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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Mitochondrial enzyme"”.
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Studies on Type I and Type II DNA Topoisomerases From Mammalian Mitochondria
… and bacterial type I DNA topoisomerases. This enzyme, purified from bovine liver, requires cations as cofactors and possesses optimal activity at pH 7.0, 37°C and either 150 mM K+ or Na+ ions. Similar to nuclear enzymes and different from the bacterial enzyme, divalent ions such as …
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A characterization of chicken heart mitochondrial malate dehydrogenase
Chicken heart mitochondrial malate dehydrogenase has been purified by an improved isolation method to give 56% of the initial mitochondrial enzyme. The purified enzyme has a specific activity of 340 U/mg. This homogeneous enzyme has been shown to be pure by several criteria. The enzyme has been …
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Biomechanical and physiological investigations in the IBMPFD animal model
… old VCPR155H/+ mice revealed significantly lower mitochondrial enzyme activity (citrate synthase) in VCPR155H/+ mice at 14 months; concomitant with the reduction in fasttwitch fibre fatigue tolerance. Primary fibroblast cells isolated from our VCPR155H/+ mouse model showed reduced ability of …
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Effect of Methamphetamine on Gingival Fibroblast Production of Matrix Metalloproteinase (MMP)-2 and -9 and Tissue Inhibitor of Metalloproteinase (TIMP)-1 and -2 In Vitro
… by measuring its effects on activity of a mitochondrial enzyme. Cells were cultured ± METH (1x10<sup>-6</sup>- ≥ 1x10<sup>-3</sup>M); MMP-2, MMP-9, TIMP-1,TIMP-2 and MMP:TIMP complexes in cell supernatants were measured by ELISA. MMP-2 activity was measured using the MMP-2 Biotrak Activity …
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Responses of Superoxide Dismutases to Oxidative Stress in Arabidopsis thaliana
… Fe SOD protein. Effects of oxidative stress on enzyme activity were measured in native gels. Fe SOD responded to oxidative stress with an increase in activity, but not in antibody reactive protein. Two novel forms of Fe SOD activity, with faster migration rates in activity gels, were detected. …
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Generation of a Medaka Fish Model of Propionic Acidemia for Development of Novel Therapies.
… error of metabolism caused by deficiency of the mitochondrial enzyme propionyl-CoA carboxylase (PCC). The disease presents with acute, recurrent and life-threatening crises of metabolic decompensation starting from the newborn period. Patients also suffer from multi-organ complications, …
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An Analysis of Mitochondrial DNA in Rett Syndrome and Other Neurodegenerative Disorders
<p>Mitochondrial dysfunction resulting from mutations on mitochondrial DNA (mtDNA) is being recognized in a growing spectrum of diseases. These diseases, resulting from single base mutations, large deletions, or insertions, have been largely neuromuscular in origin. However, as an understanding of …
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Investigation of the Substrate Recognition Characteristics and Kinetics of Mammalian Mitochondrial DNA Topoisomerase I
<p>Topoisomerases are DNA-modifying enzymes found in prokaryotes, eukaryotes, viruses and organelles such as chloroplast and mitochondria. Information about these enzymes in eukaryotic systems is mostly limited to nuclear enzymes, although our laboratory has been characterizing the biochemical and …
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Influence of biological sex and thyroid hormone on skeletal and cardiac muscle metabolism in heat-stressed pigs
… be more MF than gilts even though no changes in mitochondrial enzyme activity were observed. However, gilts differed between environmental treatments regarding CS activity (P=0.03). Blood serum from the treated pigs was placed on skeletal muscle cell cultures. Metabolic flexibility between the in …
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The role of cardiolipin in the regulation of mitochondria-dependent apoptosis
… step, involving the permeabilisation of the mitochondrial outer membrane to apoptogenic factors, such as cytochrome c and Smac/DIABLO. The release of these factors into the cytosol is tightly regulated by proteins of the Bcl-2 family and results in the activation of the caspase cascade, …
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Shared Metabolic Pathways in Fuel-Stimulated Insulin Secretion
… in insulin secretion, we also investigated the mitochondrial enzyme glutamate dehydrogenase (Glud1). Overexpression of wild-type Glud1 failed to alter insulin secretion in 832/13 cells or in islets; however, suppression of Glud1 decreased both GSIS and AASIS, but did not affect …
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Statins Regulate IL-1β - Induced RANKL and OPG Production by Human Gingival Fibroblasts
… using an assay that measures the activity of a mitochondrial enzyme. Fibroblasts were preincubated with atorvastatin or simvastatin for 24 hours in serum-free medium, and then incubated without a stimulus or with IL-1β for 6 days. OPG or RANKL in culture supernatants was measured by specific …
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Mechanistic Characterization of the Mitochondrial Type I DNA Topoisomerase and a Study of Genes Containing Type I DNA Topoisomerase-Related Domains
<p>The mitochondrial type I DNA topoisomerase (mt-topo I) serves an important function in the mitochondrion by relaxing mtDNA supercoils to allow for replication of the mitochondrial genome as well as for gene expression. The mt-topo I's role in essential processes, such as replication and …
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Endocannabinoid and lipid metabolism genes network analysis in adipose and liver tissue of dairy cows during the transition period
… qPCR of endocannabinoid receptors (CNR1, CNR2), enzymes that synthesize endocannabinoid (NAPEPLD), enzymes that degrade endocannabinoids (FAAH, NAAA, MGLL), and the hormone precursor proopiomelanocortin (POMC). We also examined mRNA expression via qPCR of genes associated with lipolysis (LIPE, …
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In vitro investigation of the molecular relationship between mitochondrial dysfunction and neurogenesis in autism aetiology
Mitochondrial function is integral in the regulation of neurogenesis. Our research group previously reported differentially methylated genes and metabolomic evidence converging on mitochondrial dysfunction in a South African autism spectrum disorder (ASD) cohort. Thus, subtle mitochondrial …
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The interaction of hepatitis C virus and intracellular lipid metabolism
… showed differential regulation of genes encoding enzymes concerned with lipid metabolism. Treatment of HCV genotype 1b replicon containing cells with cerulenin, which inhibits fatty acid synthase, reduced replication of HCV RNA in a dose dependent manner (Su et al., 2002). Polyunsaturated fatty …
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Identification and characterization of genetic markers and metabolic pathways controlling net feed efficiency in beef cattle.
… feed intake in Lirnousin x Jersey animals, b) mitochondrial oxidative phosphorylation enzyme assays in high and low NFE cattle, and c) 2-dimensional fluorescent gel electrophoresis (DIGE) proteomics analysis of mitochondrial proteins. For the cattle QTL mapping, the results from a previous …