Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Minus-end"”.
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Regulation of Microtubules by Patronin within Drosophila Dendrites
… all cells, comprising of a cell body, axon and dendrites. The development and function of each compartment relies heavily on the cytoskeleton, a major component of which are microtubules, which are required for intracellular transport, as well as establishing neuronal morphology. In this thesis, …
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Genetic analysis of the maintenance of neuronal morphology in Drosophila melanogaster
… located apically in the epithelium, and axons extending basally into the brain. Disruption of the Dynactin complex, which activates the minus-end-directed microtubule motor protein Dynein, causes mislocalization of photoreceptor nuclei basally, even into the optic stalk and brain. Photoreceptors …
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Force studies of intracellular transport
… Dictyostelium cells typically have 1 active plus-end directed motor and 1 active minus-end directed motor. Also, the plus-end motor’s stall force appears to be lower (2-3 pN) than kinesin-1’s in vitro stall force (5-7 pN), while the minus-end motor’s stall force (2 pN) is slightly higher but …
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Bidirectional transport by molecular motors
… an intrinsic direction, having a 'plus' and a 'minus' end. Some molecular motors such as cytoplasmic dynein walk to the minus end, while others such as conventional kinesin walk to the plus end. Cells typically have an isopolar microtubule network. This is most pronounced in neuronal axons or …
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Structural studies of dynein’s interaction with microtubules and herpesvirus
The dynein family is a group of minus-end directed microtubule motor proteins vital for long- range cytoplasmic cargo transport and ciliary beating. The dynein heavy chain consists of an N- terminal tail domain responsible for cargo binding and dimerization, and a C-terminal motor domain. In order …
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Effects of Cysteine Modification on Microtubule-Motor Protein Function and Tubulin Assembly
… which NEM affects motor function and inhibits MT minus end assembly. Recombinant motor domains of Drosophila kinesin (DK350 and DK375), Ncd (MC1), and squid kinesin (p181) were modified by NEM. NEM treatment was shown to affect the binding of MC1, but not recombinant kinesin proteins to MTs in the …
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Relationship between Hepatitis B Virus Core Particles and Intracellular Motility
… of mitochondria in 30 min, suggesting that the minus end-directed motor protein, dynein, might be actively involved. ER and peroxisome are aggregated to perinuclear region like mitochondria in HBV replicating stable cells. Also, HBV core particles and MTs were colocalized in HBV replicating …
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Breaking Down Microtubule Formation: Characterizing the Biochemical and Cellular Functions of the γ-Tubulin Ring Complex
… of new microtubules, modulating microtubule minus-end dynamics by acting as a minus-end cap, and anchoring microtubules to specify their cellular localization. These three major activities of the γ-TuRC, nucleation, capping, and anchoring, contribute to the dynamic nature of individual …
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Force studies of multiple kinesin-1 and EG5 molecular motors
… structures through a balance between plus-end directed and minus-end directed forces along the filaments. Therefore, it is impmiant to understand how multiple kinesin-1 and Eg5 motors withstand competing forces, their structural rigidity under such loads, and how quickly they are able to …
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Role of Microtubule Motor Proteins in Adenoviral Infections
… into clathrin-coated pits. Conditions inside the endosome trigger escape of the virions from the organelle through membrane disruption 5-15 min post-infection and most capsids gain access to the nucleus about 30-45 min thereafter. Interestingly, adenovirus relies on the microtubule (MT) network, …
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Role of Molecular Motors and Maps in Spindle Dynamics and Chromosome Segregation in the Fission Yeast Schizosaccharomyces Pombe
… localize to the spindle poles and focus the MT minus ends. Their absence leads to pole and MT defocusing, resulting in protrusion of MT minus ends due to cut7p-dependent pushing forces at the spindle midzone. Infrequent long MT minus end protrusions can push the already separated chromosome mass …
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Microtubule Motors Drive Nuclear Dynamics and Positioning in Developing Skeletal Muscle Cells
… and cytoplasmic dynein. Depletion of the plus-end directed motor kinesin-1 abolishes nuclear rotation and significantly inhibits nuclear translocation, resulting in the abnormal aggregation of nuclei near the midline of the myotube. Loss of the minus-end directed dynein motor also inhibits …
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Regulatory mechanisms of MYO6 motor domain activity and tail interactions
… their unique reversed directionality towards to minus-end on actin filaments compared to other classes. It can function as a -retrograde- transporter or tether in a wide range of biological processes. Dysfunction of myosin VI (MYO6) is linked to multiple pathologies, such as hearing loss, …
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NF1 AS A REGULATOR OF CYTOSKELETON DYNAMICS AND BIOMARKER FOR DECISION-MAKING IN HER2-POSITIVE BREAST CANCER
… but sparse evidence suggests additional Ras-independent roles. The interaction of its product neurofibromin with both microtubule (MT) and actin cytoskeleton remains poorly characterised to date but may be of particular therapeutic interest as NF1 is somatically mutated across multiple tumour …
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An Examination Of The Cytoplasmic Dynein Stepping Mechanism At The Single Molecule Level
… dynein walks along microtubules toward the minus end and is responsible for a wide range of cellular functions including cargo transport and chromosome alignment during cell division. This work employs a position and rotational tracking method, polarized total internal reflection …
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Mechanisms of dynein-based mRNA transport processes in human cells
… This thesis investigates how the major minus-end-directed microtubule motor, dynein, traffics mRNAs in human cells. A co-immunoprecipitation-based proteomics approach was first employed to identify candidate adaptors between dynein and mRNA in HeLa cells. Analysis of the interactomes of …
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Chemical Biology of Dynein
… cellular cargoes towards the microtubule minus-end. Despite its essential role in intracellular transport, dynein remains the least understood cytoskeletal motor. With speeds >25 μm/min in cells, dynein's cellular functions are challenging to study using genetic approaches, such as CRISPR …
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Distinct Roles for Dynein Regulatory Proteins NudE and NudEL in Brain Development
… important, since it is the major microtubule minus-end directed motor in cells. Dynein is a large, complex structure comprising several subunits and binding partners. Its function is critical for multiple stages of brain development. The dynein regulatory proteins NudE and NudEL have been …