Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 88 for “"Molecular characterisation"”.
-
Biochemical and Molecular Characterisation of the FADDosome
An anti-metabolite drug, 5-FU (5-fluorouracil), is widely used in the treatment of colorectal cancer. However, acquisition of resistance by cancer cells is the main cause of the limited clinical use of 5-FU, which needs to be overcome to widen and lengthen its utility. Therefore, it is necessary to …
-
Molecular characterisation and functional properties of gum arabic
… chromatography (gpc) was used to isolate several molecular mass components of gum arabic. The u. v. (218 nm) gpc elution profile showed the presence of several prominent peaks; however, only one major peak (FAw 3.8 x 10-9 g moll) and a minor peak (FAw 1.45 x 106 g mol-1) were observed in …
-
Expression, Purification and Molecular Characterisation of Sideroflexin Proteins
… The work in this thesis aims to describe the molecular properties of the sideroflexin proteins in order to elucidate their function. In the first part, yeast and the five human sideroflexin proteins were successfully expressed in mitochondria of Saccharomyces cerevisiae and purified to …
-
Molecular characterisation of erythropoietic protoporphyria in South Africa
Includes bibliographical references.
-
Molecular characterisation of acute intermittent porphyria in South Africa
… will facilitate an understanding of the molecular basis of AIP in South Africa, and provide a platform for the screening of family members of affected patients. Identification of latent carriers would allow for education with respect to precipitants and how best to avoid them, so as to …
-
The agronomic and molecular characterisation of Rht8 in hexaploid wheat
… emerging Triticeae genomic resources to identify molecular markers. Rht8 was reduced to a 1.015 cM genetic interval and syntenic intervals of 1.34 Mb on rice chromosome 4, 1.36 Mb on Brachypodium chromosome 5, 2.9 Mb on barley 2H and 4.25 Mb on Ae. tauschii 2D. Disruption to micro-collinearity was …
-
Molecular Characterisation of Exoplanetary Atmospheres using High-Resolution Transmission Spectroscopy
… spectroscopy into the next era of atmospheric characterisation, that of low-mass planets. In this thesis, we demonstrate that it is feasible to characterise the atmospheres of small and temperate exoplanets using ground-based high-resolution transmission spectroscopy. We investigate the …
-
The biochemical and molecular characterisation of respiratory mucins in TB
… respiratory mucins and TB at a biochemical and molecular level.
-
The molecular characterisation of pregnancy-associated plasma protein-A (PAPP-A).
… pregnancy. In this thesis a biochemical and molecular characterisation of PAPP-A was performed. The polyclonal antiserum (DAKO) directed against PAPP-A has been shown to also interact with proteins other than PAPP-A. These non-specific interactions were abolished by performing Western …
-
Molecular characterisation of the <i>Rhynchosporium commune</i> interaction with barley
The molecular interaction between Rhynchosporium commune and its host barley was studied to gain a better understanding of the pathogen during infection and provide further characterisation of resistance in barley, using a combination of bioinformatics, transcript expression analysis, proteomics …
-
Biochemical and molecular characterisation of cell wall glycosyltransferases in Mycobacterium tuberculosis
The human pathogen and aetiological agent of tuberculosis, Mycobacterium tuberculosis has a cell wall architecture similar to the non-pathogenic bacterium Corynebacterium glutamicum. The availability of their genome sequences has enabled the utilisation of C. glutamicum as a model for the …
-
Molecular Characterisation and Therapeutic Targeting of NPM1-mutant Acute Myeloid Leukaemia
… (AML) cases. Despite their prevalence, the molecular consequences of NPM1 mutations (NPM1c) are incompletely understood and this hinders progress in designing effective therapeutic strategies. This thesis describes my work to characterise NPM1-mutant cells at the molecular level using …
-
Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes
The phytohormone ethylene is essential to many plant developmental processes, of which the control of climacteric fruit ripening is among the best characterised. However this hormone eventually causes fruit rotting which results in a non-marketable product. One approach to reduce ethylene synthesis …
-
Molecular Characterisation of Diffuse Large B-cell Lymphoma in South Africa
… least 5 new groups, each with distinct genetic, molecular and clinicopathologic features, revealing potentially novel drivers of the disease. There is therefore a need to further understand the molecular pathology of these distinct subtypes, including within the context of HIV. The latter formed …
-
Molecular characterisation of the role of systemic infections in Alzheimer’s disease brain
… investigates how systemic infection reshapes the molecular architecture of the AD brain and introduces Genedex, a framework to contextualise transcriptional findings within the broader AD continuum. Using post-mortem prefrontal cortex samples from the UK Brain Bank Network, bulk RNA sequencing …
-
Pharmacological and Molecular Characterisation of P2Y Receptors in Endothelial and Epithelial Cells
… in order to provide a rapid screen for the molecular determinants of these receptors in these cells. These studies also aimed to confirm previously published pharmacology, thus, to set the basis for western blot studies using P2Y receptor antibodies. Bovine aortic endothelial cells that …
-
Functional and molecular characterisation of two stomatin-like proteins from Arabidopsis thaliana
… The aim of this thesis was to provide a detailed characterisation of these two genes on a molecular and functional level. Both proteins are expressed ubiquitously throughout plant development, but they accumulate at particularly high levels in pollen and other metabolically active cells. …
Page 1 of 5