Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Molecular Clones"”.
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Drug Resistance Mutations in Naive HIV-1 South African Patients, and Construction of Molecular Clones to Phenotype Putative Resistance Mutations
… of HIV replication. Two proviral chimeric clones making use of pMJ4 and pNL4-3, and two vector plasmids which deletions of sequences encoding HIV-1 protease or reverse transcriptase were constructed for cloning of HIV-1 PCR products. Growth of constructs was monitored by p24 antigen …
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To compare the expression, processing, incorporation and function of pseudoviruses and infectious molecular clones using different cell types and HIV backbones
… differential expression of recombinant Env clones across cell types, when pseudovirus and infectious molecular clone (IMC) backbones were introduced, expression of Env decreased. We also found differences in processing in the form of cleavage, N-glycosylation and incorporation of Env across …
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The role of HIV-1 subtype B Envelope transmission motifs in subtype C variant infectivity
… enhanced the entry efficiency of infectious molecular clones (IMCs), suggesting that it might be beneficial for in vivo replication. The variation between the PSV and IMC entry of TZM-bl cells could be due to differences in assay conditions. On the other hand deletion of PNG413 enhanced Env …
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The transmitted HIV-1 subtype C: characterization of the transmitted/founder full-length virus genome and the influence of early immune selective pressure on virus replication
… epitopes were introduced into infectious molecular clones of cognate transmitted/founder viruses by site-directed mutagenesis and their impact on viral replicative fitness was evaluated using parallel replication assays. In 77% of women (n=20) a single transmitted/founder variant …
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Investigating the susceptibility of foreskin myeloid cells to ex vivo HIV infection
… and chronic infection derived (CC) infectious molecular clones (IMCs) were used alongside Subtype B NL4-3 IMCs with CCR5, CXCR4 and BaL envelopes. The extent of HIV infection was quantified by measurement of p24 in different immune cell subsets over a time-course. The different HIV infected …
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Genetic and Biochemical Analysis of the Bryan Strain of Rous Sarcoma Virus
… BHRSV-transformed chicken embryo fibroblast clones were obtained and the provirus-cell junction fragments were mapped by restriction endonuclease cleavage and Southern blotting analyses. We found that expression of the viral genes could occur after proviral integration at many sites on the …
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Strategies for Oncogenesis by Avian Leukosis Viruses
… of this gene, resulting in neoplasia. Molecular clones of the provirus-host cell junctions (tumor junction fragments) from two independent ALV-induced lymphomas were isolated. The structures of these clones were compared with a clone of the normal c-myc gene. Restriction mapping …
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Differential effects of progestogens on HIV-1 replication and host gene expression in primary PBMCs and cervical tissue explants
… relevant to HIV-1 acquisition via differential molecular mechanisms is key to understanding choice of progestogen contraceptive in young women at high risk forHIV-1 acquisition. The central hypothesis of this study is that the differential effects on host gene expression and HIV-1replication by …
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Investigate the effect of Bacterial Vaginosis-associated bacteria on the efficacy of Anti-HIV therapy
… line, TZM-bl, during infection with infectious molecular clones and pseudovirus infection. Intracellular TDF, TFV, and MVC at t24 had no impact on HIV infectivity, suggesting that the concentration of ARVs internalised by the bacteria was not sufficient to inhibit infection to detectable levels. …
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Investigate the effect of Bacterial Vaginosis-associated bacteria on the efficacy of Anti-HIV therapy
… line, TZM-bl, during infection with infectious molecular clones and pseudovirus infection. Intracellular TDF, TFV, and MVC at t24 had no impact on HIV infectivity, suggesting that the concentration of ARVs internalised by the bacteria was not sufficient to inhibit infection to detectable levels. …
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Characterisation of HIV-1 subtype C envelope functional determinants of dual infected individuals
… were generated where gp41 was swapped between clones that either had the same (CAP84) or different (CAP267) PSV entry efficiency. For both participants, and (CAP137) gp41 was identified as a potential determinant of Env fitness. Moreover, two potential N-glycan sites (PNG) at position N332 and …
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The impact of neutralizing antibody and ADCC responses on HIV-1 envelope evolution in early infection
… we introduced select mutations into infectious molecular clones encoding the cognate early/acute envelope (Env-IMCs). In one participant, the mutation introduced conferred resistance to both nAb and ADCC responses, while in two participants, mutations were identified which resulted in resistance …
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Synergism between immune activators and progestogens: differential effects on gene expression and HIV-1 replication and the role of the glucocorticoid receptor
… were exposed to HIV-1Bal-Rellina infectious molecular clones (IMCs) before treatment progestogen alone or in combination with immune activators. In addition, TZM-bl cells were preconditioned with supernatants from PBCs treated alone with progestogen or in combination with immune activators …