Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Modified Nucleotides"”.
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The utility of modified nucleotides for high-throughput nucleic acid analysis /
Synthetic base-modified nucleic acids are widely used in various fields. The aim of this dissertation was was to investigate the properties and utility of oligonucleotide-tethered 2′,3′-dideoxyribonucleoside 5′-triphosphates (OTDDNs), with emphasis on labeling of DNA and cDNA molecules for …
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Nmr Solution Structures Of Two Hairpins Of E. Coli 16s Rrna: The Effects Of Mutations And Chemical Modifications On Structure And Function Of Rrna
… 970 hairpin of E. coli 16S rRNA contains two modified nucleotides, m2G966 and m5C967. The two modified nucleotides in the 970 loop are involved in some important biological functions, for example, the m2G966 of the 970 hairpin interact with C34 of P-site bounded tRNA in the crystal structure. …
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Design, Off-Target Toxicity, and Intracellular Processing of Nucleotide-Modified Messenger RNAs for Human Therapies
… reduces innate immune activation compared to unmodified IVT mRNA. Despite this progress, there remain several potential issues with the use of modified nucleotides in mRNA therapy which may contribute to off-target and long-term toxicity. These include immunogenicity of IVT mRNA and off-target …
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Dna Aptamers Selected Against Wild-Type Helix 69 Ribosomal Rna And Their Implications In Combating Antibiotic Resistance
… conserved throughout phylogeny and rich in modified nucleotides, including pseudouridine () and 3-methylpseudouridine (m3) was chosen as a potential target. Helix 69, which is located in domain IV of Escherichia coli 23S ribosomal RNA (rRNA), undergoes conformational changes when in close …
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The mRNA Cap and Discontinuous Transcription in Trypansoma brucei
… where asterisks denote modification. The modified nucleotides in the cap were sequenced by a combination of partial and complete digests with non-specific nucleases. The four encoded, modified nucleotides are likely to have 2'-O-methylations, and possibly two other unidentified base …
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Studies on the detection of nucleotides and oligonucleotides by mass spectrometry
… is to develop novel methods for the detection of nucleotides, oligonucleotides, and modified nucleotides such as DNA adducts by mass spectrometry. DNA adducts are important because they are formed during chemical carcinogenesis as well as during anti-cancer chemotherapy. However, DNA adducts are …
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Role of Nudix-Hydrolase ASMTL in Modulating Mitochondrial Biogenesis of Cancer Cells
… pool during repair or DNA degradation. These modified nucleotides can then be incorporated into newly synthesized DNA, resulting in random mutations or, when overwhelming into DNA damage, culminating into apoptosis, which is the desired effect of anti-cancer chemotherapy. Human cells contain …
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Single-Molecule Characterization of RNA via Nanopore Sensing
… I track co-transcriptional incorporation of modified nucleotides in RNA. Overall, this thesis describes a method capable of reading full-length native transcripts, uncovering structural changes arising from alternative transcript processing, and identifying a variety of structural and …
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Investigation and application of methods for ancient DNA research
… an attempt to determine the identity of modified nucleotides in aDNA with Pacific Bioscience’s Single Molecule Real-Time (SMRT) sequencing prove to be unsuccessful and genotyping of ancient bison aDNA with the BovineSNP50 BeadChip generated inconsistent results. Furthermore, a …
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In vitro selection of deoxyribozymes for O-glycoside cleavage and for 3-nitrotyrosine modification
… functional groups on DNA using various modified dUTPs, which required a complicated synthetic preparation for each functional group. Chapter 2 describes the method of copper-catalyzed azide-alkyne cycloaddition (CuAAC) to install protein-like functional groups on alkyne-modified DNA. …
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Method Development And Applications To Screening And Characterization Of Rrna-Targeting Small Molecules
… aid in the ligand identification process, a modified FID assay for screening RNA-binding ligands was established using 3-methyl-2-((1-(3-(trimethylammonio)propyl)-4-quinolinylidene)methyl)benzothiazolium (TO-PRO) as the fluorescent indicator. ESI-MS results provide direct evidence that …
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Characterization of Yeast 18S rRNA Dimethyl Transferase, Dim1p
… numerous cleavages of pre-rRNA, modification of nucleotides, and concomitant assembly of the ribosomal proteins onto the rRNA. Considerable information is available about the importance of conserved pre-rRNA cleavage events in ribosome biogenesis; however, very little is known about the exact …
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Platination Kinetics: Insight Into Rna-Cisplatin Interactions As A Probe For Rna Microenvironments
… were selected. Two helix 69 (H69) constructs, modified H69 (with pseudouridine) and unmodified H69 (without pseudouridine), and the 790 loop, which has an identical size and nucleotide composition to unmodified H69, were used. Prior to kinetic studies, cisplatin targets on each RNA were …
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A systems biology approach unravels the biological function of two tRNA wobble base modifications in fine-tuning translation and the response to environmental stress
… produce several variations of the four canonical nucleotides. Despite the huge number of nucleotide modifications that have been identified in tRNAs, to date their biological roles and regulations continue to be poorly understood. The uridines at the wobble position of the eukaryotic cytoplasmic …
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Role of RNA methyltransferases in lung cancer
… to address whether other RNAs are indeed m²G modified by THUMPD3 and to further investigate and validate the role of the enzyme in lung cancer biology. Consistent with our bioinformatic data, depletion of THUMPD3 from lung cancer cells induced several notable effects; it negatively impacted …
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Next generation DNA-based data recorders
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01
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DNA enzymes for peptide-nucleic acid conjugation and for lysine methylation
… cycloaddition (CuAAC) with a 3'-alkyne-modified DNA oligonucleotide provides a substantial mass difference to allow PAGE separation of the catalytically active DNA. Selection experiments led to several deoxyribozymes that catalyze peptide-RNA conjugation of untethered peptides. Separate …
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DNA catalysts for amide bond cleavage and for lysine side chain modification
… that DNA can also form these structures. If oligonucleotides can act as comparable catalysts to proteins, a number of practical reasons to favor their development exist. The number of possible sequences is much smaller for DNA and RNA than for proteins; a sequence n units long has 4n possible …
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Novel molecular engineering approaches for genotyping and DNA sequencing
… of mass tagged, cleavable biotinylated dideoxynucleotides (ddNTP-N3-biotins) for DNA polymerase extension reaction and its application in DNA sequencing and single nucleotide polymorphism (SNP) genotyping by mass spectrometry. These nucleotide analogs have a biotin moiety attached to the 5 …