Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Migrating cells"”.
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The Function of CARMIL1 in Migrating Cells
… of the Capping Protein- CARMIL interaction in migrating cells. I establish the CARMIL-CP complex as a key regulator of lamellipodial actin assembly and of lamellipodial dynamics. Membrane ruffling at the leading edge of motile cells and macropinocytosis were also found to be dependent on the …
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HDAC6 activity is required for efficient polarization and intracellular transport of organelles in directionally migrating cells
… important role in polarization of directionally migrating cells, we hypothesized that HDAC6 activity regulates important MT dependent aspects of directional cell motility. We were able to test this hypothesis by observing cellular polarization and organelle and vesicle transport in the presence …
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Radial Glia and the Migration of Young Neurons in the Adult Avian Brain
… integrate them into functional circuits. These cells are born in the ventricular zone (VZ) of the lateral ventricle, sometimes up to 5-6 mm away from the location were some of these neurons finally mature (Nottebohm, 1985). How do new neurons find their way through the adult parenchyma away from …
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Upstream Migration Under Shear Flow: Force Generation and Molecular Regulation
… force to this process, the forces upstream-migrating cells generate at the cell-substrate interface have not been directly measured during sustained upstream migration, and the molecular machinery responsible for producing them remains uncharacterized. This dissertation addresses both gaps …
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Detailed analysis of Lmx1b expression in multiple organs and its role in dorso-ventral patterning with emphasis on myogenic precursor cells
… development and restricted to luminal epithelial cells. Mice targeted for Lmx1b lack mammary glands. These findings indicate that Lmx1b plays a pivotal role in mammary gland development. Indeed, female patients with NPS display poor breast development (Sweeney et al. 2003). It is shown that Lmx1b …
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Die Verteilung des cellular retinoic acid binding protein-I (CRABP-I) und der Retinoidrezeptoren im optischen Tektum während der Entwicklung des Haushuhns Gallus gallus
… protein for RA, which tends to be expressed in cells that are responsive to RA. My data show immunoreactivity of CRABP-I in the tectal anlage at E 2.5 and during the entire period of embryonic development. It was found in differentiating neurons of the generative zone, in migrating cells of the …
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An Optical Flow Based Methodology for Visualizing Dynamic Sucellular [sic] Organiztion [sic] Demonstrated Through Profilin and Rho GTPase Microdomains
… was previously achieved for the leading edge of migrating cells in select cases. Importantly no strategy existed to simultaneously analyze every subcellular location. Building on existing optical flow based non-linear image registration we developed an approach to remap a migrating cell to a …
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Mechanisms of Eph/ephrin mediated cell-cell communication
… ligands mediate cell-cell repulsion to guide migrating cells and axons. A peculiarity of this signaling system is that both receptors and ligands can transduce signals into the cell resulting in a bidirectional signaling mode. An important step of ephrinB ligand ‘reverse signaling’ is the …
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Tracking Nanoparticles going into the nucleus of the cell
… of nanoparticles into the nucleus of migrating versus no-migrating MCF-7 human breast cancer cell line. Initially, by using the 18 nm Cy5 labelled fluorescent silica nanoparticles, it was found that behaviour of nanoparticles internalization into the nucleus of MCF-7 breast cancer cell …
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Automated Identification and Tracking of Motile Oligodendrocyte Precursor Cells (OPCs) from Time-lapse 3D Microscopic Imaging Data of Cell Clusters in vivo
… into the migration of Oligodendrocyte precursor cells (OPCs) and its role in the central nervous system. However, current practice of image-based OPC motility analysis heavily relies on manual labeling and tracking on 2D max projection of the 3D data, which suffers from massive human labor, …
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The Effects of Acetylenic Tricyclic Bis-(Cyano Enone) on Cell Migration
… to form actin stress fibers, which prime tumour cells for migration. In addition to the actin cytoskeleton, I demonstrated that TBE-31 alters microtubule dynamics and organization. Microtubule-dependent trafficking was also shown to be disrupted by TBE-31, and the localization of the polarity …
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An in vitro cell lineage model to identify alterations in the differentiation program of neuroblastoma
… solid tumor that arises from trunk neural crest cells (tNCCs), a transient cell population involved in the development of the sympathetic nervous system. The tNCCs are migrating cells that differentiate into sympathoadrenal precursors (SAPs) and Schwann cell precursors (SCPs). The SAPs are the …
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Detection and tracking of cells and focal adhesions in microscopy images
… perform detection, segmentation, and tracking of cells or subcellular structures in time-lapse microscopy image sequences to facilitate efficient analysis of these datasets. Furthermore, a secondary purpose of the thesis deals with the lack of annotated data in the biomedical field. <br/><br/>In …
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Development and application of correlative STED and AFM to investigate neuronal cells
… was combined with a commercial AFM to study live cells. Using the recently developed SiR-actin and SiR-tubulin dyes and AFM probes optimised for live cell mechanical property studies, images of the actin and tubulin cytoskeleton were correlated with AFM topography and mechanical stiffness …
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Analysis of the role of Drosophila IMP in actin mRNA localization and cell migration
… it localizes actin mRNA to the leading edge of migrating cells. mRNA in situ for the two cytoplasmic actins in Drosophila (actin42A and actin5C) shows that both are localized to the front of the migrating border cell cluster as is IMP protein. Border cells mutant for either actin42A or actin5C …
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Bleb-driven chemotaxis in Dictyostelium discoideum
Migrating cells have two basic ways of extending their leading edge: by dendritic actin polymerization beneath the membrane, or by fluid pressure, which produces blebs. Most cells are believed to move using actin-driven projections, but in more physiological conditions, blebbing motility is also …
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Antibody technology identifies distinct mechanisms controlling the lengths of filopodia-like structures
… actin and extend beyond the leading edge of migrating cells. Filopodia are thought to facilitate both sensing of signaling molecules and the movement of cells towards them. Profuse filopodia formation is seen in metastatic cancer cells and in diseases causing intellectual disability. …
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Structural Mechanisms of F-actin Mechanochemical Regulation and Flexible Network Assembly
… protrusions localized at the leading edge of migrating cells, which play a prominent role in the dissemination of metastatic cancer cells. I first focused on T-plastin, an ABP that loosely bundles F-actin at the roots of filipodia. By adapting my neural-network–based picking scheme to identify …
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Mesenchymal stromal cells in malignant glioma - Functions and therapeutic potential
… GBM is its infiltrative growth. As the tumor cells leave the tumor bulk and migrate into the normal brain parenchyma, it is impossible to reach them with the current standard treatments. Hence, even after treatment, some tumor cells will remain in the brain and eventually give rise to a new …
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