Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 517 for “"Molecular and Cell Biology"”.
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Quantitative analysis and modulation of the spatial operation of the EGF receptor autocrine cell signaling system
… is a critical factor for tissue development and homeostasis. It is difficult to analyze these systems, however, because they are both self-contained and recursive. To understand how cellular parameters influence autocrine activity, we investigated a human epidermal growth factor/epidermal …
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Impedance spectroscopy for cellular and biomolecular analysis
The application of microfabrication technology to molecular and cell biology has motivated the rapid development of a novel class of microdevices collectively known as ‘Lab On a Chip’ devices.<br/>Impedance spectroscopy is a non-invasive, label-free, analytical technique that is amenable to …
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Measurement of rapid protein diffusion in the cytoplasm by photoconverted intensity profile expansion
… function. To measure protein diffusion in cells, researchers have developed multiple fluorescence microscopy methods, and have tested them rigorously. However, using these methods for precise measurement of diffusion coefficients requires expertise that can be a barrier to broad utilization …
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Investigating pseudohypoxia in renal cells deficient in fumarate hydratase.
… hydratase (FH), is found mutated in hereditary and leiomyomatosis and renal cell cancer syndrome, whereby affected cells are characterized by high subcellular accumulation of the metabolite fumarate. Fumarate has been shown to inhibit the prolyl hydroxylase-dependent degradation of hypoxia …
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Desiccation-driven senescence in the resurrection plant Xerophyta schlechteri (Baker) N.L. Menezes
… process that involves the degradation of cellular metabolites and photosynthetic pigments and uncontrolled dismantling of cellular membranes and organelles. Angiosperm resurrection plants display vegetative desiccation tolerance and avoid drought-induced senescence in most of their …
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The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants
The strategy of "complementation by functional sufficiency" was used to isolate a cDNA, designated XVSAP1, from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern …
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The functional characterisation of the XhABFA transcription factor from the resurrection plant Xerophyta humilis
… the ability to survive loss of up to 95% of cellular water in leaves and roots, is rare amongst vascular plants. However, the trait has evolved multiple times in a small, diverse group of angiosperms collectively known as “resurrection plants”. The physiological, morphological and metabolic …
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Eragrostis nindensis: unravelling senescence in an African desiccation tolerant grass
… called “resurrection plants”, that can withstand up to 95% tissue water-loss without compromising viability by, inter alia, undergoing extensive metabolic reprogramming and suppressing senescence. In this thesis the African desiccation tolerant grass Eragrostis nindensis (Fical & Hiern) was …
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In vitro tissue culture: Towards conservation of threatened desiccation sensitive Encephalartos cycads seeds
… Recalcitrant seeds have a short lifespan and cannot be dried or stored for prolonged periods as they become non-viable when they lose moisture. While studies on cryopreservation for conserving cycad germplasm and banking these desiccation-sensitive seeds has made some advances, cycad …
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Probiotics for the management of kidney stone disease
… humans is either absorbed into the urinary tract and excreted in urine or degraded by gut bacteria. Oxalate-degrading gut bacteria play a critical role in human oxalate homeostasis, evidenced by increased research interest in them as potential probiotics in the management of kidney stone disease. …
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The Development of an African Horse Sickness Virus VP7 Quasi-Crystal Vaccine Candidate in N. benthamiana
… a debilitating viral disease affecting equines and has resulted in many disastrous epizootics. To date, no successful therapeutic treatment exists for AHS and the commercially used live-attenuated vaccines (LAVs) have various side effects. Insoluble particulates have been shown to increase …
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Development of West Nile virus candidate vaccines in Nicotiana benthamiana
… America, Europe, the Middle East, West Asia and Australia. The virus is vectored by Culex mosquitoes and is maintained in a bird-mosquito transmission cycle with hundreds of bird species acting as reservoir hosts. In humans, infections can develop into febrile illness and severe …
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Biomolecular condensation of intrinsically disordered regions in P. falciparum and H. sapiens transcription factors
… falciparum is still a major cause of disease and death, especially in sub-Saharan Africa. The organism's complex life cycle is tightly coupled to a carefully controlled gene expression programme, and its highly divergent transcriptional machinery suggests unique mechanisms of transcriptional …
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Evaluation of three heterologous xylose isomerase genes for the fermentation of plant biomass to bioethanol in Saccharomyces cerevisiae
… are causing scientists to explore renewable and sustainable replacement fuel sources. One candidate is plant biomass, although without modification its energy-rich sugars remain unavailable to the standard industrial yeast Saccharomyces cerevisiae for fermentation to the fuel ethanol. In this …
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Development of a plant-made immunoassay for the detection of Porcine circovirus infections in South African swine herds
… cause of porcine circovirusassociated diseases and is one of the major pathogens in swine producing countries. PCV-2 is a non-enveloped virus with a single stranded circular DNA genome of about 1.8 kb. This encodes the single capsid protein (CP) which is highly immunogenic, as well as a …
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Engineering the Nicotiana benthamiana secretory pathway for the production of Lujo virus vaccines and diagnostic tools
… of viral pathogens. As seen with the Covid-19 pandemic, outbreaks of disease can be extremely detrimental to economies and put severe strain on the public health sector. Vaccination offers a solution to reduce the difficulties that accompany viral outbreaks. Lujo virus, an emerging arenavirus …
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Molecular characterization of Flavobacterium spp. isolated from Rainbow trout (Oncorhynchus mykiss) farmed in Southern Africa and development of a PCR-based tool for differentiating between isolates
… gill disease (F. branchiophilum, F. aquatile), and columnaris disease (F. columnare). Conventional diagnosis of Flavobacterium spp. is based on physico-chemical tests, but these tests are time-consuming and labor-intensive, and they are unable to distinguish between closely related species due …
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Modulation of the progesterone receptor by progestogens, antiretroviral drugs and the glucocorticoid receptor
Hormonal contraceptives (HCs) and hormonal replacement therapies (HRTs) are widely used globally by women. However, the relative activity of some of the progestogens used in HCs and HRT has not been determined via their target steroid receptor (SR), the progesterone receptors and its isoforms (PR-A …
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Towards the development of plant-made PsVs as potential delivery vehicles for therapeutic HPV vaccines
… cancer results in an estimated 604,000 new cases and 342,000 deaths each year, with the majority of these cases reported in sub-Saharan Africa. HPV-16 is a high-risk oncogenic subtype and, along with HPV-18, is associated with >70% of all cervical cancers. While current vaccines can prevent …
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Analysis of actinobacterial biodiversity in reservoir sediment and cave soil and screening of isolates for antimycobacterial activity
… Town), namely, cave soil, the wall of the cave and sediment from the shallow waters of a reservoir. Twenty nine (29) isolates were successfully identified to the genus level by 16S-rRNA gene analysis: one Micrococcus strain, one Streptacidiphilus strain, one Micromonospora strain and 26 …
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