Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"Molecular Biophysics"”.
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Molecular Biophysics of Mammalian and Fungal Macromolecular Assemblies
… for the collection of data reflecting the macromolecular organization of an ordered structure, and even intramolecular interactions of nuclei. This thesis details two projects that utilized NMR to gain a higher understanding of two macromolecular assemblies; each project has been divided into …
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Molecular Dynamic Simulation of Bio-molecular Dynamics, Folding, and Assembly
A major goal in molecular biophysics is to understand how biomolecules fold into specific structures to carry out cellular processes to result in a functioning organism. Computational molecular dynamics (MD) simulations can characterize biomolecules at a microscopic level. These indispensable …
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Development of a Yeast Transcription System Using a Saccharomyces Cerevisiae Enzyme Extract and a Drosophila Melanogaster Histidine tRNA Gene
… from Dieter Soll, Yale University Department of Molecular Biophysics and Biochemistry. In order to provide increasing amounts of the cloned gene, the bacterium Escherichia coli HB101 was transformed using standard transformation procedures and methods. The transformed bacteria were cultured, and …
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Towards understanding molecular, sub-molecular and atomic mechanisms of facilitated target search on DNA
… been the subject of considerable interest in molecular biophysics since the early 1970s. However, experimental and theoretical limitations have precluded the field from advancement in some key issues. For example, intersegment transfer on DNA scanning, interplay of distinct DNA scanning …
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Myosin structural dynamics: mechanistic insights and therapeutic technology developments
A major focus in molecular biophysics is to understand how protein structural isomerizations correspond to cellular and organismal physiology. The heart generates force to perfuse the body with oxygenated blood through contractile units in myocytes called sarcomeres. The primary force-generating …
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Blood-CNS transport mechanisms in pathophysiology and drug delivery
… barrier research<br/>with colleagues in Molecular Biophysics and Materials & Molecular Modelling has<br/>resulted in the creation of a ‘mini formulation-development pathway’ that can be<br/>utilised to optimise and develop formulations in the future.
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Molecular and Biochemical Analysis of the Histidine Kinase CusS and its Role in Metal Resistance in Escherichia coli
Transition metals such as copper, zinc and nickel are required in many enzymatic processes that require redox changes. When transition metal concentration exceeds a certain threshold, their redox and metal binding properties make these elements extremely toxic. Bacteria regulate the cellular …
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Moessbauer Studies of Cytochrome-P450 From Pseudomonas Putida
Made available in DSpace on 2015-05-12T22:39:07Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 7412185.PDF: 4019834 bytes, checksum: e4e5fb11b5fca0d3a3f6ddfc3371bfcb (MD5) Previous issue date: 1973
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Molecular genetic studies on the cytochrome bc(1) complex of Rhodobacter sphaeroides
Restriction data tranferred 2014-07-01T11:24:23-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission
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Relaxation dynamics in heme proteins
A protein molecule possesses many conformational substates that are likely arranged in a hierarchy consisting of a number of tiers. A hierarchical organization of conformational substates is expected to give rise to a multitude of nonequilibrium relaxation phenomena. If the temperature is lowered, …
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Moessbauer Study of Reduced Putidaredoxin in Zero and Intermediate Applied Fields
An ('57)Fe Mossbauer investigation of the reduced (S = 1/2) active center of the redox and effector protein, putidaredoxin, is reported. Putidaredoxin is a prototype of a 2Fe-2S protein; it functions as an electron shuttle in a bacterial enzyme system. Several 2Fe-2S proteins, including …
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Structural and Functional Studies of the Cytochrome D Oxidase Complex of Escherichia Coli
The cytochrome d oxidase complex is one of two terminal quinol oxidase complexes of Escherichia coli. The heterodimeric complex contains three heme prosthetic groups, $b\sb{558}, b\sb{595},$ and d. Two histidines, His19 and His186, in subunit I are essential for retaining the heme groups. His19 was …
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Biophysical Studies of Protein Assemblies
… interactions determines cellular fate at the molecular level. Characterisation of the model under simple biochemical conditions revealed large differences in affinities among binary interactions; the consequence of the lifetime of the complexes, not their speed of association. A membrane-like …
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Structural heterogeneity and conformational relaxation in heme proteins
The influence of cooling rate upon the structural heterogeneity of sperm whale myoglobin solutions at cryogenic temperatures was studied. Sample cooling rates were varied by almost four orders of magnitude. FTIR spectra of the CO stretch frequency region reveal that the population of the A states …
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Infrared-monitored flash-photolysis of carboxymyoglobin
An infrared-monitored flash-photolysis apparatus capable of measuring absorbance changes to 10$\sp{-3}$OD over a time range from 10$\mu$s to 10s is described. Measurements made on this apparatus, combined with slower measurements made on a Fourier-transform infrared spectrometer, of the …
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Theoretical and experimental studies on the structure of vertebrate visual pigments
High resolution three dimensional structure of visual pigments is essential for understanding the visual process. The past few years have been an exciting period in the field of the structure of visual pigments. One of the significant achievements is the 9A projection map of bovine rhodopsin. …
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Design and Construction of an Anti-HIV Protease Transducer
Current therapies against HIV, mainly the highly active anti-retroviral therapy (HAART), concentrate on reducing the viral load and recovering CD4 levels, however they are faced with a rebound of HIV levels on cessation of therapy. Also of concern is the number of reports on resistance to such …
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An investigation of processes that occur during the rebinding of carbon monoxide to myoglobin
The binding of small ligands to myoglobin at room temperature appears to be a simple, one-step process. However, experiments performed over a large range in time and temperature have shown that the situation is much more complex. Between 60 K and 160 K, the rebinding kinetics are non-exponential in …
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Understanding the Effect Of 1,3-Butadiene on Human Lung Fibroblasts: Does Exposure to 1,3-Butadiene Induce Senescence?
Cellular senescence is a state of irreversible growth arrest induced by either telomere shortening (replicative senescence) or telomere-independent signals (stress-induced premature senescence or SIPS). Recent studies have shown that cigarette smoke extract induces senescence in lung fibroblasts …
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The Rev-mediated Dimerization of HIV RRE RNA
The HIV-1 Regulator of Virion Expression (Rev) a regulatory protein which is critical for the late-phase development of the Human Immunodeficiency Virus-1 (HIV-1). During early-phase development of HIV-1, Rev accumulates in the cytoplasm and is transported into the nucleus through interactions with …
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