonies grown on soft agar (0.25%) modified Emerson's medium plates minus the ingredient nutrient broth. The optimum growth temperature for producing motile cells was 29 C. Cells were still …
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Chemotaxis Methyltransferase Ii From Bacillus Subtilis
Chemotaxis is the process by which motile cells sense and respond to their chemical environment. Following a period of time during which chemotactic bacteria respond to chemical stimulation, they revert to their pre-stimulus behavior, or adapt. Biochemically, adaptation is correlated with the …
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Phytoplankton in flow
… phytoplankton species found in these layers are motile. We demonstrate that layers can form when the vertical migration of phytoplankton is disrupted by hydrodynamic shear. Using a combination of experiments, individual-based simulations, and continuum modeling, we show that this mechanism - …
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Deformation mapping of fibronectin fibrils using digital image correlation
Bone cells produce large amounts of extracellular matrix (ECM) proteins that form a fibrillar scaffold on which bone mineral is deposited. Prior work has shown that fibronectin fibrils are one of the earliest ECM proteins to be assembled. Time lapse imaging techniques show that the deposition of …
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Cytometry of Reaction Rate Constant (Crrc): Optimization of the Technique and Development of a Novel Protocol to Assess Cell Population Heterogeneity Based on Aldh1a1 Activity in Vitro
… However, CRRC showed poor robustness to highly motile cells. Here, I report on the development of a workflow to make CRRC robust to cell motility. The novel workflow was used to develop a protocol for in vitro CRRC studies of aldehyde dehydrogenase 1A1 (ALDH1A1)-based cell heterogeneity. The …
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New methods for cryopreserving rooster spermatozoa
… is a common cryoprotectant used to protect cells during freezing, but its relatively large molecular weight and relatively low membrane permeability (compared to water) creates relatively long aniosmotic conditions when glycerol is added or removed. Glycerol-exposed rooster spermatozoa …
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Chemotaxis of Central Nervous System-derived Tumor Cells within Controlled Microenvironments
… Despite the well-documented migration of MB cells to other parts of the brain and spinal column, MB chemotaxis is poorly understood. Herein, we examined the in vitro migratory and cellular responses of MB-derived cells to external signaling of Epidermal Growth Factor (EGF), hepatocyte growth …
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Switching in bacterial gene expression networks
… as a population instead of individual cells. By allowing individual cells to stochastically switch between multiple phenotypes, the cell population can make sure some cells are always fit for the environmental change. The underlying genetic circuitry plays a key role in eliciting …
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HDAC6 activity is required for efficient polarization and intracellular transport of organelles in directionally migrating cells
… role in polarization of directionally migrating cells, we hypothesized that HDAC6 activity regulates important MT dependent aspects of directional cell motility. We were able to test this hypothesis by observing cellular polarization and organelle and vesicle transport in the presence or absence …
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Transport in Biology, from Theory to Application
… on chemotaxis. Chemotaxis is a response of motile cells or organisms in which the direction of movement is affected by the gradient of a diffusible substance. In multicellular organisms, chemotaxis is critical to development as well as normal function. We also include a specific application …
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The Function of CARMIL1 in Migrating Cells
… Capping Protein- CARMIL interaction in migrating cells. I establish the CARMIL-CP complex as a key regulator of lamellipodial actin assembly and of lamellipodial dynamics. Membrane ruffling at the leading edge of motile cells and macropinocytosis were also found to be dependent on the CARMIL1-CP …
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Isolation and characterization of motility-defective mutants of Haloferax volcanii.
Haloferax volcanii is a motile extreme halophile, and a detailed physical map of its genome exists. An effort is underway to locate more genes and to increase the detail of this map. Flagellated motile bacteria possess a number of filaments that function as cellular propellers. Motile bacteria swim …
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Oxygen Sensing by the Aer and Tsr Receptors of Escherichia Coli
… the two was not possible, previously, in live, motile cells. I differentiated PMF from electron flow by creating a series of ETS mutant strains that express ternary combinations of i) aerotaxis receptors, ii) NADH dehydrogenases and iii) cytochrome oxidases. Since the ETS components differ in …
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High-Throughput Single-Cell Functional and Molecular Profiling of Immune Cells in Cancer Immunotherapy
… the adoptive transfer of genetically modified T cells reprogrammed to target tumor antigens have shown remarkable responses. Despite their promise, the efficacy of adoptive immunotherapy remains unpredictable due to the heterogeneous nature of the infusion products, patients’ characteristics, …
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Investigating the effect of phosphodiesterase inhibitors on human sperm motility and function
… This is done using CASA throughout to record motile and progressively motile cells. Experiments were carried out on various PDE inhibitors, some of which have previously been reported as having an effect on sperm motility and some which have never been investigated before. The study then goes …
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Protein Phosphatase 4 in human spermatozoa: investigations into location, regulatory subunits and role in motility
… increases in the percentage of progressively motile cells, ALH, BCF, STR and LIN were observed. The results presented here provide evidence for the first time of the presence of the catalytic subunit of PPP4 in human sperm and insights into its associated proteins. Evidence is also provided …
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Investigation of Neutrophil-Like HL-60 Cell Migration in a 3D Collagen Matrix
… role in human health. Among the immune cells, neutrophils are one of the most essential cells in protecting the body against invading pathogens. In the presence of chemoattractants, these cells are the first responders that start a directed migration toward the injured area. As …
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Regulation of integrin-mediated linkages during cell migration
… from the cell membrane. At high adhesiveness cellsubstratum detachment is the rate-limiting step in cell migration speed while at low adhesiveness other factors, such as the formation of new adhesions, are likely to limit cell speed. We identified calpain, a calcium-dependent protease, as a …
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