Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 94 for “"Mitotic spindle"”.
-
Regulation of mitotic spindle biogenesis in budding yeast
Assembly of the mitotic spindle is critically dependent on centrosome duplication (spindle pole body or SPB in yeast), separation of sister centrosomes and microtubule dynamics. Activation of Cdc28 (Cdk1) by tyrosine-19 dephosphorylation is essential for centrosome separation, the first step in …
-
Regulation of Mitotic Spindle Assembly in Caenorhabditis elegans Embryos
The mitotic spindle is a bipolar microtubule-based structure that mediates proper cell division by segregating the genetic material and by positioning the cytokinesis cleavage plane. Spindle assembly is a complex process, involving the modulation of microtubule dynamics, microtubule focusing at …
-
The Mitotic Spindle Mediates Inheritance of the Golgi Ribbon Structures
… reforms in both daughter cells after division. Mitotic Golgi membranes concentrate around the spindle poles, suggesting that the spindle may control Golgi partitioning. To test this, cells were induced to divide asymmetrically with the entire spindle segregated into only one daughter cell. A …
-
Molecular Mechanisms of Mitotic Spindle Assembly and Accurate Chromosome Segregation
… proteinaceous structures built on centromeres of mitotic chromosomes that act as the attachment site for microtubules, serve as major forces to position each pair of chromosomes to the metaphase plate. Secondly, a surveillance system, known as the mitotic checkpoint, put the anaphase onset on hold …
-
Identification and Analysis of A Novel Role For The to usled-Like Kinase In Regulating Mitotic Spindle Dynamics
… by poor metaphase chromosome alignment, delayed mitotic progression, lagging chromosomes, and supernumerary centrosomes.</p> <p>I discovered an unanticipated requirement for TLK-1 in mitotic spindle assembly and positioning. Normally, in the newly-fertilized zygote (P<sub>0</sub>) the maternal …
-
B-cyclin/CDK Regulation of Mitotic Spindle Assembly through Phosphorylation of Kinesin-5 Motors in the Budding Yeast, <italic>Saccharomyces cerevisiae</italic>
… complexes regulate the assembly of the mitotic spindle and entry into mitosis, the full complement of relevant CDK targets has not been identified. It has previously been shown in a variety of model systems that B-type cyclin/CDK complexes, kinesin-5 motors, and the …
-
Identification and characterisation of FAM83 proteins as key regulators of CK1 protein kinases in cells: FAM83D recruits CK1α to the mitotic spindle for proper spindle positioning
… the only FAM83 member known to localise to the mitotic spindle. RNA interference approaches targeting FAM83D have been reported to impact chromosome alignment and cause a delay in the timings of cell division. Whilst all FAM83 proteins appear to associate robustly with the CK1 alpha isoforms …
-
SMARCA4/BRG1-Inactivating Mutations as Potential Predictive Markers for Aurora Kinase A-Targeted Therapy in NSCLCs
… Thus, AURKA-centered, centrosome-independent, mitotic spindle assembly machinery becomes solely responsible for mitotic spindle formation and proper chromosome segregation during mitosis. DLG7, the only known protein specific to this centrosome-independent mitotic spindle assembly, is required …
-
Regulation of microtubule attachment at the kinetochore and cell cortex
… function of complex organs, such as the brain. Mitotic chromosome segregation failures and the resulting DNA damage is also a hallmark of human cancers. There are multiple organelles that must work together to acheive a successful mitosis, including the chromosomes, kinetochores, centrosomes, …
-
Regulation of Progression through Unperturbed Mitosis and in the Presence of Environmental Stress
… of the genome. Surveillance pathways like the mitotic spindle checkpoint have evolved to prevent mitotic defects effectively. The molecular basis of these regulatory mechanisms is particularly well understood in budding yeast. It is not entirely clear to what extent these findings are valid for …
-
Kinetochore-Microtubule interface: analyses of Ndc80-Dam1 Interactions
… kinetochores and align sister chromatids on the mitotic spindle. Initially kinetochores interact with the lateral surface of a microtubule (lateral attachment), which is subsequently converted to an end-on attachment when the tip of a shrinking microtubule reaches the kinetochore.<br/><br/>The …
-
Molecular Mechanisms Involved in Chromosome Segregation
… and couples it to the microtubules (MTs) of the mitotic spindle. In human cells, the details of CCAN-CENP-ANuc assembly are unknown. This thesis investigates the structure and assembly of the human CCAN-CENP- ANuc complex, showing that CCAN tightly grips the linker DNA of the CENPANuc. CENP-C …
-
The Effects of RHAMM on Cell Division, Cell Motility, and Tumorigenesis
… are not well understood. RHAMMΔ163 binds to the mitotic spindle and centrosomes via a C-terminal leucine zipper; these interactions are predicted to regulate genomic stability and cell polarity and proposed to account for the oncogenic function of RHAMMΔ163. We hypothesized that RHAMM leucine …
-
DECIPHERING THE FUNCTION OF HURP IN REGULATING MICROTUBULE DYNAMICS AND CHROMOSOME MOVEMENT
The regulation of mitotic spindle dynamics is important for faithful chromosome segregation. HURP is an important microtubule-associated protein for stabilizing and bundling the mitotic spindle. Depletion of HURP delays the mitotic progression, but the underlying mechanism remains unknown. Our …
-
Using Microscopy to Examine Mechanisms of Cell Division
… requires the assembly of a microtubule-based mitotic spindle. In the research presented here, I examined two aspects of mitotic spindle morphogenesis. First, I looked at how chromosomes attach to microtubules in mitosis. The capture of microtubule plus-ends by kinetochores (the site of …
-
LOCALIZED WNT3A-SOURCES INSTRUCT MITOTIC ORIENTATION: DISSECTING THE WNT3A-TRANSDUCTION MECHANISM COUPLED WITH NUMA MACHINERY
… which rely on the proper positioning of the mitotic spindle. Consistently, defects in epithelial polarity and spindle orientation are often coupled with loss of tissue architecture and altered proliferation. Since mitotic spindle orientation determines the axis of cell division, in stem cells …
-
ANALYSIS OF TWO NOVEL COMPLEXES THAT REGULATE NEUROBLAST SELF-RENEWAL AND ASYMMETRIC DIVISION IN DROSOPHILA MELANOGASTER
… and cell fate determinant Numb, as well as mitotic spindle orientation in neuroblasts. The other player is Drosophila cytoplasmic dynein light chain 1, also named Cut up (Ctp). Ctp localizes at centrioles and controls the mitotic spindle orientation in neuroblasts. Ctp directly interacts …
-
Self-organization of Microtubule and Associated Protein Map65
… microtubule bundle that is reminiscent of the mitotic spindle. I characterize these 'tactoids' and show that these are homogeneous and jammed inside. Lastly, I present results of the MAP65 condensate formation via liquid-liquid phase separation and quantification of their properties. …
-
Spindle organization in three dimensions
… takes place on bipolar, microtubulebased spindles. Here, C. elegans is used to analyze spindle organization under both mitotic and meiotic conditions. First, the role of SAS-4 in organizing centrosome structure was analyzed. Partial depletion of SAS-4 in early embryos results in …
Page 1 of 5