Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"Mislocalization"”.
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AUDITORY CUES AND RESPONSE MODES MEDIATE PERIPHERAL VISUAL MISLOCALIZATION
… of peripheral visual stimuli often leads to mislocalization (Binda, Morrone, & Burr, 2010; Bocianski, Musseler, & Erlhagen, 2008; Musseler, Heijden, Mahmud, Dubel, & Ertsey, 1999) these types of stimuli are the most commonly studied and represent the basis of the current study. Musseler et …
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A role for RNA localization in the human neuromuscular disease myotonic dystrophy
… distribution of the effected message. DMPK mRNA mislocalization causes altered DM kinase protein localization, correlates with novel phosphoprotein appearance and can account for DM’s diseased phenotype. While we were fortunate to access DM patient tissue to establish these key findings, the …
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Direct Effects of Metformin On Pi3K and Ras Signaling In Endometrial Cancer
… reduced sensitivity to metformin.</p> <p>Mislocalization of K-Ras to the cytoplasm is associated with decreased signaling and induction of apoptosis. Metformin’s effect on K-Ras localization was analyzed by confocal microscopy in cells expressing oncogenic GFP-K-RasG12V. Metformin …
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AGGREGATION MECHANISMS OF TDP-43 PROTEIN IN RESPONSE TO STRESS IN AMYOTROPHIC LATERAL SCLEROSIS AND THERAPEUTIC APPROACHES
… proteinopathy, recapitulating TDP-43 cytoplasmic mislocalization and loss of nuclear function, to be used for drug screening approaches. We induced a chronic and mild oxidative insult by sodium arsenite (ARS) in human neuroblastoma SK-N-BE cells and ALS patient-derived primary fibroblasts, induced …
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Investigating The Role of Pancreatic eIF2-alpha kinase (PEK) In DLK/ Wallenda (Wnd)-mediated Neurodegeneration In Drosophila
… intrinsic and extrinsic stressors, and results mislocalization and aggregation of these proteins occurs, subsequently resulting in functional and structural alterations in neurons, and leading to neuronal dysfunction and death, the characteristic features of neurodegenerative diseases. The …
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CAG repeat expansions induce cytoplasmic RNA aggregation
… cytoplasmic RNA aggregation, cytoplasmic mislocalization of RNA-binding proteins, as well as toxicity. In the second part of this thesis, I present evidence that the cytoplasmic repeat RNA aggregates also contain NEAT1, a lncRNA that typically localized to the nucleus. CAG repeat RNAs that …
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The Non-canonical Function and Regulation of TBK1 in the Cell Cycle
… FIP200 or ATG9A exhibit overactivation and mislocalization of TBK1. By using both genetic and pharmacological inhibition of autophagy processes, we found that impaired autophagy leads to a significantly higher number of micronuclei – a hallmark for tumorigenesis that correlates with defects …
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Regulation of lipid signaling at the Golgi by the lipid phosphatases hSAC1 and OCRL1
… and endosomal like structures and causes mislocalization of ManII and NacT1. This data suggests that SAC1 establishes PI(4)P-depleted Golgi regions that are important for proper localization and recycling of Golgi resident enzymes. Conversely, depletion of OCRL1 does not disturb Golgi …
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DEFINING THE ROLE OF THE PCGF3-CONTAINING POLYCOMB REPRESSIVE COMPLEX 1 IN SYNOVIAL SARCOMA
… repressive domains, thus activating them. This mislocalization depends on the higher affinity of the fusion oncoprotein for H2AK119ub, the histone modification deposited by Polycomb Repressive Complex 1 (PRC1). However, the precise molecular mechanisms underlying SS18-SSX transformation remain …
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Targeting Plasma Membrane Phosphatidylserine Content to Inhibit Oncogenic Kras Function
… reduced PM PtdSer levels, resulting in extensive mislocalization of KRAS from the PM. Concordantly, ORP5 or ORP8 depletion significantly reduced proliferation and anchorage-independent growth of multiple KRAS-dependent cancer cell lines, and attenuated KRAS signaling <em>in vivo</em>. Similarly, …
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Inactivation of Tor Proteins Affects the Dynamics of Endocytic Proteins in Early Stage of Endocytosis
… decreased. In agreement with the Sjl2 mislocalization, I found that in tor1∆tor2ts double mutant cells, as well as other tor mutant cells, the overall PIP2 level was dramatically increased. Finally, the cell wall chitin content in tor2ts and tor1∆tor2ts mutant cells was also …
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Genetic analysis of the maintenance of neuronal morphology in Drosophila melanogaster
… microtubule motor protein Dynein, causes mislocalization of photoreceptor nuclei basally, even into the optic stalk and brain. Photoreceptors in animals mutant for the Dynactin subunit Glued retain apical markers, but have a bipolar-like morphology with the cell body translocated toward …
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The Function of Phosphatidylinositol 4-Kinase III-Beta in Trypanosoma Brucei
… studies revealed a distorted Golgi apparatus and mislocalization of lysosomal and flagellar pocket proteins. Ultrastructural analysis reveals internal accumulation of a heterogeneous population of vesicles, abnormal positioning of organelles and a loss of cell polarity. Scanning EM reveals a …
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The Role of Presynaptic Par-1 Kinase in Regulating the Localization of Active Zone Protein BRP
… data suggest an intriguing possibility that mislocalization of active zone proteins like BRP might be one of the earliest signs of perturbation of synapses that precede many neurological disorders.
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Understanding The Visual And Auditory Defect In Ush2a Mouse Model
… in-depth analysis found that this was due to the mislocalization of the KI mutant protein and a subsequent mislocalization of its other USH2 interacting partners. Along with patients exhibiting phenotypes associated with homozygous mutations of USH2A, there is also a prevalence of cases involving …
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Kap-[beta]2 Recognition of Cargoes Involved in Neurodegenerative/Neurodevelopmental Diseases
… We have observed that these mutations cause mislocalization of the cargo to stress granules in the cytoplasm. I performed biophysical and structural studies and found that the HNRNPH2 has a new type of epitope not observed before. Furthermore, I observed that this new epitope is essential for …
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Examining Intracellular Phosphorylation Gradients During Cell Division
… and persists until cell cleavage. Selective mislocalization of the CPC during anaphase suppresses gradient formation, but overall substrate phosphorylation levels remain unchanged. Under these conditions, the spindle midzone fails to organize and function properly. My findings suggest a model …
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Function of a Unique Dually Localized EF-Hand Domain Containing Protein, TgEFP1, During the Lytic Cycle of the Human Parasite Toxoplasma Gondii
… treatment. Signal peptide deletion led to a mislocalization of TgEFP1 as cytosolic puncta, while mutations at key calcium coordinating residues lead to exclusive localization of TgEFP1 at the PV. Lastly, immunoprecipitation assays followed by LC-MS/MS identified a novel lectin-like protein- …
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Interaction between DVAP, the orthologue of human VAPB, and Drosophila Sac1
… to have a dominant negative effect on Sac1. Sac1 mislocalization could inhibit the dephosphorylation of PI4P. This PhD work further confirms Sac1-DVAP interaction and it suggests a mechanism underlying ALS8 pathogenesis, supporting the idea that altered metabolism of phosphoinositides can cause …
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Functional characterization of the role of Imp, a Drosophila mRNA binding protein, during oogenesis
… of Imp in germ line cells results in mislocalization of grk mRNA and protein. The opposing effects of reduced and elevated Imp activities on grk expression suggest that Imp acts in regulation of grk expression, but in a redundant way. To further explore the mechanisms by which …
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