Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 94 for “"Mitochondrial membrane potential"”.
-
Influence of mitochondrial membrane potential on the cryopreservation survival of hepatocytes
… agents and optimizing cooling rates. Membrane-permeable cryoprotectants, such as dimethyl sulfoxide, though widely used, can be extremely toxic to the cell. More natural, non-membrane-permeable cryoprotectants, inspired by freeze-tolerant animals have also been used. A …
-
Development of Nanodiamond and Raman Spectroscopy Sensing Methods towards the Measurement of Mitochondrial Membrane Potential in Single Cells
Mitochondrial membrane potential ($\Delta\Psi_{\textrm{m}}$) in cells is a critical biological parameter that is related to diseases such as cancer through biological processes including metabolism, oxidative stress and apoptosis. Studying $\Delta\Psi_{\textrm{m}}$ in normal and diseased states is …
-
Metaboloptics: In Vivo Optical Imaging to Enable Simultaneous Measurement of Glucose Uptake, Mitochondrial Membrane Potential, and Vascular Features in Cancer
… by providing information about tumor metastatic potential. Metabolism can further be used to guide treatment selection and monitoring, as cancer treatments can influence metabolism directly by targeting a specific metabolic dysfunction or indirectly by altering upstream signaling pathways. …
-
5 ns Electric pulses evoke longer-lived calcium responses in adrenal chromaffin cells than the physiological stimulus: an experimental and computational study
… influx through VGCCs, and a disruption of mitochondrial membrane potential has been shown to delay calcium clearance, our first aim was to explore whether 5 ns pulses affect mitochondrial membrane potential using fluorescent mitochondrial membrane potential dyes. Control experiments …
-
Early hippocampal dysfunction in a mouse model of dementia with Lewy bodies: Mechanisms of cellular dysfunction caused by alpha-synuclein accumulation
… DLB-specific mouse model, focusing on changes in mitochondrial function. Additionally, I explore the role of the wild-type and a DLB-related mutation of the protein beta-synuclein on mitochondrial function. The novel mouse model, known as CamS120, expresses a truncated form of the human …
-
Novel Synthetic Compound, KR-22332, has radio-protective effect through inhibition of Mitochondrial Dysfunction and ROS Generation
… reactive oxygen species generation, and mitochondrial membrane potential as well as changes in the signal pathway related to apoptosis were investigated. Then, the embryotoxicity of KR-22332 were explored in a zebrafish model. Radiation-induced apoptosis and altered mitochondrial membrane …
-
Do Elevated Levels of Glutathione Decrease the Toxicity of Plasticizers in Mouse Liver Cells?
… observing cell viability, ATP production, mitochondrial membrane potential, and reactive oxygen species (ROS) production. Results indicate significant toxic effects of DEHP and MEHP from cell viability and ATP assessments and show that the two cell lines respond differently to the different …
-
Effect of Quantum Dots, Novel Biological Imaging Agents, in Liver Cells in Vitro
… stability in aqueous environments make them potentially useful for such applications as imaging and ligand attachments. However, there are concerns regarding the toxic effects of QDs and the minimal dose that can be used without producing any detrimental effects to organisms. In this study, …
-
A chemical genetic approach to identify new treatments for melanoma
… for rheumatoid arthritis patients also holds potential therapeutic value in treating melanoma. This thesis shows the results of a new successful chemical genetic and cell based viability screen of the NCI Diversity set II library. 13 potential novel targets for treating melanoma were …
-
Metabolic Mechanisms of Nutritionally Regulated Hormone Signaling on T Helper Cell Function
… Furthermore, IGF-1 treatment decreases mitochondrial membrane potential and mitochondrial ROS (mROS) production in Th17 cells, presumably to protect the cells from oxidative stress. Interestingly, both IR and IGF-1R appear to be required for this effect. This could indicate that the …
-
The role of Biophotons in cellular senescence.
… cellular metabolic processes, including mitochondrial respiration, are known to emit ultra-weak photons (biophotons), primarily generated through ROS production. There is increasing evidence that cells can influence each other non-chemically via biophotons. In this study, I aim to …
-
Cardioprotective Effect of Onion Extract against Ischemic Injury In vitro and In vivo
… including garlic and onion, are known to have a potential to reduce the detrimental effect of cardiovascular risk factors, and their beneficial roles are suggested to be associated with antioxidant mechanism. Compared to garlic, very few have been reported about the myocardial beneficial effect …
-
Evaluierung durchflusszytometrischer Verfahren zur Beurteilung der Qualität von kryokonserviertem Hengstsperma
… cryopreserved sperm from stallions. The plasma membrane integrity, the mitochondrial membrane potential, the acrosomal status and the integrity of the chromatin structure in cryopreserved sperm from stallions were assessed. For this purpose various fluorescent stainings were carried out both …
-
Oxidative Stress Based Strategies For Enhancing The Efficacy of Histone Deacetylase Inhibitors (Hdaci)
… LEHD. In addition, we showed that loss of the mitochondrial membrane potential is the earliest event observed starting at 12 h. Following this event, we observed increased levels of superoxide at 16 h, and ultimately caspase-3 activation. Pre-treatment with the antioxidant N-acetylcysteine …
-
Transcription Regulates Biased Mitochondrial DNA Inheritance
Maintaining mitochondrial DNA (mtDNA) is essential in eukaryotes for cellular respiration and ATP production by oxidative phosphorylation. Respiration is the preferred method for creating mitochondrial membrane potential, which is important for the import of nuclearly encoded proteins into the …
-
Astrocytes Rescue Neuronal Health After Cisplatin Treatment Through Mitochondrial Transfer.
… health after cisplatin treatment through mitochondrial transfer.</p> <p>Author: Krystal English</p> <p>Advisory Professor: Dr. Cobi J. Heijnen, Ph.D.</p> <p>Chemotherapy-induced cognitive impairments are associated with neuronal mitochondrial dysfunction. Cisplatin, a commonly used …
-
Drp1 inhibition is protective against mitochondrial and autophagic impairment induced by alpha-synuclein
… in PD pathology, it has been found to induce mitochondrial dysfunction, yet the mechanism is not entirely understood. This thesis project tests the hypothesis that α-syn induces mitochondrial dysfunction through disruption of fission/fusion pathway. Using an inducible cell line, I successfully …
-
The Role of Peroxiredixin Ⅲ in the ototoxic drug-induced mitochondrial apoptosis of cochlear hair cell
… cells. Among them, Prx III is synthesized with a mitochondrial targeting sequence and is then transferred to mitochondria. We have shown that Prx III is an important, if not the most important, H2O2-eliminating enzyme in mitochondria and that the mitochondrial generation of H2O2 contributes to …
-
The Influence of Cellular Redox State on Mitosis
… regulator, Aurora kinase B, by changes in mitochondrial metabolism during mitosis. In early mitosis, an increase in mitochondrial membrane potential coincides with a robust reduction in cellular redox status, preventing disulfide bond-mediated activation of Aurora kinase B as a determinant …
-
On connections between metazoan cellular metabolism and cell size
… This revealed that the relative expression of mitochondrial and lipid biosynthesis genes are downregulated with increasing cell size. However, mitochondrial content of the liver samples was not decreased, suggesting that cell functions and cell contents scale differently with cell size. To …
Page 1 of 5