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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 158 for “"Molecular cloning."”.
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Tobacco Phospholipase D β1: Molecular Cloning and Biochemical Characterization
Transgenic tobacco plants were developed containing a partial PLD clone in antisense orientation. The PLD isoform targeted by the insertion was identified. A PLD clone was isolated from a cDNA library using the partial PLD as a probe: Nt10B1 shares 92% identity with PLDβ1 from tomato but lacks the …
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Molecular Cloning of the cDNA for Porcine Parotid Hormone
<p>It has been found that parotid glands secrete a factor into blood that stimulates dentinal fluid movement, which in turn, prevents the development of dental caries. This factor, designated as parotid hormone, has been isolated from porcine parotid glands and its partial amino acid sequence has …
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Molecular cloning and characterization of two Arabidopsis thaliana sulfotransferases
… and secondary alcohols with unknown kinetics. Molecular characterization of AtST4a and AtST6 revealed that the deduced protein sequence of both enzymes contain all the amino acids involved in PAPS binding and catalysis (Marsolais et al. 1995). Expression studies failed to determine conditions …
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Molecular cloning and analysis of the genome of bovine parvovirus
… can occur during replication of parvovirus DNA. Cloning of BPV terminal sequences permitted the identification of the 3’ flop sequence inversion as a natural component of BPV DNA. This is the first report of sequence inversions within the 3’ end of an autonomous parvovirus. Clones with the 3’ …
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Molecular cloning and characterization of 3-methylcrotonyl-CoA carboxylase from soybean
… subunit and a 59 kDa biotin-free subunit, with a molecular weight of approximately 970,000 ± 83,000. Soybean MCCase appears to have an [alpha][subscript]6[beta][subscript]6 quaternary structure;Initial velocity kinetic studies of soybean MCCase with competitive inhibitors demonstrated that soybean …
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Molecular Cloning and Structure-Function Analysis of the Plant Proton-Sucrose Symporter
"To explore other functionally-important domains, the AtSUC1 cDNA was randomly mutagenized and transformed yeast were screened on sucrose- or maltose-limited media for growth. Three point mutations were identified---H65Q, G334S, and L461P. Significantly, they all displayed enhanced sucrose …
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Molecular Cloning and Expression of the Pyrimidine B Gene From Bacillus Subtilis
The aspartate transcarbamylase of Bacillus subtilis is an example of a "vegetative" gene product which ceases to be synthesized once the cells begin to sporulate. To study the regulation of expression of this enzyme, the gene which encodes it (pyrB) was cloned from B. subtilis. Chromosomal DNA from …
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Molecular Cloning of Avian Sarcoma Virus CT10 and Characterization of its Protein Product
… oncogenes. Biologically active CT10 DNA was molecularly cloned and the nucleotide sequence was determined. The CT10 genome encodes a gag-fusion polypeptide of 47 kilodaltons, termed p47<sup>gag-crk</sup>. This protein contains blocks of sequence similarity to a noncatalytic, potentially …
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Molecular cloning, structural characterisation and functional study of peptides from amphibian skin secretion
The skin secretions of amphibians contain large amounts of biologically-active compounds, such as antimicrobial peptides (AMPs), anticancer peptides and trypsin inhibitors. Several kinds of AMPs, isolated and characterised from the skin of amphibians, have been studied by researchers to provide …
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Molecular cloning of three genes encoding SNF1/AMPK protein kinases from Drosophila melanogaster.
… in a number of different organisms and recent molecular and biochemical evidence has revealed that the mammalian AMP-activated protein kinases (AMPKs), known to regulate fatty acid and sterol metabolism in response to stress, represent the mammalian homologues of SNF1. Three novel Drosophila …
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Molecular cloning, structural characterisation and functional study of bioactive peptides from amphibian skin secretion
… of electrical stimulation. Afterwards, ‘shotgun’ cloning was applied using 3’RACE PCR to obtain full length nucleotide sequence that encoded the open reading frames of peptide biosynthetic precursors. Three putative peptides were identified by cloning (Kunitzin-AH, QUB-1617, QUB-1629), and each …
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Isolation, reconstitution, and molecular cloning of the manganese-containing superoxide dismutase from Deinococcus radiodurans
… activity of 3300 units/mg and an apparent molecular mass of 43,000 daltons. The enzyme contains 1.5 gram-atom of manganese per mol dimer, and is composed of two identical subunits of 23,500 daltons. The enzyme rapidly loses its catalytic activity and metal content upon dialysis in …
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Identification, molecular cloning and modification of skin-derived bioactive peptides from selected Hylidae and Ranidae
… baltea and Hylarana guentheri, through ‘shotgun’ cloning. In parallel to this strategy, predicted mature peptides were identified and isolated from skin secretion using HPLC and structures verified by LC-MS. The peptides were then chemically-synthesised to produce the quantities required for …
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Identification, Molecular Cloning and Functional Characterisation of Novel Bioactive Peptide Drugs from Amphibian Skin Secretions
… in liquid nitrogen. Afterwards, a series of molecular techniques, including mRNA isolation, cDNA library construction, PCR, agarose gel analysis and DNA sequencing were applied. The novel peptide sequences were deduced from cloned cDNAs and then confirmed by using MALDI-TOF mass spectrometry …
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