Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"MitoTracker"”.
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THE ROLE OF SKELETAL MUSCLE-SYNTHESIZED BRAIN DERIVED NEUROTROPHIC FACTOR IN THE MAINTENANCE OF MOTOR NEURON MITOCHONDRIAL POPULATIONS
… received intramuscular injections of MitoTracker™ dye targeting the right gastrocnemius muscle. At 120d experimental groups underwent a gastrocnemius harvest or a sciatic nerve ligation protocol prior to sacrifice. In combination with MitoTracker™ injections, we used …
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Investigating putative pathogenic mechanisms within a family in which a chromosomal translocation confers risk of major mental illness
… of the cell. Initial staining attempts with MitoTracker Red to visualise mitochondria in CP60 and CP69 expressing cells resulted in fewer than 30% of cells being stained. In those that did stain, the mitochondria appeared clustered. The absence of MitoTracker Red staining in mitochondria may …
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Light in Living Systems: Ultra-Weak Photon Emission and Fluorescence Lifetime Imaging of Cellular Dynamics
… nano-organisation. Dual staining with MitoTracker^(TM) Green FM (MTG) (donor) and MitoTracker^(TM) Orange (MTO) (acceptor) produced consistent lifetime shifts and phasor displacements e.g. ∆r ≈ 0.030), with global efficiencies of 10.7% and 20.9% corresponding to donor acceptor …
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Burn Serum Stimulates Mitochondrial Fission in C2C12 Myoblasts
… or control rats. Cells were then labeled with MitoTracker Green dye and live cell images were recorded by confocal microscopy. The expression of mitochondr ial fission/fusion factors was examined by Western blots. RESULTS: In cells cultured with 10% serum from control rats, mitochondrial …
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Effects of Polydatin on In Vitro Bovine Embryo Developmental Competence, Metabolism, and Cryopreservation
… were stained with Nile Red for lipid content or Mitotracker Red CMX-Rosamine for mitochondrial activity or Cell Rox Green for reactive oxygen species. Ten images per embryo were acquired using confocal microscopy at 5-µM step size and detected fluorescence by IMAGE PRO software. Embryos were also …
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THE ROLE OF SKELETAL MUSCLE-SYNTHESIZED BRAIN-DERIVED NEUROTROPHIC FACTOR IN RETROGRADE TRANSPORT ALONG MOTORNEURON AXONS
… presynapse, we injected the mitochondrial dye MitoTracker into the gastrocnemius muscle of 120d transgenic animals and measured colocalization of this dye with presynaptic and postsynaptic immunofluorescent markers. Our results reveal a significant decrease in mitochondria at the presynaptic …
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Investigating the Role of msBDNF – TrkB Signaling in the Maintenance of Mitochondrial Populations Through the BNIP3 Mitophagy Pathway
… Injections of the mitochondria-labeling dye MitoTracker were performed on 52-day-old and 87-day-old mice in order to determine if abnormalities in mitochondrial clearance are a cause of the pathophysiology observed in this model, or possibly a byproduct of it. A BNIP3-specific antibody was …
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Relationship between Hepatitis B Virus Core Particles and Intracellular Motility
… staining. Mitochondria were stained by MitoTracker. And microtubules (MTs) was visualized using anti-tubulin antibody. RESULTS: The mitochondrial aggregation towards the nucleus was observed in HBV replicating stable cells, but not in HuH7 and HepG2 cells. When HBV replicating stable …
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Inhibition of Mitochondrial Translation as a Therapeutic Strategy for Acute Myeloid Leukemia
… Flow cytometry dye; JC-1, Carboxy-H2DCFDA, Mitotracker GreenFM. Leukemia stem cell activity was assayed by human AML engraftment in NOD/SCID mice. Results: TIG induced cell death in primary AML patient samples (LD50, 3-6μM n=14), preferentially over normal hematopoietic cells. Likewise, in …
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Age-Related Hamster Mitochondrial Changes and Oocyte Changes Following Autologous Platelet Mitochondrial Microinjection
… to blastocyst stage were not improved. MitoTracker Green FM (MGF) was used to help quantify mitochondria, but the results conflicted with mtDNA and TEM data, which suggests that MGF, as a MMP dependent reagent, is not an ideal method to reflect mitochondrial number. Our data suggest that …
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Microdevices for Isolating Single Axons for the Study of Axonal Transport in Dopaminergic Neurons
… vector or with a mitochondria specific dye, Mitotracker Deep Red. While both methods successfully labeled the mitochondria, the dye, created significant fluorescent background noise which made measuring mitochondria movement difficult. Using the microdevice, the effects of 6-hydroxydopamine: …
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Mitochondrial dynamics in the radiation response of cancer cells
… respiration and membrane potential using MitoTracker® Red staining, respirometry and JC-1 ratiometric staining, respectively. Correlations with radiation sensitivity were performed. Radiation- and Mdivi-1-induced changes in mitochondrial morphology were also examined. Responses to various …
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Investigation of the mechanisms underlying the effects of hyperglycaemia on cardiac structural and electrical remodelling
… did not alter the mitochondrial staining with Mitotracker. It also increased the oxidative stress marker nitrotyrosine but did not alter the extent of EdU nuclear staining nor the expression of the receptor of advanced glycation end-product. The antioxidant n-acetyl cysteine decreased the …
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AMP Deaminase 3 overexpression regulates cellular energetics and signaling for PGC-1α activation.
… for mitochondria using a green fluorescent dye (MitoTracker green FM) and quantified the percentage of pixels positive for florescence. Results: DNP treatment resulted in a 40% decline in [ATP], and increased [ADP] (1.4-fold), [AMP] (13.8-fold), AMP:ATP ratio (24-fold), and [IMP] (from …
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Maturation and Culture Media Effects on In Vitro Bovine Embryo Developmental Competence
… stained with Nile Red to evaluate lipid content, Mitotracker Red CMX-Rosamine to measure mitochondrial activity, or Cell Rox Green to assess reactive oxygen species (ROS). Using a confocal microscope, images were taken of each stained embryo to detect and measure fluorescence. Any stage 7 embryos …
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Characterization of Cytotoxicity against Cancer Cells induced by Human Monoclonal Antibody Specific to TRAIL-R1
… after Hoechst33342 (nucleus) and Mitotracker (mitochondria) staining. Also we tested combination effect, Jurkat cells were treated with DR4-4 Fab and r-irradiation simultaneously and cell death was measured by flow cytometry after Annexin-V/PI staining. RESULTS: The DR4-4 Fab …
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Mechanism of Action Studies of Phenotypic Whole-cell Active Antimalarial Leads Through Target Identification
… colocalization was not observed between the MitoTracker Deep Red and KP68, significant amounts of the compound localize around the mitochondrion membrane. Finally, all compounds were assessed in the cellular heme fractionation assay. Results from this assay indicate that inhibition of …