Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 116 for “"Molecular methods"”.
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Innovative molecular methods for fish species differentiation
This thesis presents the results of a proof-of-principle study in which a rapid screening method for various market-relevant fish species have been developed. A DNA microarray was established using a set of 100 universal oligonucleotide probes. DNA probe signal patterns were observed to be …
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Authentication of Species in Bison Products Using Molecular Methods
<p>American bison (<em>Bison bison</em>) meat is vulnerable to species substitution due to its high value and similar appearance to less expensive meats, such as beef from domestic cattle (<em>Bos taurus</em>). DNA barcoding of the cytochrome <em>c </em>oxidase subunit 1 (CO1) gene is a commonly …
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Investigation of bacteria associated with Australian wine grapes using cultural and molecular methods
… species using a combination of cultural and molecular methods. Red (Shiraz, Cabernet Sauvignon, Merlot) and white (Chardonnay, Semillon, Sauvignon Blanc) grape varieties were examined. Factors affecting the bacterial ecology of grapes were considered. The bacterial populations of mature …
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Investigation into the use of molecular methods to distinguish between species of Caladenia subgenus Calonema
The phylogeny of typical spider orchid (Caladenia subgenus Calonema) is investigated for the first time. The analyses were performed using 17 RAPD and 10 ISSR primers on 30 taxa representing the three spider orchid groups (the dilatata, patersonii and reticulata groups) yielding 135 RAPD and 63 …
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Use of Molecular Methods to Authenticate Animal Species and Tissue in Bovine Liver Dietary Supplements
… to fraud due to the lack of modern detection methods available for processed animal tissues and premium pricing for dietary supplements. Therefore, the objective of this research was to use molecular methods to authenticate dietary supplements claiming to contain “bovine liver” or “beef liver” …
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Applying molecular methods to investigate potential anthropogenic impacts on ageing in a free-living bat species
… sensitive to disturbance, subtle changes at the molecular and microbial level may provide an early indication of population-level impacts. This thesis examines whether diAerences in ageing and gut microbiome between two colonies of Bechstein’s bat (Myotis bechsteinii), a long-lived woodland …
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Geomicrobiology of nitrogen in a coastal aquifer : isotopic and molecular methods to examine nitrification and denitrification in groundwater
… groundwater systems. Here we use isotopic and molecular biological methods to identify the populations of nitrifiers and denitrifiers, where they occur, and what levels of activity are present through the upper four meters of a coastal groundwater system. This work shows two different …
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Molecular methods for the detection of TEM- and SHV-related beta lactamase genes in members of the Enterobacteriaceae
… primary aim of this study was to evaluate two molecular techniques for the detection of SHY and TEM-related genes in clinical isolates. The study sample consisted of 209 clinical isolates of enteric Gram negative bacilli, isolated at Groote Schuur Hospital microbiology laboratory. The isolates …
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Geomicrobiology of nitrogen in a coastal aquifer : isotopic and molecular methods to examine nitrification and denitrification in groundwater
… groundwater systems. Here we use isotopic and molecular biological methods to identify the populations of nitrifiers and denitrifiers, where they occur, and what levels of activity are present through the upper four meters of a coastal groundwater system. This work shows two different …
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Detection and characterisation of enteroviruses and the gut virome in islet autoimmunity and type 1 diabetes using novel molecular methods
… as a prominent candidate factor. Advances in molecular techniques including next generation sequencing (NGS) have enabled sensitive and high-throughput virus detection, improving on cell culture and serology techniques. There are over 110 human/primate EVs identified, classified within four …
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Detection of the calreticulin type 1 and type 2 mutations in a group of myeloproliferative neoplasm patients using molecular methods
The myeloproliferative neoplasms (MPN), formerly referred to as chronic myeloproliferative disorders, are a group of haematopoietic stem cell disorders that are characterized by clonal proliferation of one or more mature myeloid lineages. The group of disorders, now classified as the classic MPNs, …
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Aster Yellows Phytoplasma, Aster Leafhopper And Canola: Development And Application Of Improved Molecular Methods For Pathogen Detection And Genetic Characterization Provide Increased Understanding Of Aster Yellows Disease
… most severely. Even though there are several methods to control AYp spread, the most common method is insecticide spray, which can damage the environment if used unsustainably. Tools that can rapidly diagnose phytoplasma infection and accurately identify phytoplasma strains are of critical …
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Characterization of Ulva (Ulvaceae, Chlorophyta) species cultured in commercial abalone farms in South Africa, and comparison with closely related wild species, using morpho-anatomical and molecular methods
… were identified using morpho-anatomical and molecular methods. The molecular markers employed in this study were the plastid large subunit of Ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL), Internal Transcribed Spacer of nuclear ribosomal DNA (ITS nrDNA) and the elongation factor …
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Survival and recovery characteristics of Arcobacter butzleri in groundwater microcosms
… environmental sources comparing cultural and molecular methods. Cultural methods using selective media proved inferior to molecular methods. Molecular detection also had limitations, though. Selective enrichment and nucleic acid purification may resolve these limitations.
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The effects of age and size of canopy gaps on the mycorrhizae of yellow birch (Betula alleghaniensis Britton) and sugar maple (Acer sacchrum Marsh.) from deciduous forests of the Québec city region
… and characterized using morphotyping and molecular techniques. In the 1-yr old 1000 m 2 cuts, colonization increased in the maple roots as the season progressed. In October, there were more arbuscules in the seedlings from the gaps vs those from the uncut areas. No differences in ECM …
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Žarnyno Sarcocystis infekcijos paplitimas rudosiose lapėse (Vulpes vulpes) /
… method for the infections level determination. Molecular methods such as DNA extraction, PCR and electrophoresis helped to determine the species and their distribution. By the microscopic analysis seventeen of twenty foxes had sarcocysts. The distribution of this genus is 85%. Molecular methods …
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Detectability, movement, and population structure of the endangered Candy Darter in Virginia
… riffle species. Using physical marking and molecular methods, I describe movement patterns and provide insight into the spatial distribution of related pairs and reproductive contribution of the endangered Candy Darter. Minimum detected movements of adults were greater for molecular methods …
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Methods to interrogate cells and their interactions with single-cell resolution
Only recently have molecular methods achieved high-quality and unbiased representations of diverse intracellular molecules at the single-cell level. With this technological advancement, researchers have begun deconvolving population-level measurements to understand whether prior observations were …
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