Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Migration Assay"”.
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Modulation of Brain Inflammation Induced by Interaction between Astrocytes, Neutrophils and Monocytes
… media 13 6. ELISA (Enzyme-linked immunosorbent assay) 13 7. Migration assay 14 8. Statistical analysis 14 III. RESULTS 16 A. Interaction between astrocytes and neutrophils 16 1. Effect of neutrophils on morphology of astrocytes 16 2. Effect of neutrophils on inflammatory responses of astrocytes …
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The effect of low level light therapy on wound healing: in vitro study with normal and keloid fibroblast
… and keloid fibroblasts using EZCyTox viability assay after 24 hours from irradiation with 3, 10, and 30 J/cm2 of each wavelength. Proliferation rate of both fibroblasts relative to nonirradiated controls were measured using trypan blue exclusion assay after 24 hours from irradiation with 3, 10, …
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Role Of Gravin In Endothelial Wound Healing
… regulators is an important factor in cell migration. SSeCKS, the rodent orthologue of gravin is reported to be involved in cell migration and regulation of actin cytoskeleton in cancer cell lines. Further, Western blot analysis, in the current study reveals that gravin upregulation in EC is …
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Characteristic analysis of mobility inducers for neural stem cells
… activation of signal pathway for mobility. In migration assay using functional blocking antibody, we identified that integrin αvβ5 interacted with CM-1 and induced functional cell migration in human glioblastoma primary cultured cells and rat glioma cells as well as human NSCs. In the presence …
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B-RafV600E에 의한 갑상샘 발암과정에서 Thyroid stimulating hormone (TSH) 의 역할연구
… 6 7. Immunoprecipitation-Phosphatase Activity Assay 6 8. Measurement of Ras Activity 7 9. Real-Time PCR Analysis 7 10. Measurement of Reactive Oxygen Species 8 11. RNA Interference 8 12. Senescence-Associated β-Galactosidase Staining 9 13. Analysis of DUSP6 Protein Stability 9 14. …
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TRANSFORMATION OF PRIMARY HUMAN HEPATOCYTES IN HEPATOCELLULAR CARCINOMA
… characteristics was studied with a trans-well migration assay. We observed that HCC cells maintained their morphology and unmodified neoplastic characteristics when cultured. Cells isolated from CP, showed a progressive morphologic transformation in HCC-like cells accompanied by modification of …
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Investigating Mechanical Strain-Induced Phenotypic Changes on Prostate Cancer Cell Toward Metastasis Using a Three-Dimensional <i>In-Vitro</i> Model
… potential was investigated using a custom cell migration assay. Results showed static loading significantly increases cell elongation, as compared to control and cyclic loading conditions (p<0.001). Increased cell elongation is indicative of the morphological changes associated with …
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Yap and Taz Are Required For Neural Crest-Derived Cardiovascular Development
… RNA-sequencing data together with transwell migration assay, scratch assay, and <em>ex vivo </em>culture, indicate a potential role for <em>Yap/Taz</em> in regulating NC migration. Together, our data indicate that <em>Yap/Taz</em> play a critical role in proper cardiac NC-derived cardiac …
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The Role of Post-Translation Regulation of Twist Expression In The Tumor Progression of Squamous Cell Carcinoma of Head and Neck
… by IL-6 which we have found stimulates cell migration and soft agar growth in SCCHN. Our data show that IL-6 increases TWIST expression in a transcription-independent mechanism in many SCCHN cell lines. Further investigation reveals TWIST can be phosphorylated upon IL-6 treatment. By …
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Haematopoietic progenitor cells in carotid disease
… endarterectomy (CEA). Methods Initially the assay conditions to measure HPC number and function were optimized. HPC numbers were measured by flow cytometry (CD133+ve/CD34+ve) and early colony forming unit assay (eCFU). HPC function was measured by migration assay and by staining for …
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The Brain Invaders: Widespread Neuronal Dispersion in Developing and Adult Mammalian Brain
<p>Cell migration is the principal determinant of the final brain cytoarchitecture. However, we have only limited knowledge about the migratory pathways and mechanisms utilized during brain development. In my thesis I present new and unexpected findings about neuronal migration in the developing …
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The effects of mechanical forces on lymphocyte adhesion and migration
… of mechanical forces on cellular function. To assay lymphocyte function, adhesion and migration experiments were preformed on extracellular matrix-coated surfaces. A cell migration assay was developed where cells were tracked on a two-dimensional surface. Cell trajectories were reconstructed …
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Investigating the movement of intestinal intraepithelial lymphocytes using an organoid co-culture system
… regulated by IL-15 that could be involved in IEL migration. The expression of several adhesion molecules and chemokine receptors was either upregulated or downregulated which showed a potential involvement in IEL movement and retention within the epithelium. I showed that one way in which IL-15 …
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Characterization of human foreskin Langerhans cells
… FS cells were extracted using crawl (migratory) assay and liberase enzyme digestion. Langerhans cells were isolated by density gradient centrifugation, sorted, quantified and immune-profiled by flow cytometry. CD1a and CD207 were used to identify Langerhans cells while HLA-DR, CD80, CD86 and CD40 …
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The role of staphylococcal superantigen-like protein 7 (SSL7) in immune evasion
… of the bacteria in a human whole blood killing assay. Moreover, in an in vitro migration assay and in vivo murine model of peritoneal inflammation, SSL7 inhibited the chemotaxis of inflammatory cells in response to a pathogenic stimulus. Protection against blood killing and reduction of …
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Bioastronautics: Biological Modeling of the Neural Response to Space Radiation and its interaction with Alzheimer's Disease Risk Genes
… of in vitro systems to incorporate a microglial migration assay and to better model the low fluence nature of the space radiation environment while still retaining compatibility with the NSRL accelerator. We then show proof-of-concept for an accelerator-compatible, rapid differentiation neuron …
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Anthelmintic Screening, Phytochemical Analysis and Cytotoxicity Determination of the Indigenous Plants of the U.S.A. Northern Great Plains
… plant extracts were screened with an egg hatch assay, and the 8 plants with the highest activity in this assay were further screened with a larval migration assay.<em> Ericameria nauseosa and Rhus aromatica</em> exhibited the highest activity in both assays, and were, therefore, further …
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A Systems-Based Approach to Examine the Molecular Basis of Interactions between Human Monocytes and Human Micro-Vascular Endothelium
… In order to facilitate this, a novel in vitro migration assay was developed to enable simultaneous monitoring of the locomotory behaviour of all three monocyte types over endothelial monolayers by live-cell, fluorescent time-lapse imaging, with the whole system subjected to physiologically …
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Investigating the role of primary cilia loss in intrahepatic cholangiocarcinoma
… organoid conditioned media (CM). In a Transwell migration assay, I showed that neutrophils exposed to non-ciliated CM migrated significantly less than when cultured in the presence of ciliated CM, suggesting that PC-loss on cancer cells directly impacts neutrophil migration. The work presented …
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Injectable chemokine-releasing gelatin matrices for enhancing endogenous regenerative responses in the injured rat brain
… material that was permissive for proliferation, migration and differentiation of adult neural progenitor cells (aNPCs). We also synthesized dextran sulfate/chitosan polyelectrolyte complex nanoparticles (PCN), which could encapsulate SDF-1[alpha] efficiently and sustain its release for 4 weeks. …
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