Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"MinION"”.
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Approaches to mitochondrial genome sequencing using the oxford nanopore minion device
… sequencing sans PCR using the Oxford Nanopore MinION. Our hypothesis was that results generated from native DNA sequencing would be concordant to sequencing results generated from PCR-enriched libraries. Error-rates of 1.47% (enriched) and 1.26% (native) were observed when sequencing the …
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Dynamic Task Allocation in Partially Defined Environments Using A* with Bounded Costs
… allocation problem as a graph search problem, Minion Task, is not only capable of finding and executing tasks, but is also capable of optimizing costs across a range of parameters and of considering constraints on the order that tasks may be completed in. Minion task consists of four key phases …
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Equine Herpesvirus Type 1: Filling Gaps Toward Improved Outbreak Management
… has been developed in a handheld device, called MinION, that provides thorough output in a timely manner. When combined with LAMP, it has been referred to as LAMPore. The first objective of our work was to estimate the prevalence of latent EHV-1 and compare the frequency of each variant in the …
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Understanding how microbial community contributes to wastewater treatment performance in constructed wetlands within Scotch whisky distilleries
… and evaluating taxonomic results using ONT MinION and Illumina MiSeq. The research also examines spent lees treatment in mesocosms with various substrates and biochar, analysing biofilm diversity, microbial dynamics, and biochar's impact. Additionally, it assesses seasonal treatment …
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Detection of Nucleic Acid Disease Biomarkers Using Solid-State Nanopores
… With the release of Oxford Nanopore Technology’s Minion sensor in 2014, that goal has been achieved. While this device utilizes biological nanopores, Solid-State Nanopore sensors were also initially investigated to develop sequencing devices. Significant efforts are being made to this day towards …
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A Study in Object Detection and Classification Performance by Sensing Modality for Autonomous Surface Vessels
… Embry-Riddle Aeronautical University’s (ERAU) Minion platform and 2024 Maritime RobotX Challenge data, this study evaluates the detection of six maritime object categories using two representative models. YOLOv8 provides a neural network vision-based method, and GB-CACHE provides a …
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Blood, Tears, and Sweat: An Intersectional Excavation of the Literary Vampire in Neoliberal Discourse
… Jewelle Gomez's The Gilda Stories, L.A. Banks's Minion, and Charlaine Harris's Dead Until Dark. As the ideology of neoliberal individualism has shaped the dominant discourse of the United States, neoliberal individualism has also remade the discourse of monstrosity with regard to vampires. The …
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Mixed Strain Identification of Porcine Reproductive and Respiratory Syndrome Virus in Multiplexed Samples using Nanopore Sequencing
… capacity of Oxford Nanopore Technology's MinION next-generation sequencer. We have developed a novel primer set designed to span Open Reading Frames 3 through 7 of the PRRSV genome, which has allowed for multiplexing of samples, thus reducing individual cost of testing, while yielding …
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Legume-rhizobia interactions in a complex microbiome
… levels. The use of long reads with the MinION DNA sequencer also allowed the potential of identification of horizontal gene transfer events. None were detected in an initial screen, but a framework for further inspection of this dataset for such events is described.
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Legume-rhizobia interactions in a complex microbiome
… levels. The use of long reads with the MinION DNA sequencer also allowed the potential of identification of horizontal gene transfer events. None were detected in an initial screen, but a framework for further inspection of this dataset for such events is described.
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Marine snow tracking stereo imaging system
… data in preparation for eventual deployment the Minion platform, a Lagrangian float designed to quantitatively explore the Biological Carbon Pump. An evaluation of the system's efficacy in estimating particle concentration and sinking rate, and ultimately estimate the particle flux, indicates a …
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Characterizing the Microbiome of Scottish Freshwater Pearl Mussels (Margaritifera margaritifera): A Metabarcoding Analysis using Next-Generation Sequencing
… Two sequencing platforms were utilized: the MinION (Oxford Nanopore Technology) for full-length amplicons and MiSeq (Illumina) for short amplicons, enabling a comparative analysis of platform performance at the species level. Results revealed that the mussel microbiome was distinct from that …
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High Throughput Sequencing of SARS-CoV-2
… coverage. By comparing two sequencing platforms, MinION and Illumina, we determined that the Illumina platform was the preferred method based on error rates, sequencing read depth, and multiplexing compatibility. Using our pipeline, we calculated the mutation rate of SARS-CoV-2 in twelve …
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Integrating bioinformatic approaches to promote crop resilience
… sequencing from the Oxford Nanopore Technologies MinION, and short-read data, to rapidly assembly a cost-effective, contiguous and relatively complete genome. Here we uncovered hidden variation in a well-known plant pathogen finding that the genome was 92% bigger than previous estimates with more …
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Managerial autonomy vs. public accountability in public enterprises
Thesis: M.S., Massachusetts Institute of Technology, School of Industrial Management, 1964
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The broken genome: Merging cutting edge technologies for a molecular understanding of the Flock House virus defective interfering particle.
… resolution) and Oxford Nanopore Technologies’ MinION (to characterize full-length and defective genomes) I have characterized the step wise progression of DI-RNA formation and the species distribution of these genomes. I observed a rapid accumulation of mature DI-RNAs suggesting that …
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The Convoluted Origin and Diversification of Strangler Figs in the Neotropics
… sequencing platforms (PacBio Sequel II, Nanopore MinIon, and Illumina Miseq). With this data I was able to assemble 92% of the F. velutina genome. 56.4% of the genome was annotated with either gene predictions or repetitive elements. I also explore the applicability of this genome as a reference …
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Computational Tools for Improved Detection, Identification, and Classification of Plant Pathogens Using Genomics and Metagenomics
… using the Oxford Nanopore Technologies (ONT) MinION for fungal and bacterial plant pathogen detection and precise identification are demonstrated. To improve the classification of the strains belonging to the Ralstonia solanacearum species complex (RSSC), we performed a meta-analysis using a …
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